Antibody response in rats against non-toxic glucose sensor membranes tested in cell culture

Ziegler, Mario; Schlosser, Michael; Abel, Peter; Ziegler, B

doi:10.1016/0142-9612(94)90043-4

Cited by 18 publications

(18 citation statements)

References 16 publications

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“…Subsequent to previous studies where we were able to document IgG-antibodies against polymers (polyester, polyurethane, PTFE) in rats and in domestic pigs, 15,16,20 the question of humoral immune response to the coating or impregnation of vascular prostheses logically arose. This study focused upon the use of collagen due to the fact that bovine type I collagen, along with albumin and heat-denatured collagen, is widely used as a sealant for widelypored vascular prostheses.…”

Section: Discussionmentioning

confidence: 96%

Antibody response to collagen after functional implantation of different polyester vascular prostheses in pigs

Schlosser

Zippel²,

Hoene

et al. 2005

J Biomedical Materials Res

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Besides inflammation, specific immune responses are seen also after implantation of biomaterials. The aim was to investigate the humoral response to bovine collagen type I following implantation of various polyester (Dacron) prostheses into pigs. In 24 randomized pigs, the infrarenal aorta was replaced with a segment of collagen-impregnated, woven polyester prosthesis of low, medium, or high porosity. IgG antibodies were detected by immunoassay using native and denatured collagen type I as a target for blood samples taken on day 1 (implantation), 10, 17, 24, 62, and 116. As generally observed, antibodies to native and denatured collagen are of low titer and were significantly correlated with enhanced binding to the denatured form (p < 0.001). The highest overall antibody prevalence to native and denatured collagen was obtained on day 116 with 68% and on day 62 with 59%, respectively. Prostheses with high porosity induced an early immune response on day 10; those with low and medium porosity induced the highest antibody levels later after 2 months. Collagen antibodies neither correlated with serum IgG contents nor with antibodies to the prosthesis polyester matrix. Thus, humoral immune response against implant components may provide a further parameter in describing biocompatibility but also a potential marker that may facilitate monitoring of individual perigraft reaction.

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Section: Discussionmentioning

confidence: 96%

Antibody response to collagen after functional implantation of different polyester vascular prostheses in pigs

Schlosser

Zippel²,

Hoene

et al. 2005

J Biomedical Materials Res

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“…We recently detected specific polymer antibodies within the serum of recipient rats up to 49 days after the implantation of different polymeric membranes used for covering glucose sensors 7, 8. The intensity of the immune response, (e.g., antibody prevalence) is dependent not only on the various polymeric materials used but also on the individual recipient.…”

Section: Introductionmentioning

confidence: 99%

“…The intensity of the immune response, (e.g., antibody prevalence) is dependent not only on the various polymeric materials used but also on the individual recipient. Therefore, not only the cellular (immune) reaction but especially the humoral component, such as the detection of specific antibodies to biomaterial components, may serve as early markers for individual chronic inflammatory responses and, as such, perhaps warn of future implant failure 7–13…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity of polymeric implants: Long‐term antibody response against polyester (Dacron) following the implantation of vascular prostheses into LEW.1A rats

Schlosser

Wilhelm

Gunnarsson³

et al. 2002

J. Biomed. Mater. Res.

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Implanted biomaterials trigger acute and chronic inflammatory responses directly correlated to the central role of phagocytic cells at the host-implant interface. This study was designed to evaluate specific humoral immune responses following repeated intraperitoneal implantations of collagen-impregnated polyester (Dacron) prosthetic segments into LEWIS rats. Serum antibody detection was performed by enzyme immunoassay with the prosthetic segments as a target. Cutoff values for antibody positivity were greater than or equal to the 99th percentile for control rats. Polymer immunoglobiulin G (IgG) antibodies were significantly increased (p < 0.05) by repeated implantation and were subsequently followed until experimental day 293. Antibody formation was significantly enhanced through the application of complete Freund's adjuvant in combination with the first implantation. All rats within this group were antibody-positive on day 53, but only 6 of 10 animals that received the prosthesis without the adjuvant were. After preincubation of sera with bovine collagen type I (solid phase adsorbed or in solution), polymer antibody binding was discovered not to be diminished, indicating that the IgG antibodies detected were not directed against the prosthesis impregnation. Furthermore, a significant correlation was obtained between polymer antibody binding to collagen-impregnated and nonimpregnated prostheses (r(s) = 0.797, p < 0.001). There was no substantiated correlation between antibody binding to polyester and to an irrelevant polymer (Tecoflex EG 80). We conclude that specific polymer antibodies may indeed provide an additional parameter for biocompatibility testing as well as a possible serological marker of an inflammatory response to implants.

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“…The enzymes used in both sensors originate from non-mammalian proteins, and thus have the potential to cause an allergic response. [9][10][11] For this reason, we are interested in monitoring for enzyme loss from the sensors during fabrication.…”

Section: Introductionmentioning

confidence: 99%

Immobilization Techniques to Avoid Enzyme Loss from Oxidase-Based Biosensors: A One-Year Study

House

Anderson

Ward

2007

J Diabetes Sci Technol

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Background:Continuous amperometric sensors that measure glucose or lactate require a stable sensitivity, and glutaraldehyde crosslinking has been used widely to avoid enzyme loss. Nonetheless, little data is published on the effectiveness of enzyme immobilization with glutaraldehyde. Methods:A combination of electrochemical testing and spectrophotometric assays was used to study the relationship between enzyme shedding and the fabrication procedure. In addition, we studied the relationship between the glutaraldehyde concentration and sensor performance over a period of one year. Results:The enzyme immobilization process by glutaraldehyde crosslinking to glucose oxidase appears to require at least 24-hours at room temperature to reach completion. In addition, excess free glucose oxidase can be removed by soaking sensors in purified water for 20 minutes. Even with the addition of these steps, however, it appears that there is some free glucose oxidase entrapped within the enzyme layer which contributes to a decline in sensitivity over time. Although it reduces the ultimate sensitivity (probably via a change in the enzyme's natural conformation), glutaraldehyde concentration in the enzyme layer can be increased in order to minimize this instability. Conclusions:After exposure of oxidase enzymes to glutaraldehyde, effective crosslinking requires a rinse step and a 24-hour incubation step. In order to minimize the loss of sensor sensitivity over time, the glutaraldehyde concentration can be increased.

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Antibody response in rats against non-toxic glucose sensor membranes tested in cell culture

Cited by 18 publications

References 16 publications

Antibody response to collagen after functional implantation of different polyester vascular prostheses in pigs

Antibody response to collagen after functional implantation of different polyester vascular prostheses in pigs

Immunogenicity of polymeric implants: Long‐term antibody response against polyester (Dacron) following the implantation of vascular prostheses into LEW.1A rats

Immobilization Techniques to Avoid Enzyme Loss from Oxidase-Based Biosensors: A One-Year Study

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