Antibody response to collagen after functional implantation of different polyester vascular prostheses in pigs

Abstract

Besides inflammation, specific immune responses are seen also after implantation of biomaterials. The aim was to investigate the humoral response to bovine collagen type I following implantation of various polyester (Dacron) prostheses into pigs. In 24 randomized pigs, the infrarenal aorta was replaced with a segment of collagen-impregnated, woven polyester prosthesis of low, medium, or high porosity. IgG antibodies were detected by immunoassay using native and denatured collagen type I as a target for blood samples taken on day 1 (implantation), 10, 17, 24, 62, and 116. As generally observed, antibodies to native and denatured collagen are of low titer and were significantly correlated with enhanced binding to the denatured form (p < 0.001). The highest overall antibody prevalence to native and denatured collagen was obtained on day 116 with 68% and on day 62 with 59%, respectively. Prostheses with high porosity induced an early immune response on day 10; those with low and medium porosity induced the highest antibody levels later after 2 months. Collagen antibodies neither correlated with serum IgG contents nor with antibodies to the prosthesis polyester matrix. Thus, humoral immune response against implant components may provide a further parameter in describing biocompatibility but also a potential marker that may facilitate monitoring of individual perigraft reaction.

In vivo investigation of the inflammatory response against allylamine plasma polymer coated titanium implants in a rat model

“…The simultaneous implantation of the four implants into the same individual animals as performed in this study was similar to previous experiments on calcium-phosphate-coated titanium [14]. The advantage of this approach is that it eliminates the broad individual variability of host reactions which were observed, for example, in studies on the immunological reactions against vascular prostheses using inbred rats as well as pigs [16,17].…”

Avoiding Detrimental Human Immune Response Against Mammalian Extracellular Matrix Implants

“…[1][2][3][4][5][15][16][17][18] ECM molecules such as type I collagen, laminin, and fibronectin of xenogeneic origin were found to be immunogenic in mammals and induce antibody production. [19][20][21] Thus, it is possible that antibodies produced by the recipient against ECM molecules bind to implanted ECM and hinder by their physical presence the stem cell/ECM interaction. In addition, such ECM-bound antibodies may prevent stem cells interaction with specific ligands on the ECM.…”

Recent Developments in Cyclic Acetal Biomaterials for Tissue Engineering Applications

“…Polyesters and polyanhydrides, as well as other similarly structured polymers, degrade via hydrolysis and give rise to products with carboxylic acid terminal groups. Thus their degradation may create an acidic regenerative environment which can prolong the inflammatory response and accelerate the degradation of the material, leading to premature loss of mechanical and structural properties (54,55). Previous studies have also shown that accumulation and increased concentration of acidic degradation products can induce tissue toxicity (28,29,56).…”