Anticancer activity of Moringa oleifera mediated silver nanoparticles on human cervical carcinoma cells by apoptosis induction

Vasanth, K.; Ilango, K.; Ramasamy, Mohankumar; Agrawal, Aruna; Dubey, Govind Prasad

doi:10.1016/j.colsurfb.2014.02.052

Cited by 228 publications

(113 citation statements)

References 40 publications

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“…In previous studies, the cytotoxic effects of AgNPs on cancer cells, along with the noncytotoxic effects of AuNPs, were similar to our findings. 26,27,[29][30][31] Suliman et al reported 32 AgNPs show less cytotoxicity on A549 cell compared with D-AgNPs at same concentration. Next, the synergistic effect between Dendropanax nanoparticles (silver and gold) and the ginsenoside CK was determined.…”

Section: In Vitro Cytotoxicity Of D-agnps and D-aunps In Lung Cancer mentioning

confidence: 99%

Rapid green synthesis of silver and gold nanoparticles using<em> Dendropanax morbifera</em> leaf extract and their anticancer activities

Kim¹,

Wang²,

Mathiyalagan³

et al. 2016

IJN

109

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Dendropanax morbifera Léveille is an oriental medicinal plant that is traditionally used in folk medicine and grows in a specific region of South Korea. We aimed to enhance the utilization of D. morbifera medicinal plants for synthesis of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs). D. morbifera leaf extract acted as both a reducing and a stabilizing agent that rapidly synthesized Dendropanax AgNPs (D-AgNPs) and Dendropanax AuNPs (D-AuNPs). The D-AgNPs and D-AuNPs were characterized by ultraviolet-visible spectroscopy, energy dispersive X-ray analysis, elemental mapping, field emission transmission electron microscopy, X-ray diffraction, and dynamic light scattering. The characterizations revealed that the D-AgNPs and D-AuNPs were in polygon and hexagon shapes with average sizes of 100–150 nm and 10–20 nm, respectively. The important outcomes were the synthesis of AgNPs and AuNPs within 1 hour and 3 minutes, respectively, avoiding the subsequent processing for removal of any toxic components or for stabilizing the nanoparticles. Additionally, D-AgNPs and D-AuNPs were examined for cytotoxicity in a human keratinocyte cell line and in A549 human lung cancer cell line. The results indicated that D-AgNPs exhibited less cytotoxicity in the human keratinocyte cell line at 100 µg/mL after 48 hours. On the other hand, D-AgNPs showed potent cytotoxicity in the lung cancer cells at the same concentration after 48 hours, whereas D-AuNPs did not exhibit cytotoxicity in both cell lines at the same concentration. However, both D-AgNPs and D-AuNPs at 50 µg/mL enhanced the cytotoxicity of ginsenoside compound K at 25 µM after 48 hours of treatment compared with CK alone. We believe that this rapid green synthesis of D-AgNPs and D-AuNPs is a valuable addition to the applications of D. morbifera medicinal plant. D-AuNPs can be used as carriers for drug delivery and in cancer therapy due to their lack of normal cell cytotoxicity.

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Section: In Vitro Cytotoxicity Of D-agnps and D-aunps In Lung Cancer mentioning

confidence: 99%

Rapid green synthesis of silver and gold nanoparticles using<em> Dendropanax morbifera</em> leaf extract and their anticancer activities

Kim¹,

Wang²,

Mathiyalagan³

et al. 2016

IJN

109

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“…The ROS generation was measured after staining the cells with oxidation sensitive probe 2-7-diacetyl dichlorofluorescein, Invitrogen, USA, (DCFH-DA) using flow cytometry as described previously with minor modifications (Krejsa and Schieven, 2000;Vasanth et al, 2014). THP-1 cells (2×10 5 cells/well) were cultured in 12-well plate treated with indicated concentrations in the presence or absence of T. chebula fruits extract (62.5, 125 and 250 µg mLG 1 ) and incubated at 37°C with 5% CO 2 for 24 h. The cells in 12-well plates were washed twice with Hank's Balanced Salt Solution (HBSS) and incubated in 1 mL working solution of 20 μM DCFH-DA at 37°C in the dark for 30 min.…”

Section: Quantification Of Intracellular Ros In H 2 O 2 Induced Thp-1mentioning

confidence: 99%

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Minakshi¹,

Vasanth²,

Tanupriya³

et al. 2014

International J. of Botany

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A B S T R A C T Diseases caused due to 'oxidative stress' initiated by Reactive Oxygen Species (ROS) is growing at an alarming rate. Several medicinal herbs explored till date were reported to possess numerous pharmacological properties used for combating different diseases. The present study was undertaken to investigate the inhibitory potential of Terminalia chebula by H 2 O 2 induced oxidative stress in THP-1 cell line and also the presence of the marker compounds in T. chebula was confirmed by High Performance Liquid Chromatography (HPLC). Intracellular quantification of ROS was done using a flow cytometer. The percentage of gallic acid and ellagic acid in the extract was performed by HPLC were found to be 4.84% w/w and 2.56% w/w, respectively. Pretreatment of cells with T. chebula extract significantly reduced the intracellular ROS production in a dose-dependent manner. The present study illustrates the effective role of T. chebula in reducing the ROS production which is a causative factor for several disorders. The results describe the possibility and potentiality of natural sources to inhibit the pathogenesis of different diseases caused due to oxidative stress.

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“…In Indian traditional medicine, the leaf extract of I. echioides was used as a remedy for fever (Kirtikar and Basu 1975) and also the plant from genus Andrographis is used to treat goiter, liver diseases (Nadkarni and Nadkarni 1976), fertility disorders, bacterial (Qadrie et al 2009), malarial and fungal diseases. These phytochemically synthesized SNPs have a potential effect such as good nanostructure (Huh and Kwon 2011) and antibacterial (Savithramma et al 2011), antioxidant (Swamy et al 2014) and anticancer activity (Vasanth et al 2014). …”

Section: Introductionmentioning

confidence: 99%

Biogenic synthesis and spectroscopic characterization of silver nanoparticles using leaf extract of Indoneesiella echioides: in vitro assessment on antioxidant, antimicrobial and cytotoxicity potential

et al. 2015

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Natural synthesis of metal nanoparticles is gaining more attention in recent years. This article demonstrates the phytochemical synthesis of silver nanoparticles (AgNPs) by using Indoneesiella echioides (L) leaf extract as a reducing and stabilizing agent. Biosynthesis of AgNPs was monitored by UV-visible spectroscopy which revealed intense surface plasmon resonance bands at 420 nm. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction were employed to identify various functional groups and crystalline nature of AgNPs. High-resolution transmission electron microscopy studies demonstrated that synthesized particles were spherical with average size of *29 nm. In vitro antioxidant effects were analyzed by 2,2 0 -Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), which exhibited 69 and 71 % of scavenging activity, respectively. The antimicrobial activity of green AgNPs displayed better zone of inhibition against selected human pathogens. The present study also investigated the toxicity effect of biogenic AgNPs against human lung adenocarcinoma cancer cells (A549) and normal human epithelial cells (HBL-100) in vitro, and the inhibitory concentrations (IC 50 ) were found to be 30 and 60 lg/mL, respectively. Herein, we propose a previously unexplored medicinal plant for the biological synthesis of AgNPs with potent biomedical applications.

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Anticancer activity of Moringa oleifera mediated silver nanoparticles on human cervical carcinoma cells by apoptosis induction

Cited by 228 publications

References 40 publications

Rapid green synthesis of silver and gold nanoparticles using<em> Dendropanax morbifera</em> leaf extract and their anticancer activities

Rapid green synthesis of silver and gold nanoparticles using<em> Dendropanax morbifera</em> leaf extract and their anticancer activities

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Biogenic synthesis and spectroscopic characterization of silver nanoparticles using leaf extract of Indoneesiella echioides: in vitro assessment on antioxidant, antimicrobial and cytotoxicity potential

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