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Objective: The objective of the study was to determine the anticancer efficacy of Orthosiphon stamineus extract against Michigan Cancer Foundation-7 (MCF-7) and its phytochemical analysis through gas chromatography-mass spectrometry (GC-MS).Methods: Different solvents were used for leaf extraction and used for qualitative assay of phytochemicals using standard protocols. Different concentration (12.5, 25, 50, 100, and 200 µg/ml) of methanol extract and ethyl acetate extract of O. stamineus leaves were used to assess the in vitro cytotoxic effect using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Further, the ethyl acetate extract was subjected to GC-MS analysis, and the identification of components was based on the National Institute of Standards and Technology library database. Result:Of the hexane, methanol and ethyl acetate extracts, methanol extract found to contain more of phytochemicals followed by ethyl acetate. The inhibitory concentration 50 for methanol extract and ethyl acetate extract was found be 93.42 µg/ml and 215.3 µg/ml, respectively.GC-MS mass spectrum of ethyl acetate extract revealed the presence of squalene and phytol and antioxidants such as flavones. Sterols compounds such as vitamin E, ergosterol, cholesterol, and 3,7,11,15-tetramethyl-2-hexadecen-1-ol and terpenoids were also identified. Conclusion:The data obtained in this work could be useful as a chemical standard in checking the genuineness of this plant source. Data of the results further depicted that the selected traditional anti-cancer plants could be used not only as an anti-cancer but also as a good antioxidant.

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<p>The aim of the present study was to analyze the anti-cancer effect of <em>Orthosiphon stamineus</em> leaf extract on MCF-7 cells. Ethyl acetate extract of the plant was eluted and pooled into 6 fractions based on their R_f values and the fractions were tested for cytotoxicity. Fraction 3 was found effective (IC₅₀= 28.5 µL/mL) and was subjected to LC-MS analysis which indicated the presence of biochanin, eleutherol and cinnamic acid. Further, cytotoxic effect of the fraction was confirmed in terms of nucleolin, bcl₂, bax and cytochrome c expression in MCF-7 cells through RT-PCR where a marked decrease in nucleolin, bcl₂ and increase in bax and cytochrome c were observed. The presence of eleutherol, a well-known anti-oxidant and anti-cancer agent, has been reported for the first time in<em> O. stamineus</em>. The anti-cancer activity of <em>O. stamineus</em> may be due to the presence of elutherol and cinnamic acid.</p><p><strong>Video Clip of Methodology</strong>:</p><p>LC-MS analysis of <em>Orthosiphon stamineus</em> extract: 7 min 33 sec   <a href="https://youtube.com/v/7Vop4epRjB0">Full Screen</a>   <a href="https://youtube.com/watch?v=7Vop4epRjB0">Alternate</a> </p>

The octadecanoic acid (OA), methyl ester was isolated and characterized from root of fenugreek (T. foenumgraecum) using ethyl acetate (EAC) in hydroponics method. The OA was characterized by TLC & HPTLC and purified by HPLC. The structure and molecular weight (298) of OA was confirmed by GC-MS. The R f value of sample (peak 4) was matched with standard. OA has strong anti-bacterial activity in B. subitlis and P. aeruginousa, where as weak activity in S. aureus and E. coli. Among the tested concentration of in vitro anti-oxidant activity of OA was maximum in 40μl and minimum in 20μl.