Antifouling and sensitive biosensor based on multifunctional peptide and urease@ZIFs for metal matrix protease-7

Anti‐Fouling Polymer or Peptide‐Modified Electrochemical Biosensors for Improved Biosensing in Complex Media

“…Peptide (EKEKEKE-PPPPC) on macroporous gold-coated GCE [133] Self-assembly DNA/Peptide (CTHNDRKQE)/PANI on PANI-coated GCE [134] Covalent attachment with PANI using succinimidyl 4-(N-maleimidomethyl)cyclohexane- (PABA)-coated GCE [135] Covalent attachment using carbodiimide/NHS AuNPs-coated GCE [140] Self-assembly DPV miRNA21 using aptamer hydrogel-coated GCE [141] Covalent attachment using carbodiimide/NHS SWV Matrix metalloproteinase 7…”

“…The flexibility inherent in the design of the peptides allows for the rational engineering of electrode coatings to address multiple detection challenges. For example, the negative charge [141] Copyright 2022, Elsevier.…”

Peptide-Based Hydrogels: New Materials for Biosensing and Biomedical Applications

“…Recently, Zhang et al fabricated a low-fouling electrochemical sensor demonstrating an accurate detection and capturing of tumor biomarkers in biological solutions. In this system, they used multifunctional peptides and zeolitic imidazolate frameworks (ZIFs) [ 69 ]. To prevent nonspecific attachment in biological media, they constructed a hydrophilic interface consisting of sodium alginate-graphene oxide-Pb 2+ (SA-GO-Pb 2+ ) gel and multipotent peptides (containing recognition, antifouling, and anchoring sequences).…”

Universal and High-Speed Zeptomolar Protein Serum Assay with Unprecedented Sensitivity

“…Protein biomarkers associated with the disease are often present in the serum at extremely trace levels that are difficult to monitor accurately. − Ultrasensitive and rapid clinical tests are crucial for early serum diagnosis and pathological studies. − The most commonly utilized approaches for protein analysis include enzyme-linked immunosorbent assays (ELISAs), , fluorescence resonance energy transfer (FRET), , surface-enhanced Raman scattering (SERS), , radioimmunoassay (RIA), , and electrochemistry (EC). , Notably, Walt et al pushed protein detection into the low attomolar range by individual counting with signal amplification on molecular beads using automatic flow cytometry . A major limitation of this approach is the need for sophisticated equipment and specialized operation during detection.…”

“…4−6 The most commonly utilized approaches for protein analysis include enzyme-linked immunosorbent assays (ELISAs), 7,8 fluorescence resonance energy transfer (FRET), 9,10 surface-enhanced Raman scattering (SERS), 11,12 radioimmunoassay (RIA), 13,14 and electrochemistry (EC). 15,16 Notably, Walt et al pushed protein detection into the low attomolar range by individual counting with signal amplification on molecular beads using automatic flow cytometry. 17 A major limitation of this approach is the need for sophisticated equipment and specialized operation during detection.…”