Antifatigue Activity of Liquid CulturedTricholoma matsutakeMycelium Partially via Regulation of Antioxidant Pathway in Mouse

Abstract

Tricholoma matsutake has been popular as food and biopharmaceutical materials in Asian countries for its various pharmacological activities. The present study aims to analyze the antifatigue effects on enhancing exercise performance of Tricholoma matsutake fruit body (ABM) and liquid cultured mycelia (TM) in mouse model. Two-week Tricholoma matsutake treatment significantly enhances the exercise performance in weight-loaded swimming, rotating rod, and forced running test. In TM- and ABM-treated mice, some factors were observed at 60 min after swimming compared with nontreated mice, such as the increased levels of adenosine triphosphate (ATP), antioxidative enzymes, and glycogen and the reduced levels of malondialdehyde and reactive oxygen species in muscle, liver, and/or serum. Further data obtained from western blot show that CM and ABM have strongly enhanced the activation of 5′-AMP-activated protein kinase (AMPK), and the expressions of peroxisome proliferator have activated receptor γ coactivator-1α (PGC-1α) and phosphofructokinase-1 (PFK-1) in liver. Our data suggest that both Tricholoma matsutake fruit body and liquid cultured mycelia possess antifatigue effects related to AMPK-linked antioxidative pathway. The information uncovered in our study may serve as a valuable resource for further identification and provide experimental evidence for clinical trials of Tricholoma matsutake as an effective agent against fatigue related diseases.

Anti-Ageing Effect of Physalis alkekengi Ethyl Acetate Layer on a d-galactose-Induced Mouse Model through the Reduction of Cellular Senescence and Oxidative Stress

“…The WLFST is used to evaluate the anti-fatigue effect of PAE, and the experiment was performed as described previously [55]. The mice were individually placed into the plastic pool (30 cm × 35 cm × 60 cm) containing freshwater (23 ± 1 • C), approximately 50 cm deep.…”

Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway

“…We have studied the pharmacological activities of TM for years and confirmed that TM possesses antifatigue effects related to the modulation of AMPK linked antioxidation pathway [25]. Recently, our data show that TM strongly enhances immunity of cyclophosphamide-induced immunosuppressed mouse models associated with nuclear factor kappa B signaling.…”

The anti‑fatigue activities of Tuber melanosporum in a mouse model

“…Fatigue can therefore be divided into two categories: Physical and mental fatigue. Physical fatigue is the inability of a muscle to maintain normal movement (2), which may induce a heightened stress response, endocrine dyscrasia, immune dysfunction and organ injury (3).…”

“…Various species of fungi and certain herbs may postpone the onset of fatigue, improve athletic ability and enhance the elimination of fatigue-related metabolites (13). The anti-fatigue effects of Tricholoma matsutake, Cordyceps militaris and Antrodia cinnamomea in murine models have been examined in previous studies by assessing their effect on oxidative stress (3,14,15). Tuber melanosporum (TM), a fungus containing unique bioactive metabolites, contains important nutrients including proteins, unsaturated fatty acids, amino acids, sphingolipids, cerebrosides and polysaccharides (16).…”