Antifreeze protein detection using Rhodamine B as photoluminescence label in porous silicon

Zhang, Hong Yan; Jia, Zhenhong; Lv, Xiaoyi; Hou, Junwei; Liu, Xiaojing; Ma, Junsheng; Zhou, Jun

doi:10.1016/j.cap.2012.11.014

Cited by 15 publications

(9 citation statements)

References 31 publications

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“…In addition, the optical properties of PSi can be easily tuned depending on the type of the structure produced by electrochemical etching, such as monolayer, bilayer, Bragg reflector and microcavity [7]. For this reason, numerous authors have implemented this material for biosensing and sensor applications, including the detection of metal ions [8], phenol [9], complementary DNA [10], gas molecules such as NO2 [11], proteins [12], and bacteria [13].…”

Section: Introductionmentioning

confidence: 98%

Porous Silicon Nanostructured Materials for Sensing Applications: Molecular Assembling and Electrochemical or Optical Evaluation

et al. 2016

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Porous silicon (PSi) combines the potential of miniaturization with a very large surface area. The PSi surface can be chemically modified resulting in a high sensitivity (low detection threshold) device for chemical and biomolecular sensing. In previous work, we have shown that redox proteins and fluorescent ligands can be infiltrated into PSi (PSiMc) structures. The hybrid devices have shown interesting new properties produced by the coupling of the individual properties of PSi nanostructures and the modifiers. In this work, we have obtained a PSiMc/redox protein bioelectrode, which presents a quasi-reversible electrochemical response. This effect was attributed to the semiconducting nature of the PSi substrate and to the functional groups of the crosslinking molecules (MPTS), which together produce a capacitive effect on the device. On the other hand, the chemical modification of PSiMc with fluorescent ligands allowed us to fabricate fluorescent PSi hybrid nanostructures, which were tested for the detection of environmental pollutants such as heavy metals (specifically Hg 2+ ). We found that the selectivity of this optical device depends on the selected recognizing molecule. The captured metal induces the formation of a metallic complex that shows higher fluorescence compared with the sensor device. These results demonstrate the viability of using porous silicon as optical sensors and electrochemical biosensors. The infiltration of fluorescent recognizing molecules and proteins into the PSi matrix were evaluated by specular reflectance, FTIR spectroscopy, fluorescence spectroscopy and cyclic voltammetry.

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Section: Introductionmentioning

confidence: 98%

Porous Silicon Nanostructured Materials for Sensing Applications: Molecular Assembling and Electrochemical or Optical Evaluation

et al. 2016

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“…Porous silicon (PS)-based nanostructures are enjoying great relevance in developing label-free optical biosensors that can rapidly detect biological interactions between molecules [1][2][3][4]. The use of PS is motivated by the fact that its large available surface area allows numerous chemical molecules to attach receptors to PS surface, providing a greater number of capture probes to be locally accommodated into the PS, and hence increasing the molecular binding.…”

Section: Introductionmentioning

confidence: 99%

Study on the Formation of Au Nanoparticles on Fresh and Oxided Porous Silicon Microcavity Substrate

Zhang

Jia

Wang

2015

Integrated Ferroelectrics

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In this work, we study on the formation of Au nanoparticles separately on fresh and oxided porous silicon microcavity substrates. The fabrication of Au/PSM is simple, rapid, flexible and easy to be controlled. The amount of AuNPs deposited on fresh PSM substrates is much more than that of AuNPs deposited on oxided PSM substrates. And AuNPs are easy to be deposited on fresh PSM substrates. AuNPs deposited on fresh PSM substrate provide an interesting way in diverse areas of electronics, optics, material research, and medical science.

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“…Porous silicon (PS) has sponge-like structure, large internal surface-to-volume ratio [12][13], adjustable roughness and special structure, which can reduce the mismatch between ZnO nanostructures and Si substrates. It becomes a good substrate for ZnO nanostructure growing without using metal catalysts [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Application of porous silicon microcavity to enhance photoluminescence of ZnO/PS nanocomposites in UV light emission

Zhang

Jia

2017

Optik

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This paper presents ZnO nanostructures deposited on porous silicon (PS) and porous silicon microcavity (PSM) substrates by sol-gel method. The effects of annealing temperature and mass ration of zinc acetate to PVA on microstructure, and optical properties of ZnO/PS and ZnO/PSM nanocomposites were systematically investigated by field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) and photoluminescence (PL) spectroscopy. The XRD analysis confirmed that the ZnO/PS nanocomposite preferred strongly to (002) phase orientation. The PL spectra showed that there was a sharp and highly intense UV emission peak located at about 400 nm when annealing temperature was 800 o C for 30 min and the mass ratio of zinc acetate to PVA was 4:1. The detail cross-section FESEM images of ZnO/PSM showed that ZnO nanoparticles uniformly penetrated into all PS layers and adhered to them very well. Furthermore, ZnO/PSM nanocomposites showed relatively stronger UV emissions at around 400 nm compared to the PL spectra of ZnO/PS nanocomposites, that means one-dimension phonic crystal PSM can enhance the PL intensity of ZnO. Such a method may provide an interesting way to develop a new type of UV optoelectronic devices.

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Antifreeze protein detection using Rhodamine B as photoluminescence label in porous silicon

Cited by 15 publications

References 31 publications

Porous Silicon Nanostructured Materials for Sensing Applications: Molecular Assembling and Electrochemical or Optical Evaluation

Porous Silicon Nanostructured Materials for Sensing Applications: Molecular Assembling and Electrochemical or Optical Evaluation

Study on the Formation of Au Nanoparticles on Fresh and Oxided Porous Silicon Microcavity Substrate

Application of porous silicon microcavity to enhance photoluminescence of ZnO/PS nanocomposites in UV light emission

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