Anticoagulant activity of low-molecular-weight sulfated derivatives of galactomannan from Cyamopsis tetragonoloba (L.) seeds

Are Structurally Modified Galactomannan Derivatives Biologically Active?

“…(guar gum) underwent enzymatic hydrolysis, followed by sulfation of fractions with different molecular weights. All sulfated derivatives exhibited anticoagulant activity [9]. That study also demonstrated that the mechanism of inhibition of blood plasma coagulation factors by galactomannan sulfate differed from the mechanism of inhibition by heparin.…”

“…Sulfation is the most studied chemical modification in galactomannans. Most studies about sulfation of galactomannans used dimethylformamide as the solvent and SO 3 pyridine complex as a sulfating reagent [6,8,9,23,[26][27][28][29]. Several studies report preferred sulfation for the 6-CH 2 groups due to their higher reactivity with esterifying agents and a degree of sulfating (DS) ranging from 0.4 to 1.85.…”

“…Several studies report preferred sulfation for the 6-CH 2 groups due to their higher reactivity with esterifying agents and a degree of sulfating (DS) ranging from 0.4 to 1.85. These sulfated galactomannans are composed of negatively charged sulfate groups that are related to their biological activities [4][5][6]8,9,23,[26][27][28][29]. The addition of sulfate groups in the polysaccharide structure improves solubility and enhances the biological properties compared to the non-sulfated polysaccharides.…”

“…Among the plant-derived polysaccharides, galactomannans have received much attention, since these biopolymers can be obtained in their pure form, with a high yield, by aqueous extraction from the endosperm of the seeds of leguminous plants [6,8]. Furthermore, since galactomannans are an abundant plant resource, their production on an industrial scale has been widely targeted [5,9], especially in the food, pharmaceutical, and cosmetic industries [10].…”

Sulfation modification and anticoagulant activity of the polysaccharides obtained from persimmon (Diospyros kaki L.) fruits

“…Pires et al reported that the active fraction from sulfated galactomannan possessed further prolonged coagulation time and its activity was similar to that of heparin [7]. Also, the effects of sulfation on anticoagulant activity for chitosan, galactan, and glucan were reported [8,9]. These earlier results demonstrated that the anticoagulant effect of sulfated polymers mainly depended on various structure factors of polymers including the degree and pattern of sulfation, molecular weight and glycosidic linkage [10].…”