Peptide-Based Hydrogels: New Materials for Biosensing and Biomedical Applications

Binaymotlagh, Roya; Chronopoulou, Laura; Haghighi, Farid Hajareh; Fratoddi, Ilaria; Palocci, Cleofe

doi:10.3390/ma15175871

Cited by 50 publications

(51 citation statements)

References 167 publications

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“…The insurgence of resistance to multiple antimicrobial agents in pathogenic bacteria is already a major medical concern, compromising the clinical outcomes of many currently used drugs. By 2050, it is predicted that multidrug-resistant (MDR) pathogens will be causing more than 10 million deaths every year [ 1 , 2 , 3 , 4 , 5 , 6 ]. Unfortunately, the insurgence of MDR phenomena has overcome the rate of development of new antibiotics; therefore, exploring efficient or even alternative therapies seems urgent and necessary [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Green In Situ Synthesis of Silver Nanoparticles-Peptide Hydrogel Composites: Investigation of Their Antibacterial Activities

Binaymotlagh

Giudice

Mignardi

et al. 2022

Gels

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The present paper investigated the synthesis of peptide-based hydrogel composites containing photo-generated silver nanoparticles (AgNPs) obtained in the presence and absence of honey as tensile strength enhancer and hydrogel stabilizer. Fmoc-Phe and diphenylalanine (Phe2) were used as starting reagents for the hydrogelator synthesis via an enzymatic method. In particular, we developed an in situ one-pot approach for preparing AgNPs inside peptide hydrogels using a photochemical synthesis, without any toxic reducing agents, with reaction yields up to 30%. The structure and morphology of the nanohybrids were characterized with different techniques such as FESEM, UV-Vis, DLS, SAXS and XPS. Moreover, the antibacterial activity of these hybrid biomaterials was investigated on a laboratory strain and on a clinical isolate of Staphylococcus aureus. Results demonstrated that honey increased both swelling ability and also mechanical stability of the hydrogel. Finally, a higher antibacterial effect of AgNPs in the hybrid was observed in the presence of honey. In particular, AgNPs/hgel and AgNPs/hgel-honey showed an enhanced antibacterial activity (3.12 mg/L) compared to the free form of AgNPs, alone or in combination with honey (6.25 mg/L) for both S. aureus strains.

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Section: Introductionmentioning

confidence: 99%

Green In Situ Synthesis of Silver Nanoparticles-Peptide Hydrogel Composites: Investigation of Their Antibacterial Activities

Binaymotlagh

Giudice

Mignardi

et al. 2022

Gels

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“…Dipeptides are minimalistic peptides that can also form hydrogels through molecular self-assembly . Dipeptide-based hydrogels have been investigated as important tools in drug delivery, tissue regeneration, 3D cell culture, and imaging. − Dipeptide hydrogelators result from the molecular preorganizations and noncovalent interactions between peptide strands . Therefore, this new class of peptides is promiscuous for imparting shear thinning and self-healing properties in response to external stimuli.…”

Section: Introductionmentioning

confidence: 99%

Emerging Role of Injectable Dipeptide Hydrogels in Biomedical Applications

et al. 2023

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Owing to their properties such as biocompatibility, tunable mechanical properties, permeability toward oxygen, nutrients, and the ability to hold a significant amount of water, hydrogels have wide applications in biomedical research. They have been engaged in drug delivery systems, 3D cell culture, imaging, and extracellular matrix (ECM) mimetics. Injectable hydrogels represent a major subset of hydrogels possessing advantages of site-specific conformation with minimal invasive techniques. It preserves the inherent properties of drug/biomolecules and is devoid of any side effects associated with surgery. Various polymeric materials utilized in developing injectable hydrogels are associated with the limitations of toxicity, immunogenicity, tedious manufacturing processes, and lack of easy synthetic tunability. Peptides are an important class of biomaterials that have interesting properties such as biocompatibility, stimuli responsiveness, shear thinning, self-healing, and biosignaling. They lack immunogenicity and toxicity. Therefore, numerous peptide-based injectable hydrogels have been explored in the past, and a few of them have reached the market. In recent years, minimalistic dipeptides have shown their ability to form stable hydrogels through cooperative noncovalent interactions. In addition to inherent properties of lengthy peptide-based injectable hydrogels, dipeptides have the unique advantages of low production cost, high synthetic accessibility, and higher stability. Given the instances of expanding significance of injectable peptide hydrogels in biomedical research and an emerging recent trend of dipeptide-based injectable hydrogels, a timely review on dipeptide-based injectable hydrogels shall highlight various aspects of this interesting class of biomaterials. This concise review that focuses on the dipeptide injectable hydrogel may stimulate the current trends of research on this class of biomaterial to translate its significance as interesting products for biomedical applications.

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“…Peptide-derived self-assembled hydrogels have been particularly effective for drug delivery applications, including the delivery of protein payloads. − Koutsopoulos et al demonstrated that supramolecular hydrogels formulated from the self-assembled Ac-(RADA) 4 -NH 2 peptide facilitated the controlled release of lysozyme, trypsin inhibitor (TI), BSA, and immunoglobulin G (IgG) . They found that the rate of protein release from these neutral hydrogels decreased with increasing molecular weight of the payload, and that released proteins maintained functional catalytic/binding activity.…”

Section: Introductionmentioning

confidence: 99%

Supramolecular Phenylalanine-Derived Hydrogels for the Sustained Release of Functional Proteins

Jagrosse

Agredo

Abraham

et al. 2023

ACS Biomater. Sci. Eng.

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Protein-based therapeutics have emerged as nextgeneration pharmaceutical agents for oncology, bone regeneration, autoimmune disorders, viral infections, and other diseases. The clinical application of protein therapeutics has been impeded by pharmacokinetic and pharmacodynamic challenges including offtarget toxicity, rapid clearance, and drug stability. Strategies for the localized and sustained delivery of protein therapeutics have shown promise in addressing these challenges. Hydrogels are critical materials that enable these delivery strategies. Supramolecular hydrogels composed of self-assembled materials have demonstrated biocompatibility advantages over polymer hydrogels, with peptide and protein-based gels showing strong potential. However, cost is a significant drawback of peptide-based supramolecular hydrogels. Supramolecular hydrogels composed of inexpensive low-molecular-weight (LMW) gelators, including modified amino acid derivatives, have been reported as viable alternatives to peptide-based materials. Herein, we report the encapsulation and release of proteins from supramolecular hydrogels composed of perfluorinated fluorenylmethyloxcarbonyl-modified phenylalanine (Fmoc-F 5 -Phe-DAP). Specifically, we demonstrate release of four model proteins (ribonuclease A (RNase A), trypsin inhibitor (TI), bovine serum albumin (BSA), and human immunoglobulin G (IgG)) from these hydrogels. The emergent viscoelastic properties of these materials are characterized, and the functional and time-dependent release of proteins from the hydrogels is demonstrated. In addition, it is shown that the properties of the aqueous solution used for hydrogel formulation have a significant influence on the in vitro release profiles, as a function of the isoelectric point and molecular weight of the protein payloads. These studies collectively validate that this class of supramolecular LMW hydrogel possesses the requisite properties for the sustained and localized release of protein therapeutics.

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Peptide-Based Hydrogels: New Materials for Biosensing and Biomedical Applications

Cited by 50 publications

References 167 publications

Green In Situ Synthesis of Silver Nanoparticles-Peptide Hydrogel Composites: Investigation of Their Antibacterial Activities

Green In Situ Synthesis of Silver Nanoparticles-Peptide Hydrogel Composites: Investigation of Their Antibacterial Activities

Emerging Role of Injectable Dipeptide Hydrogels in Biomedical Applications

Supramolecular Phenylalanine-Derived Hydrogels for the Sustained Release of Functional Proteins

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