Anticancer and apoptosis-inducing effects of quercetin in vitro and in vivo

Hashemzaei, Mahmoud; Far, Amin Delarami; Yari, Arezoo; Heravi, Reza Entezari; Tabrizian, Kaveh; Taghdisi, Seyed Mohammad; Sadegh, Sarvenaz Ekhtiari; Tsarouhas, Konstantinos; Kouretas, Demetrios; Tzanakakis, George N.; Nikitovic, Dragana; Анисимов, Никита; Spandidos, Demetrios Α.; Tsatsakis, Aristidis; Rezaee, Ramin

doi:10.3892/or.2017.5766

Cited by 398 publications

(256 citation statements)

References 89 publications

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“…The antitumor properties of flavonoids are discussed in the literature [21][22][23]. The data obtained by us are consistent with the results of other authors demonstrating that flavonoids activate apoptosis in tumor cells [24,25], while not having toxicity. Thus, it is known that the flavonoid Vogonin as part of the popular Chinese herbal remedy Huang-Qin (based on grass skullcap Baikal) induces apoptosis in a wide range of human tumor cells in vitro and inhibits tumor growth in vivo in various models of mouse tumors [3,[29][30][31][32][33][34].…”

Section: Discussionsupporting

confidence: 90%

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

Polukonova¹,

Navolokin²,

Bucharskaya³

et al. 2018

Russ Open Med J

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Objective -The discovery of the apoptosis-inducing effects of flavonoid vagonin allowed to make an assumption of existence of similar effect in others flavonoids. This study is devoted to the effects of Gratīola officinālis extract on cell culture of the human kidney cancer.Methods -Cell cultures of human kidney carcinoma -Caki-1 and SN12c were used in the study. The cells were stained with Hoechat 33258 dye. The number of living cells, cells in a state of apoptosis and mitosis were counted. The Cramer-Welch criterion (T) was used to compare the obtained data.Results -The activation of apoptosis was noted at all concentrations of the Gratiola officinalis L. extract during the first day of exposure. The apoptotic activity increased with increasing of extract concentration. After 48 hours, this activity was maintained only at a Gratiola officinalis L. extract concentration of 0.9 mg/ml. After 24 hours, the apoptotic activity of the extract was more expressed in the culture of CaKi-1. However, after 48 hours the extract induces more pronounced apoptosis in the culture of Sn12c cells. The cytotoxic activity of the extract was not differ after 24 hours in both cultures, after 48 hours it was more pronounced in the culture of CaKi-1. Conclusion -We revealed a pronounced antitumor and apoptotic activity of the Gratiola officinalis L. extract against the cultures of the kidney cancer Caki-1 and Sn12c. Apoptosis of tumor cells can be manifested in the form of pycnosis of the nucleus, the formation of apoptotic bodies and the emergence of cellular debris resulting from complete degradation of tumor cells. The apoptotic activity of the Gratiola officinalis extract depends on their concentration.

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Section: Discussionsupporting

confidence: 90%

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

Polukonova¹,

Navolokin²,

Bucharskaya³

et al. 2018

Russ Open Med J

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“…Recently, nutraceuticals have shown good cytotoxicity and selectivity in inhibition of various cancer cells in both in vitro assays and in vivo experiments . In view of this, NPs of 1–6 and corresponding bulk compounds were screened for their anticancer activity against hormone dependent human breast adenocarcinoma cell line MCF‐7 employing sulforhodamine B (SRB) assay method .…”

Section: Resultsmentioning

confidence: 99%

Anticancer, Antioxidant and Antiangiogenic Activities of Nanoparticles of Bioactive Dietary Nutraceuticals

et al. 2019

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Many nutraceuticals are highly potent, nontoxic, bioactive agents present in dietary vegetables, fruits and spices. They exhibit immune‐modulatory, antioxidant and anticancer activity. The major disadvantage of nutraceuticals is their low aqueous solubility and hence poor bioavailability hindering their therapeutic applications. In the present work, we herein report preparation of nanoparticles of bioactive nutraceuticals such as curcumin (1), quercetin (2), anthraquinone (3), xanthone (4), flavone (5) and vanillin (6) with a view to improve their aqueous‐phase solubility and evaluate their anticancer, antiangiogenic and antioxidant activities. The nanoparticles were prepared by controlled flow rate reprecipitation technique and characterized by spectral and microscopic techniques. Most of the nanoparticles displayed higher in‐vitro anticancer, antioxidant and in‐ovo antiangiogenic activities as compared to the corresponding bulk compounds. The biological activities of nutraceuticals are marked increased by particle size reduction to the nanoscale.

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“…Quercetin concentrations 10-80 µM are used under 24-h treatment conditions. Quercetin IC50 values of quercetin are 60-120 µM [36][37][38]. Especially, quercetin of 25-50 µM had anticancer effects in SAS cells, a typical oral cancer cell [39].…”

Section: Discussionmentioning

confidence: 99%

Quercetin Inhibits Cell Survival and Metastatic Ability via the EMT-Mediated Pathway in Oral Squamous Cell Carcinoma

et al. 2020

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This study aimed to investigate whether quercetin exerts anticancer effects on oral squamous cell carcinoma (OSCC) cell lines and to elucidate its mechanism of action. These anticancer effects in OSCC cells were assessed using an MTT assay, flow cytometry (to assess the cell cycle), wound-healing assay, invasion assay, Western blot analysis, gelatin zymography, and immunofluorescence. To investigate whether quercetin also inhibits transforming growth factor β1 (TGF-β1)-induced epithelial-mesenchymal transition (EMT) in human keratinocyte cells, HaCaT cells were treated with TGF-β1. Overall, our results strongly suggest that quercetin suppressed the viability of OSCC cells by inducing cell cycle arrest at the G2/M phase. However, quercetin did not affect cell viability of human keratinocytes such as HaCaT (immortal keratinocyte) and nHOK (primary normal human oral keratinocyte) cells. Additionally, quercetin suppresses cell migration through EMT and matrix metalloproteinase (MMP) in OSCC cells and decreases TGF-β1-induced EMT in HaCaT cells. In conclusion, this study is the first, to our knowledge, to demonstrate that quercetin can inhibit the survival and metastatic ability of OSCC cells via the EMT-mediated pathway, specifically Slug. Quercetin may thus provide a novel pharmacological approach for the treatment of OSCCs.Molecules 2020, 25, 757 2 of 15 Statistical AnalysesStatistical analyses were performed by a one-way analysis of variance (ANOVA) followed by a Bonferroni post-test, whereby three or more experimental groups were compared. p-values of < 0.05 were considered statistically significant. All data are the means ± standard error of the mean (SEM).

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Anticancer and apoptosis-inducing effects of quercetin in vitro and in vivo

Cited by 398 publications

References 89 publications

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

Anticancer, Antioxidant and Antiangiogenic Activities of Nanoparticles of Bioactive Dietary Nutraceuticals

Quercetin Inhibits Cell Survival and Metastatic Ability via the EMT-Mediated Pathway in Oral Squamous Cell Carcinoma

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