Antidiabetic Activity of Extracts of Pithecellobium Dulce Benth Leaves in Alloxan Induced Diabetic Rats

Abstract

The objective of the present study was to study effect of Pithecellobium dulce Benth (P. dulce) leaves in alloxan induced diabetic rats. The P. dulce leaves were extracted by maceration and soxhelation method by using water and ethanol as solvent. Acute toxicity study was performed according to OECD 425 guidelines for both aqueous and ethanolic extracts of P. dulce leaves. The dose of 200 mg/kg and 400 mg/kg was selected for further studies. Animals were rendered diabetic by administration of alloxan (130 mg/kg, i.p.). The albino rats were divided in to seven groups with five animals in each group. Diabetic animals were treated with aqueous and ethanolic extract for 20 days. Then blood glucose, triglyceride, total cholesterol, urea, uric acid, creatinine, aspartate aminotransferase (AST), alanine transaminase (ALT) and glycogen level in liver, muscle and kidney were estimated according to standard procedures. The result shows significant decrease in blood glucose, triglyceride, total cholesterol, urea, uric acid, creatinine, AST and ALT level when compared to diabetic group. The liver and muscle glycogen level was increased significantly in extract treated groups when compared to diabetic control group. Both extract of P. dulce posses antidiabetic and hypolipidemic potential.

Amelioration of hyperglycaemia and modulation of antioxidant status by Alcea rosea seeds in alloxan-induced diabetic rats

“…2003 ; Mule et al. 2016 ). After 72 h, animals with fasting blood glucose levels of >200 mg/dL were considered diabetic and selected for further experimentation.…”

Traditional fruits of South India: Bioactive components and their potential health implications in chronic diseases

“…Another study was performed in alloxan-induced diabetic rats to evaluate the lipid lowering effects of aqueous and ethanolic leaf extract of P. dulce. It was confirmed that the increased serum cholesterol, TG, and decreased high-density lipoproteins (HDL) cholesterol levels observed in alloxan-treated rats were found to be reversed upon treatment with aqueous and ethanolic leaf extract of P. dulce (Mule, Naikwade, Magdum, & Jagtap, 2016a).…”

“…Another study was performed in alloxan‐induced diabetic rats to evaluate the lipid lowering effects of aqueous and ethanolic leaf extract of P. dulce . It was confirmed that the increased serum cholesterol, TG, and decreased high‐density lipoproteins (HDL) cholesterol levels observed in alloxan‐treated rats were found to be reversed upon treatment with aqueous and ethanolic leaf extract of P. dulce (Mule, Naikwade, Magdum, & Jagtap, 2016a). In addition, increased total cholesterol and TG observed in the serum of dexamethasone‐induced diabetic rats was found to decrease upon treatment with aqueous and ethanolic leaf extract of P. dulce (Mule et al., 2016b).…”

“…Studies has been confirmed that the presence of steroids, saponins, lipids, phospholipids, glycosides, glycolipids, and polysaccharides have been reported in the seeds and bark contains tannins of catechol type and quericitin, kaempferol, dulcitol, and afezilin have been found in the leaves (Kumar, Nehra, & Duhan, 2013). Because of the above mentioned phytochemical constituents it has been reported to be a folk remedy for diabetes, cardiovascular disease, inflammation, oxidative stress, bacterial, viral, and fungal infection (Mule, Naikwade, Magdum, & Jagtap, 2016b; Pal, Pal, Manna, & Sil, 2012; Toudji et al., 2018). In addition, it has been reported to be used as astringent in dysentery, febrifuge, dermatitis, and eye inflammation (Selvan & Muthukumaran, 2011).…”

“…This showed P. dulce leaf extract exhibited remarkable glucose lowering effect in alloxan‐treated rats. In addition, aqueous and ethanolic leaf extract of P. dulce significant antidiabetic activity by lowering blood glucose level in dexamethasone‐induced diabetic rats (Mule et al., 2016b).…”