Anticancer Constituents and Cytotoxic Activity of Methanol-Water Extract of <em>Polygonum Bistorta</em> L.

Abstract

This study was specifically designed to identify anticancer constituents in methanol-water extract of Polygonum bistorta L. and evaluate its cytotoxicity. For this purpose methanol-water (40:60 v/v) extract was subjected to conventional preparative high pressure liquid chromatography and 13 fractions were obtained. Constituents of obtained fractions were separated and identified with the help of GC-MS and LC-DAD-ESI-MS. Anticancer phenolic compounds such as gallic acid, protocatechuic acid, p-hydroxybenzoic acid, chlorogenic acid, vanillic acid, syringic acid, catechol, 4-methyl catechol, syringol and pyrogallol and fatty acids such as linoleic acid, myristic acid and palmitic acid were separated from different fractions. Fractions were evaluated for their cytotoxic activity on a rarely studied human hepatocellular carcinoma cell line (HCCLM3). 11 fractions showed good to strong cytotoxicity in a range of 200 µg/mL-800 µg/mL, whereas 2 fractions did not show any activity even at 800 µg/mL and no anticancer constituent was detected from them. 50 percent growth inhibition (GI 50 ) values for five most active fractions were calculated and results were in a range of 86.5 (±3) µg/mL-126.8 (±3) µg/mL. 3 out of these 5 most active fractions were found to contain phenolic content in them whereas all other fractions containing phenolic content did possess cytotoxic activity that may suggest the importance of phenolic constituents in anticancer activity. Moreover, the results also showed a definite dose dependent relationship between amount of fractions and cytotoxic activity.

Composition, Antifungal and Antiproliferative Activities of the Hydrodistilled Oils from Leaves and Flower Heads of Pterocephalus nestorianus Nábělek

“…(8) 78.04 (7) 84.92 (4) Aldehydes (13) 3.14 (10) 3.39 (12) Ketones (6) 3.2 (4) 1.01 (5) Alcohols (7) 1.03 (5) 4.1 (6) Esters (5) 1.7 (3) 1.2 (5) Others (7) 0.55 (5) 2.77 (7) Because of the heterogenous compositions of PFO and PLO, it is hard to identify the components responsible for such high antiproliferative activity. [11] [12] In particular, lauric acid induced apoptosis in Caco-2 and IEC-6 cell lines, due to reduced levels of antioxidant GSH, concomitant with a reduction of cells in the G0/G1 phase and an increased ROS generation. [10] However, terpenoids are minor components in PLO which, compared to PFO, is equally active or even more effective against some tumor cell lines (Table 3).…”

“…In fact, a significant antiproliferative activity has been determined for both acids. [11] [12] In particular, lauric acid induced apoptosis in Caco-2 and IEC-6 cell lines, due to reduced levels of antioxidant GSH, concomitant with a reduction of cells in the G0/G1 phase and an increased ROS generation. [12] To explore this hypothesis, lauric and myristic acids were tested against the same tumor cell lines ( Table 3).…”

Gas Chromatography-Mass Spectrometry Analysis, in Vitro Cytotoxic and Antioxidant Efficacy Studies on Cleome Gynandra L. (Leaves): A Traditional Drug Source

“…It has been previously reported that phytol, an acyclic diterpene alcohol, plays a key role in the control of tumor growth by reducing the level of cytokine production and also by preventing the formation of free radicals [29]. Phytol also exhibits antimicrobial, anticancer, diuretic, and anti-inflammatory [30].…”

Advanced analytical techniques for the extraction and characterization of plant-derived essential oils by gas chromatography with mass spectrometry

“…Intisar et al has isolated EOs of Polygonum bistorta L. with HD mode of extraction. Further analyses of the active constituents of EOs have proved that EOs have anticancer activities when used with solvent extracts .…”