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Cervical biopsy (biopsy) is an important part of the diagnosis of cervical cancer. The artificial classification of biopsy images in diagnosis is difficult and depends on the clinical experience of pathologists. However, the classification accuracy of computerized biopsy tissue images with similar lesions is low, and the problem of incomplete experimental objects needs to be addressed. This paper proposes a method of cervical biopsy tissue image classification based on least absolute shrinkage and selection operator (LASSO) and ensemble learning-support vector machine (EL-SVM). Using the LASSO algorithm for feature selection, the average optimization time was reduced by 35.87 seconds while ensuring the accuracy of the classification, and then serial fusion was performed. The EL-SVM classifier was used to identify and classify 468 biopsy tissue images, and the receiver operating characteristic (ROC) curve and error curve were used to evaluate the generalization ability of the classifier. Experiments show that the normalcervical cancer classification accuracy reached 99.64%, the normal-low-grade squamous intraepithelial lesion (LSIL) classification accuracy was 84.25%, the normal-high-grade squamous intraepithelial lesion (HSIL) classification accuracy was 87.40%, the LSIL-HSIL classification accuracy was 76.34%, the LSILcervical cancer classification accuracy was 91.88%, and the HSIL-cervical cancer classification accuracy was 81.54%.

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In this study, an optical biosensor based on a porous silicon composite structure was fabricated using a simple method. This structure consists of a thin, porous silicon surface diffraction grating and a one-dimensional porous silicon photonic crystal. An angle-resolved diffraction efficiency spectrum was obtained by measuring the diffraction efficiency at a range of incident angles. The angle-resolved diffraction efficiency of the 2nd and 3rd orders was studied experimentally and theoretically. The device was sensitive to the change of refractive index in the presence of a biomolecule indicated by the shift of the diffraction efficiency spectrum. The sensitivity of this sensor was investigated through use of an 8 base pair antifreeze protein DNA hybridization. The shifts of the angle-resolved diffraction efficiency spectrum showed a relationship with the change of the refractive index, and the detection limit of the biosensor reached 41.7 nM. This optical device is highly sensitive, inexpensive, and simple to fabricate. Using shifts in diffraction efficiency spectrum to detect biological molecules has not yet been explored, so this study establishes a foundation for future work.

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Early detection of cervical lesions, accurate diagnosis of cervical lesions, and timely and effective therapy can effectively avoid the occurrence of cervical cancer or improve the survival rate of patients. In this paper, the spectra of tissue sections of cervical inflammation (n = 60), CIN (cervical intraepithelial neoplasia) I (n = 30), CIN II (n = 30), CIN III (n = 30), cervical squamous cell carcinoma (n = 30), and cervical adenocarcinoma (n = 30) were collected by a confocal Raman micro-spectrometer (LabRAM HR Evolution, Horiba France SAS, Villeneuve d’Ascq, France). The Raman spectra of six kinds of cervical tissues were analyzed, the dominant Raman peaks of different kinds of tissues were summarized, and the differences in chemical composition between the six tissue samples were compared. An independent sample t test (p ≤ 0.05) was used to analyze the difference of average relative intensity of Raman spectra of six types of cervical tissues. The difference of relative intensity of Raman spectra of six kinds of tissues can reflect the difference of biochemical components in six kinds of tissues and the characteristic of biochemical components in different kinds of tissues. The classification models of cervical inflammation, CIN I, CIN II, CIN III, cervical squamous cell carcinoma, and cervical adenocarcinoma were established by using a support vector machine (SVM) algorithm. Six types of cervical tissues were classified and identified with an overall diagnostic accuracy of 85.7%. This study laid a foundation for the application of Raman spectroscopy in the clinical diagnosis of cervical precancerous lesions and cervical cancer.