Antifungal activity of Brazilian medicinal plants involved in popular treatment of mycoses

Cruz, Miranda; Santos, Patrícia; Barbosa, Andriele Mendonça; Melo, Daniela Oliveira de; Alviano, Celuta Sales; Antoniolli, Ângelo Roberto; Alviano, Daniela Sales; Trindade, Rita C.

doi:10.1016/j.jep.2006.12.005

Cited by 92 publications

(85 citation statements)

References 17 publications

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“…However, the oil of the same plant evaluated with the poisoned food technique completely inhibited mycelial growth of this filamentous fungi at 100 µg/mL (Kumar et al, 2007). This difference in antifungal activity between essential oils from plants of the same species can be explained by the chemotype (chemical composition), geographical region of plant collection, age of plant, extraction method of oils, method used to assess the antifungal activity or by the intra or inter species differences (Samaranayake et al, 1995;Cruz et al, 2007;Maksimovic et al, 2008). Sharma & Tripathi (2008) have shown that the antifungal activity of essential oils is better evaluated in liquid than in solid media, since in the latter, the hydrophobic nature of the majority of essential oil components hamper uniform diffusion of these substances through the agar medium (de Cerqueira et al, 2007).…”

Section: Resultsmentioning

confidence: 98%

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

Correa-Royero¹,

Tangarife²,

Durán³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Atividade antifúngica in vitro e os efeitos citotóxicos de óleos essenciais e extratos de plantas medicinais e aromáticas contra Candida krusei e Aspergillus fumigatus" As plantas são geralmente utilizadas na medicina tradicional como agentes antimicrobianos e seus óleos essenciais e extratos foram conhecidos por possuir atividade antifúngica. O objetivo deste estudo foi avaliar in vitro a atividade de 32 óleos essenciais e 29 extratos contra Candida krusei e Aspergillus fumigatus, bem como o efeito citotóxico em células Vero. A curva do tempo-morte e a interação entre antifúngicos e Chenopodium ambrosioidese do extrato de Myrcia cucullata mostraram atividade antifúngica contra C. krusei (geometric means of the minimal inhibitory concentration [GM-MIC] 7,82 e 31,25 µg/ mL, respectivamente). Lippia citriodora foi ativa contra C. krusei e A. fumigatus (GM-CIM = 99,21 µg/mL e 62,5 µg/mL, respectivamente). Os testes de tempo-morte feitos com óleo de C. ambrosioides mostraram atividade fungicida em 4x MIC. A interação do óleo C. ambrosioides com itraconazol e anfotericina B foi testada pela técnica de xadrez. Nenhuma interação foi detectada pela combinação do óleo C. ambrosioides com anfotericina B e itraconazol (intervalo fractional inhibitory index [FICI] = 1,03-1,06 e 1,03-1,00, respectivamente). Os ensaios de citotoxicidade para todas as amostras foram realizadas com MTT. Apenas os óleos Hedyosmun sp. e L. dulcis foram citotóxicos.Unitermos: Candida krusei, Aspergillus fumigatus, óleos essenciais, extratos, curva do tempomorte, técnica de xadrez. ABSTRACT:The plants are usually used in traditional medicine as antimicrobial agents and their essential oils and extracts have been known to possess antifungal activity. The aim of this study was to evaluate the activity of 32 essential oils and 29 extracts in vitro against C. krusei and A. fumigatus as well as the cytotoxic effect on Vero cells. Time-kill curve and interaction between antifungal and the most active sample against C. krusei, was also evaluated. The oils from C. ambrosioides and the extract of M. cucullata showed antifungal activity against C. krusei (GM-MIC 7.82 and 31.25 µg/mL, respectively). L. citriodora was active against C. krusei and A. fumigates (GM-MIC = 99.21 µg/mL and 62.5 µg/mL respectively). Time-kill assays done with C. ambrosioides oil showed fungicidal activity at 4x MIC. The interaction of C. ambrosioides oil with itraconazole and amphotericin B was tested following the chequerboard technique. No interaction was detected for the combination of C. ambrosioides oil with amphotericin B and itraconazole (FICI range = 1.03-1.06 and 1.03-1.00, respectively). Cytotoxicity assays for all samples were carried out with MTT. Only the oil from Hedyosmun sp. and L. dulcis were cytotoxic.

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Section: Resultsmentioning

confidence: 98%

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

Correa-Royero¹,

Tangarife²,

Durán³

et al. 2010

Rev. bras. farmacogn.

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“…Há uma disposição mundial quanto ao uso de antimicrobianos naturais no combate a microrganismos causadores de doenças [39,40]. Neste sentindo, tanto S. aureus que pode provocar doenças, que vão desde uma simples infecção (espinhas, furúnculos e celulites) até infecções graves (pneumonia, meningite, endocardite, síndrome do choque tóxico, septicemia e outras) [41], E.coli que pode ocasionar doenças em superfícies mucosas ou ainda se disseminar através do organismo, implicando em processos de infecções gastrointestinais, urinárias e meningite [42,43], quanto outras bactérias patológicas avaliadas neste estudo, podem ser tratadas utilizando chás medicinais de S. purpurea ou associadas com antibióticos durante o tratamento, visto ser um hábito comum de muitas pessoas atualmente.…”

Section: Resultsunclassified

Avaliação da atividade genotóxica, citotóxica e antimicrobiana da infusão das folhas de Spondias purpurea L.

Santos

Marisco

2017

Sci. Plena

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_____________________________________________________________________________O uso de plantas medicinais cada vez mais é mostrado como uma fonte alternativa para tratamento de doenças. Este trabalho visou determinar o nível genotóxico e citotóxico da infusão das folhas de Spondias purpurea L., investigar o potencial antimicrobiano e conhecer o perfil cromatográfico de compostos presentes na infusão. Foram realizados os testes Allium cepa e Artemia salina para avaliar a genotoxicidade e citotoxicidade, respectivamente. Os métodos de difusão em disco e concentração inibitória mínima (CIM) foram usados para determinar a atividade antibacteriana nas bactérias Staphylococcus epidermidis, S. aureus, Escherichia coli, Salmonella enterica sorotipo Choleraesuis, Pseudomonas aeruginosa e Klebsiella pneumoniae. No teste de A. cepa, a análise estatística não indicou diferenças significativas do índice mitótico entre os tratamentos e o controle. O teste de A. salina mostrou que a infusão apresentou toxicidade frente aos náuplios a partir da dose 10µg/mL. Em relação à atividade antibacteriana, houve presença de halos de inibição contra as bactérias S. aureus e E. coli, e quando submetidos ao CIM, a infusão apresentou atividade antibacteriana contra todas as bactérias testadas. Sendo assim, a partir desse estudo pode-se inferir que a infusão (chá) das folhas da planta S. purpurea se mostrou como uma fonte medicinal não genotóxica para o teste A. cepa, bem como uma possível fonte antitumoral indicada pelo teste A. salina, além de uma promissora fonte antibacteriana.Palavras-chaves: Genotoxicidade, Antimicrobiana, Seriguela.The use of medicinal plants is increasingly shown as an alternative source for the treatment of diseases. This study aimed to determine the level genotoxic and cytotoxic infusion of the leaves of Spondias purpurea L., study the antimicrobial potential and know the chromatographic profile of the compounds present in the infusion. Tests were performed Allium cepa and Artemia salina to assess the genotoxicity and cytotoxicity, respectively. The disk diffusion method and the minimum inhibitory concentration (MIC) they were used to determine the antibacterial activity in bacteria Staphylococcus epidermidis, S. aureus, Escherichia coli, Salmonella enterica serotype Choleraesuis, Pseudomonas aeruginosa e Klebsiella pneumoniae. On test A. cepa, statistical analysis indicated no significant differences in mitotic index between treatment and control. The test of A. salina showed that infusion toxicity has opposite from the nauplii dose 10mg / ml. With regard to antibacterial activity, there was the presence of halos of inhibition against the bacteria S. aureus e E. coli, and when subjected to MIC infusion showed antibacterial activity against all tested bacteria. Thus, from this study can be inferred that the infusions (tea) from the plant leaves S. purpurea showed as a non-genotoxic medicinal source for testing A. cepa, as well as a possible antitumor source indicated by the test A. salina, as well as a promising anti...

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“…The discovery of new antifungal agents remains an important challenge for the scientific community and traditional medicinal plants may furnish promising *Corresponding author E-mail: phtech@uol.com.br. Tel: +55 81 2126-8511; Fax: +55 812126-8510 material for the development of antifungal drugs (Cruz et al, 2007;Souza et al, 2010). Although the number of reports on species distributed by regions or biomes remains relatively small, interest in medicinal plants from northeastern Brazil has been increasing, especially in plants from the semi-arid Caatinga biome (Albuquerque et al, 2007a).…”

Section: Introductionmentioning

confidence: 99%

“…Although the number of reports on species distributed by regions or biomes remains relatively small, interest in medicinal plants from northeastern Brazil has been increasing, especially in plants from the semi-arid Caatinga biome (Albuquerque et al, 2007a). Several studies have described this region's rich diversity of plants used for medicinal purposes, widely employed by the traditional communities until the present day (Almeida et al, 2005;Albuquerque et al, 2007a,b;Agra et al, 2007Agra et al, , 2008Cruz et al, 2007;Silva et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity of medicinal plants from Northeastern Brazil

Ferreira¹,

Santiago²,

Langassner³

et al. 2013

J. Med. Plants Res.

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The aim of this work was to find how medicinal plants play an important role as source of new bioactive molecules. To evaluate the antifungal activity of 10 medicinal plants from northeastern Brazil, traditionally used as anti-infective agents. The activity of 30 crude extracts (water; ethanol:water, 1:1; acetone:water, 1:1) against four standard species of Candida yeasts (Candida albicans ATCC 90028, Candida dubliniensis ATCC 7289, Candida glabrata ATCC 2001 and Candida krusei ATCC 6258) was investigated by the Minimal Inhibitory Concentration (MIC), using the microdilution method and the working range used was from 1.95 to 1000 μg/mL. Extracts from leaves of Eugenia uniflora (Myrtaceae), stem bark of Caesalpinia ferrea (Caesalpinaceae) and leaves of Psidium guajava (Myrtaceae) showed significant activity against all yeasts evaluated. The best antifungal activities were achieved against C. glabrata and C. krusei by E. uniflora extract (MIC = 15.62) and followed by extracts from C. ferrea and P. guajava (MIC ranged from 15.62 to 250 µg/mL). E. uniflora also showed fungicidal properties against all yeasts, especially against Candida dubliniensis. This study identified plant species that may be candidates for the development of alternative treatments for candidiasis.

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Antifungal activity of Brazilian medicinal plants involved in popular treatment of mycoses

Cited by 92 publications

References 17 publications

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

In vitro antifungal activity and cytotoxic effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus

Avaliação da atividade genotóxica, citotóxica e antimicrobiana da infusão das folhas de Spondias purpurea L.

Antifungal activity of medicinal plants from Northeastern Brazil

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