Anticancer therapeutic potential of phosphorylated galactosylated chitosan against N-nitrosodiethyl amine-induced hepatocarcinogenesis

Anushree, U; Shetty, Shricharith; Kulkarni, Suresh D.; B, Harish Kumar; Pai, K. Sreedhara Ranganath; Aranjani, Jesil Mathew; Kumar, Rajesh; Bharati, Sanjay

doi:10.1016/j.abb.2022.109375

Cited by 8 publications

(2 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CS and its derivatives can make the pH of body fluid tend to be weak and alkaline, thereby improving the activity of lymphocytes, enhancing the activity of lymphocytes to destroy cancer cells, and playing a role in cancer prevention [130]. Anushree et al [131] evaluated that phosphorylated galactose-CS (PGC) had good metal chelation, iron ion reduction, and free radical scavenging activity. The number of tumor-free animals in the PGC treatment group increased, and the tumor diversity decreased significantly.…”

Section: Application Of Cs In Functional Foodsmentioning

confidence: 99%

“…Studies have demonstrated that CS and its derivatives have good metal chelation, iron ion reduction, and free radical scavenging activities. They have high antioxidant activity and anti-proliferation effects on cancer cells but have no cytotoxicity on normal cells, which shows the potential of anticancer therapy [131,132]. Some studies have shown that CS and its derivatives regulate lipid metabolism and glucose metabolism in the body, reduce intestinal absorption of lipids, and have lipid-lowering and anti-obesity effects [128].…”

Section: Application Of Cs In Functional Foodsmentioning

confidence: 99%

See 1 more Smart Citation

Harnessing the Potential of Chitosan and Its Derivatives for Enhanced Functionalities in Food Applications

Yi,

Miao,

Yang

et al. 2024

Foods

View full text Add to dashboard Cite

As one of the most abundant natural polysaccharides that possess good biological activity, chitosan is extracted from chitin. Its application in the food field is being increasingly valued. However, chitosan extraction is difficult, and its poor solubility limits its application. At present, the extraction methods include the acid–base method, new chemical methods, and biological methods. The extraction rates of chitin/chitosan are 4–55%, 13–14%, and 15–28%, respectively. Different chemical modifications have different effects on chitosan, making it applicable in different fields. This article reviews and compares the extraction and chemical modification methods of chitosan, emphasizing the importance of green extraction methods. Finally, the application prospects of chitosan in the food industry are discussed. This will promote the understanding of the advantages and disadvantages of different extraction methods for chitosan as well as the relationship between modification and application, providing valuable insights for the future development of chitosan.

show abstract

Section: Application Of Cs In Functional Foodsmentioning

confidence: 99%

Section: Application Of Cs In Functional Foodsmentioning

confidence: 99%

Harnessing the Potential of Chitosan and Its Derivatives for Enhanced Functionalities in Food Applications

Yi,

Miao,

Yang

et al. 2024

Foods

View full text Add to dashboard Cite

show abstract

Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats

et al. 2022

Self Cite

View full text Add to dashboard Cite

Phosphorylated chitosan (PC), a water-soluble derivative of chitosan possesses several biological and chemical properties suitable for diabetic wound healing. In the present study, we report the synthesis and diabetic wound healing capabilities of PC. Elemental analysis, FT-IR, 13C-NMR and 31P-NMR techniques were employed for the chemical characterization of PC. In vitro, antioxidant properties of PC were determined in terms of Fe3+ reducing, metal chelating, lipid peroxidation and superoxide scavenging ability. The wound healing potential of PC was assessed in diabetic excisional wound rat model. PC exhibited good water solubility, and in vitro antioxidant capacity. Wound contraction was higher in PC-treated wounds (91.11%) as compared to untreated wounds (67.26%) on 14th-day post wound creation. Histopathology of PC-treated wounds revealed improved tissue morphology with higher number of fibroblasts, a thicker epithelial layer, enhanced collagen deposits and angiogenesis as compared to untreated wounds. An overall increase of 57% and 25% in hydroxylamine and hexosamine content respectively were noted as compared to untreated wounds. A significant (P ≤ 0.05) increase in SOD activity and a significant (P ≤ 0.05) decrease in lipid peroxides were recorded in PC-treated wounds as compared to untreated wounds. These observations demonstrated that PC can be used as an effective agent in diabetic wound healing. Graphical Abstract Illustration of phosphorylated chitosan (PC) synthesis and its wound healing potential: Chitosan was phosphorylated to impart diabetic wound healing properties. Chemical characterizations such as elemental analysis, FT-IR and NMR confirmed successful phosphorylation of chitosan. PC exhibited good in vitro antioxidant properties. To assess the diabetic wound healing potential, an excisional wound model was developed in diabetic rats. PC treatment demonstrated accelerated wound healing.

show abstract

Anticancer therapeutic potential of multimodal targeting agent- “phosphorylated galactosylated chitosan coated magnetic nanoparticles” against N-nitrosodiethylamine-induced hepatocellular carcinoma

Udupi,

Shetty,

Aranjani

et al. 2024

Drug Deliv. and Transl. Res.

View full text Add to dashboard Cite

Superparamagnetic iron oxide nanoparticles (SPIONs) are extensively used as carriers in targeted drug delivery and has several advantages in the field of magnetic hyperthermia, chemodynamic therapy and magnet assisted radionuclide therapy. The characteristics of SPIONs can be tailored to deliver drugs into tumor via “passive targeting” and they can also be coated with tissue-specific agents to enhance tumor uptake via “active targeting”. In our earlier studies, we developed HCC specific targeting agent- “phosphorylated galactosylated chitosan”(PGC) for targeting asialoglycoprotein receptors. Considering their encouraging results, in this study we developed a multifunctional targeting system- “phosphorylated galactosylated chitosan-coated magnetic nanoparticles”(PGCMNPs) for targeting HCC. PGCMNPs were synthesized by co-precipitation method and characterized by DLS, XRD, TEM, VSM, elemental analysis and FT-IR spectroscopy. PGCMNPs were evaluated for in vitro antioxidant properties, uptake in HepG2 cells, biodistribution, in vivo toxicity and were also evaluated for anticancer therapeutic potential against NDEA-induced HCC in mice model in terms of tumor status, electrical properties, antioxidant defense status and apoptosis. The characterization studies confirmed successful formation of PGCMNPs with superparamagnetic properties. The internalization studies demonstrated (99–100)% uptake of PGCMNPs in HepG2 cells. These results were also supported by biodistribution studies in which increased iron content (296%) was noted inside the hepatocytes. Further, PGCMNPs exhibited no in vivo toxicity. The anticancer therapeutic potential was evident from observation that PGCMNPs treatment decreased tumor bearing animals (41.6%) and significantly (p ≤ 0.05) lowered tumor multiplicity. Overall, this study indicated that PGCMNPs with improved properties are efficiently taken-up by hepatoma cells and has therapeutic potential against HCC. Further, this agent can be tagged with 32P and hence can offer multimodal cancer treatment options via radiation ablation as well as magnetic hyperthermia. Graphical Abstract Schematic illustration of PGCMNPs synthesis, characterization and its anticancer potential: PGCMNPs were synthesized by co-precipitation method. The successful synthesis of PGCMNPs was confirmed by physical and chemical characterizations. PGCMNPs were biocompatible and exhibited no toxicity at tested parameters. PGCMNPs demonstrated higher uptake in HepG2 cells. The anticancer therapeutic potential of PGCMNPs in HCC mouse model, was evident from improved tumor statistics, increased low conductivity tumors and increased apoptosis mediated cell death.

show abstract

Anticancer therapeutic potential of phosphorylated galactosylated chitosan against N-nitrosodiethyl amine-induced hepatocarcinogenesis

Cited by 8 publications

References 76 publications

Harnessing the Potential of Chitosan and Its Derivatives for Enhanced Functionalities in Food Applications

Harnessing the Potential of Chitosan and Its Derivatives for Enhanced Functionalities in Food Applications

Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats

Anticancer therapeutic potential of multimodal targeting agent- “phosphorylated galactosylated chitosan coated magnetic nanoparticles” against N-nitrosodiethylamine-induced hepatocellular carcinoma

Contact Info

Product

Resources

About