Biogenic synthesis and spectroscopic characterization of silver nanoparticles using leaf extract of Indoneesiella echioides: in vitro assessment on antioxidant, antimicrobial and cytotoxicity potential

Abstract

Natural synthesis of metal nanoparticles is gaining more attention in recent years. This article demonstrates the phytochemical synthesis of silver nanoparticles (AgNPs) by using Indoneesiella echioides (L) leaf extract as a reducing and stabilizing agent. Biosynthesis of AgNPs was monitored by UV-visible spectroscopy which revealed intense surface plasmon resonance bands at 420 nm. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction were employed to identify various functional groups and crystalline nature of AgNPs. High-resolution transmission electron microscopy studies demonstrated that synthesized particles were spherical with average size of *29 nm. In vitro antioxidant effects were analyzed by 2,2 0 -Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), which exhibited 69 and 71 % of scavenging activity, respectively. The antimicrobial activity of green AgNPs displayed better zone of inhibition against selected human pathogens. The present study also investigated the toxicity effect of biogenic AgNPs against human lung adenocarcinoma cancer cells (A549) and normal human epithelial cells (HBL-100) in vitro, and the inhibitory concentrations (IC 50 ) were found to be 30 and 60 lg/mL, respectively. Herein, we propose a previously unexplored medicinal plant for the biological synthesis of AgNPs with potent biomedical applications.

Biosynthesis of Silver Nanoparticles Using Onion Endophytic Bacterium and Its Antifungal Activity against Rice Pathogen Magnaporthe oryzae

“…17 to 26.9 nm in the reaction mixture (Figure 3). The observations in the present study are 193 consistent with the results from the previously reports[15,[29][30][31]. Furthermore, EDS result showed 194 that the element peak of silver, silica and sulfur are 92.77, 5.53 and 1.70%, respectively, in the 195 reaction mixture(Figure 4).…”

Biogenic silver nanoparticles mediated by Broussonetia papyrifera: anticancer and antimicrobial activity against pathogenic organisms

“…The ability of the complexes to kill the cancer cells at 24 hrs incubation vary as silver nitrate, AgNPs, B. papyrifera. (Table 2) the results from this MTT assay indicate that the complexes silver nitrate, AgNPs are highly cytotoxic against MCF-7 cancer cells and lung cancer cell line (A549) similar results correlates with these findings [25]. The cytotoxicity of AgNPs shows that the increase with the concentration of AgNPs leads to the increase in the inhibition rate.…”

Cytotoxicity of Plant-Mediated Synthesis of Metallic Nanoparticles: A Systematic Review

“…Although the gold nanoparticles synthesised from the Dysosma pleiantha rhizome showed no cytotoxicity to human fibrosarcoma (HT1080) cells over the tested durations, the study highlighted their anti-metastatic property by cell migration inhibition via the Rac1-mediated actin polymerisation pathway [ 68 ]. Overall, the silver nanoparticles had a higher cytotoxicity potency than gold nanoparticles when the same plants ( Plumbago zeylanica , Commelina nudiflora , and Cassia auriculata ) were used for the synthesis, irrespective of the cancer cell type tested [ 5 , 14 , 71 ].…”

“…The plant-mediated synthesis of metallic nanoparticles has become increasingly recognised as a mean to produce cytotoxic agents to fight various cancers [ 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. However, the safety of these synthesised cytotoxic nanomaterials as a treatment remains a huge concern.…”