Antifungal activity of chitosan oligomer prepared using chitosanase of Aeromonas media KLU 11.16

Abstract

Chitosan oligomer is a hydrolysis product of chitosan which can be prepared by enzymatic method, including chitosanase that may be produced by bacteria. Previously, chitosanase from Aeromonas media KLU 11.16 has been purified and characterized. The aim of this study was to analyze the bioactivity of chitosan oligomer prepared by using Aeromonas media KLU 11.16 chitosanase, as antifungal. Chitosan oligomer was prepared from shrimp waste chitosan, and hydrolyzed by 8U chitosanase per gram chitosan. The reaction time was for 2, 3 and 4 hours at 30°C. The oligomer produced then determined their yields and viscosities; and was identified by using a thin layer chromatography (TLC). Antifungal activity test against Aspergilus flavus, A. niger, Eurotium amstelodami and Emericella nidulans was performed on selected oligomers based on consideration of yield and TLC result, with oligomer concentrations of 0; 50; 100 and 200 ppm. The result showed that hydrolysis chitosan by Aeromonas media KLU 11.16 chitosanase for 4 hours reduced the viscosity from 187.5 to 13 cPs, and produced oligomers 96.14% of yield. The oligomers identified by TLC were monomer (1 unit) to hexamer (6 unit), and showed their antifungal activity against the four fungi tested. The best inhibition was showed by 200 ppm of oligomer against A. flavus.

Characteristics of Chitooligosaccharides Hydrolysate from Crab Shell (Portunus Pelagicus) Waste by Chitosanase Hydrolysis

“…The higher decrease in chitosan viscosity (up to 99.5%) at 16 hours of hydrolysis use a similar enzyme (activity 36.212 U/g) on a larger scale (10-15 times capacity) (Alain Gohi et al, 2016). Hydrolysis of chitosan (DD 74.6%) using pepsin reduced the viscosity by 86% after 1 hours (Yusro Nuri Fawzya et al, 2019). Based on Table 2, the yield of chitosan obtained in this study was 5.17%.…”

Bioprocessing of Squid Pens Waste into Chitosanase by Paenibacillus sp. TKU047 and Its Application in Low-Molecular Weight Chitosan Oligosaccharides Production

“…The obtained COS appeared as a yellowish powder with a yield of 68.44%. In previous studies, the COS production yield by the enzymatic method varied-for example, Streptomyces griceus chitosanase was 46.3%; lipase A from Aspegillus niger was 42.5%; Trichoderma viride cellulase was 46.1%; pork pepsin was 52.2% [69]; B. cereus S1 chitosanase was 100% [70]; a combination of chitinase and snailase was 24% [71]; Aeromonas media KLU 11.16 chitosanase was 96.14% [72]; and Bacillus pumilus BN-262 chitosanase was 40% [73]. This indicated that the COS production yield of Paenibacillus sp.…”

Selection of stain fungi on rubberwood (Hevea brasiliensis) and its growth response against chitosan

“…The presence of chitin plays a role in helping maintain cell walls, whereas glucans regulate the flexibility and consistency of cell walls (Aranda-Martinez et al 2016). The inhibition growth was suspected by the interaction between chitosan and the plasma membrane which increased the oxidative stress (Fawzya et al 2019). Another research informed that chitosan damage membranes and penetrates into fungal cells, thereby damaging intracellular composition, for instance, inhibit the absorption of nutrients, interfere with mRNA and protein synthesis (El-Guilli et al 2015).…”