Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes

Truta, Liliana A.A.N.A.; Castro, André L.; Tarelho, Sónia; Costa, Pedro Reis; Sales, M. Goreti F.; Teixeira, Helena M.

doi:10.1016/j.jpba.2016.06.027

Cited by 48 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Working with embryos and larvae requires the setup of extremely sensitive detection methods, due to the very small sample size and the high lipid content. Solid-phase extraction (SPE) and liquid-liquid extraction (LLE) are widely used for the extraction of SSRIs from biological and tissue samples [29][30][31][32][33]. Usually, SPE has better reproducibility than LLE methods; however, they are also complex and time-consuming and require large amounts of solvents.…”

Section: Introductionmentioning

confidence: 99%

Development of a method for assessing the accumulation and metabolization of antidepressant drugs in zebrafish (Danio rerio) eleutheroembryos

et al. 2021

View full text Add to dashboard Cite

Antidepressant drugs are widely used for the treatment of common mental or other psychiatric disorders such as depression, which affect about 121 million people worldwide. This widespread use has contributed to the input of these pharmaceuticals and their metabolites into the environment. The aim of this work was to develop an analytical method to quantify the most widely used antidepressant drugs, selective serotonin reuptake inhibitors (SSRI), and their main metabolites in the environment. For this, a new and reliable miniaturized extraction method based on dispersive SPE cleanup procedure for extraction of SSRI followed by derivatization with n-heptafluorobutyrylimidazole, and detection by GC-MS was developed. The methodology, including a first-order one-compartment model, was then applied to a bioconcentration study in zebrafish (Danio rerio) eleutheroembryos. The results showed low bioaccumulation of these compounds; however, a biotransformation evidence of the parent compounds into their metabolites was observed after 6 h of exposure. These results indicate the need to integrate metabolic transformation rates to fully model and understand the bioaccumulation patterns of SSRI and their metabolites.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of a method for assessing the accumulation and metabolization of antidepressant drugs in zebrafish (Danio rerio) eleutheroembryos

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Therefore, the development of analytical methods to determine the residues of ADs in human samples is very important in areas such as medicine and forensics. Several analytical techniques can be employed for these purposes, such as gas and liquid chromatography, capillary electrophoresis, and spectrophotometry, among others [1,[9][10][11]. Considering the current demand for methods to be more sensitive and selective, the coupling between liquid chromatography and mass spectrometry becomes a useful way to improve the analytical results even more.…”

Section: Introductionmentioning

confidence: 99%

Multidimensional Liquid Chromatography Employing a Graphene Oxide Capillary Column as the First Dimension: Determination of Antidepressant and Antiepileptic Drugs in Urine

et al. 2020

View full text Add to dashboard Cite

Human mental disorders can be currently classified as one of the most relevant health topics. Including in this are depression and anxiety, which can affect us at any stage of life, causing economic and social problems. The treatments involve cognitive psychotherapy, and mainly the oral intake of pharmaceutical antidepressants. Therefore, the development of analytical methods for monitoring the levels of these drugs in biological fluids is critical. Considering the current demand for sensitive and automated analytical methods, the coupling between liquid chromatography and mass spectrometry, combined with suitable sample preparation, becomes a useful way to improve the analytical results even more. Herein we present an automated multidimensional method based on high-performance liquid chromatography-tandem mass spectrometry using a lab-made, graphene-based capillary extraction column connected to a C8 analytical column to determined five pharmaceutical drugs in urine. A method enhancement was performed by considering the chromatographic separation and the variables of the loading phase, loading time, loading flow, and injection volume. Under optimized conditions, the study reports good linearity with R2 > 0.98, and limits of detection in the range of 0.5–20 µg L−1. Afterward, the method was applied to the direct analysis of ten untreated urine samples, reporting traces of citalopram in one of them. The results suggest that the proposed approach could be a promising alternative that provides direct and fully automated analysis of pharmaceutical drugs in complex biological matrices.

show abstract

“…Due to its widespread use, there is a need to develop a simple, easy, and rapid method for assessing the quality of fluoxetine in pharmaceutical preparation and biological fluids. In this regard, several methods have been proposed in past to analyze fluoxetine in pharmaceutical formulation, spiked urine and blood serum samples including capillary electrophoresis [6,7], HPLC [8][9][10][11][12], voltammetry [13], spectrophotometry [14][15][16] and gas chromatography [17]. No doubt, most of these methods are highly sensitive but they require tedious procedures and expensive chemicals.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric detection of fluoxetine using citrate-capped silver nanoparticles

Laghari

Khuhawar

2020

SN Appl. Sci.

View full text Add to dashboard Cite

A novel colorimetric assay has been presented for the detection of fluoxetine using citrate-capped silver nanoparticles (CIT-Ag NPs) as a colorimetric sensor. The detection of fluoxetine was accomplished by observing color change of CIT-Ag NPs solution from yellow to dark brown due to aggregation induced by fluoxetine. The aggregation of CIT-Ag NPs was characterized by various techniques including UV-Vis spectrometry, scanning electron microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential. A characteristic absorption peak of CIT-Ag NPs at 400 nm was red shifted and surface plasmon resonance band became broad due to addition of fluoxetine. Under optimized conditions, linear calibration curve was obtained within concentration range of 2-10 μg/ml with detection limit of 0.18 µg/ml. Furthermore, the developed colorimetric assay was found to be simple, sensitive, reproducible, and selective towards a number of pharmaceutical additives. It involved sensing of fluoxetine by naked eye within few minutes without requiring any complicated instrumentation. Colorimetric platform was applied to detect fluoxetine in pharmaceutical formulation, and biological fluids including spiked human urine and blood serum samples.

show abstract

Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes

Cited by 48 publications

References 22 publications

Development of a method for assessing the accumulation and metabolization of antidepressant drugs in zebrafish (Danio rerio) eleutheroembryos

Development of a method for assessing the accumulation and metabolization of antidepressant drugs in zebrafish (Danio rerio) eleutheroembryos

Multidimensional Liquid Chromatography Employing a Graphene Oxide Capillary Column as the First Dimension: Determination of Antidepressant and Antiepileptic Drugs in Urine

Colorimetric detection of fluoxetine using citrate-capped silver nanoparticles

Contact Info

Product

Resources

About