Antidiabetic Activities of Broccoli Extracts (Brassica oleracea L.var italica) on Mice Induced Streptozotocin-Nicotinamide

Abstract

Several plant species have been used for the treatment of diabetes. Research on plants that can be used to treat diabetes such as Cinnamomum cassia and Cinnamomum zeylanicum, Piper sarmentosum Roxb, Nelumbo nucifera, Ceiba petandra, Memecylon malabaricum. Broccoli (Brassica oleracea L var italics) is a flower of vegetables like cabbage plants. Broccoli juice is made capable of direct and indirect effects on the reduction of blood LDL; the indirect effect is by repairing pancreatic beta cells and increasing insulin sensitivity. The purpose of this study was to determine whether broccoli has activity as antidiabetic. The subjects consisted of 15 mice induced with nicotinamide 70 mg/kg BW and Stz 150 mg/kg BW. Diabetic mice were divided into the positive control group (glibenclamide), negative control group (CMC Na) and three treatment groups. The test material is broccoli extract dose 75 mg/kg BW, 150 mg /kg BW and 300 mg/kg BW given peroral for 14 days. Anova test results showed that there was a significant difference in the average percentage of decreased blood glucose levels in all treatments. The result of Post Hock Tukey HSD test with a 95% confidence level showed a significant difference in the negative control group compared with positive control group and extract 300 mg/kg BW. This result shows that positive control group and extracts 300 mg/kg body weight have an activity to decrease blood glucose level.

UHPLC-ESI-QTOF-MS/MS Metabolite Profiling of the Antioxidant and Antidiabetic Activities of Red Cabbage and Broccoli Seeds and Sprouts

“…Maeda et al also reported similar results for the broccoli sprouts with higher sulforaphane contents [36]. The phytochemicals, including alkaloids, flavonoids, saponins, and steroids, are responsible for the antidiabetic activities in brassica vegetables [37].…”

Isolation of bioactive peptides and multiple nutraceuticals of antidiabetic and antioxidant functionalities through sprouting: Recent advances

“…Lutfiyati et al (2018) studied the effect of broccoli leaf methanolic extract on the lowering of blood sugar for diabetic mice. The mice were induced with diabetes using streptozotocin 150 mg/kg BW and nicotinamide 70 mg/mg BW, administered perorally with the extracts at concentrations of 75, 150, and 300 mg/kg BW for 14 days, and their blood sugar subsequently examined.…”

“…The serum insulin concentration and fasting serum glucose were found to significantly decrease with the 10 grams per day dosage having the largest decrease, especially for the IR index (as HOMA-IR).López-Chillón et al (2019) examined the effect of simulated gastrointestinal digestion on the alpha-glucosidase inhibitory activity of broccoli sprout extracts. The study was contrived to confirm the antidiabetic potential subsequent to digestion, and the inhibition of the digestive enzyme was found to decrease from 7.88 ± 0.66% to 3.60 ± 1.20% indicating a positive tendency of the sprout to be biofunctional after its subjection to digestive conditions Lutfiyati et al (2018). studied the effect of broccoli leaf methanolic extract on the lowering of blood sugar for diabetic mice.…”

Literature Review: Antidiabetic Activities and Phytochemical Screening of Broccoli Plants (Brassica oleracea L. Var. italica)

“…Research on the antidiabetic effect of broccoli has been carried out by [17] using broccoli ethanol extract samples. In this study, experimental animals were used in the form of rats that were induced with Streptozotocin-Nicotinamide until blood sugar levels were > 100 mg/dL.…”