Antidiabetic Effects of Pediococcus acidilactici pA1c on HFD-Induced Mice

Abstract

Prediabetes (PreD), which is associated with impaired glucose tolerance and fasting blood glucose, is a potential risk factor for type 2 diabetes mellitus (T2D). Growing evidence suggests the role of the gastrointestinal microbiota in both PreD and T2D, which opens the possibility for a novel nutritional approach, based on probiotics, for improving glucose regulation and delaying disease progression of PreD to T2D. In this light, the present study aimed to assess the antidiabetic properties of Pediococcus acidilactici (pA1c) in a murine model of high-fat diet (HFD)-induced T2D. For that purpose, C57BL/6 mice were given HFD enriched with either probiotic (1 × 1010 CFU/day) or placebo for 12 weeks. We determined body weight, fasting blood glucose, glucose tolerance, HOMA-IR and HOMA-β index, C-peptide, GLP-1, leptin, and lipid profile. We also measured hepatic gene expression (G6P, PEPCK, GCK, IL-1β, and IL-6) and examined pancreatic and intestinal histology (% of GLP-1+ cells, % of goblet cells and villus length). We found that pA1c supplementation significantly attenuated body weight gain, mitigated glucose dysregulation by reducing fasting blood glucose levels, glucose tolerance test, leptin levels, and insulin resistance, increased C-peptide and GLP-1 levels, enhanced pancreatic function, and improved intestinal histology. These findings indicate that pA1c improved HFD-induced T2D derived insulin resistance and intestinal histology, as well as protected from body weight increase. Together, our study proposes that pA1c may be a promising new dietary management strategy to improve metabolic disorders in PreD and T2D.

Pediococcus acidilactici CECT9879 (pA1c) Counteracts the Effect of a High-Glucose Exposure in C. elegans by Affecting the Insulin Signaling Pathway (IIS)

“…Hence, we can hypothesize that pA1c activity needs not only the daf-16 effect, but all the insulin signaling pathway (IIS). These findings are consistent and support the data obtained from the study of pA1c supplementation in mice [ 22 ], in which it was seen that 12-week pA1c supplementation significantly attenuated body weight gain, mitigated glucose dysregulation by reducing fasting blood glucose levels, glucose tolerance test, leptin levels, and insulin resistance.…”

“…Previous studies in mice fed high fat and high sugar diets have demonstrated that Pediococcus acidilactici CECT9879 (pA1c) has beneficial properties on glucose homeostasis including an anti-hyperglycemic effects [ 22 ]. In this study, we have evaluated the metabolic activities of the probiotic strain Pediococcus acidilactici CECT9879 (pA1c) on C. elegans lipid accumulation, insulin/IGF-1 signaling (IIS), health span, oxidative stress, and aging, and its response to a high-glucose exposure.…”

Effects of probiotic Lactiplantibacillus plantarum IMC 510 supplementation on metabolic factors in otherwise healthy overweight and obese individuals

“…plantarum IMC 510 can improve the blood parameters and suggest that its benefits are possibly limited in subjects showing haematologic alterations. Notably, a large number of studies showed that certain probiotics may be able to lower glycaemia and serum lipids, particularly in diabetic or hypercholesterolemic subjects in clinical (Jones et al, 2012; Khalili et al, 2022; Kocsis et al, 2020; Wang et al, 2018; Wu et al, 2017; Zhang et al, 2022) and in pre‐clinical research (Cabello‐Olmo et al, 2022; Marques et al, 2020; Ooi & Liong, 2010; Reamtong et al, 2021; Wang et al, 2012). Notably, last year, three probiotics were tested in a healthy rabbit (see details in Kadja et al, 2021) and improved the biochemical and haematological profiles.…”

The effects of probiotic administration on patients with prediabetes: a meta-analysis and systematic review

“…Bifidobacterium longum and Lactobacillus reuteri could increase mucus layer thickness [ 56 , 57 ]. Pediococcus acidilactici pA1c increases the number of cupped cells, which promote the secretion of mucus glycoproteins and maintain an appropriate length of intestinal villi [ 58 ]. Oxidative stress may play a role in damaging glucose metabolism by impairing pancreatic β cells and insulin signalling pathways.…”