Antibody response in cats to feline leukemia virus reverse transcriptase under natural conditions of exposure to the virus

Jacquemin, Paul; Saxinger, Carl; Gallo, Robert C.; Hardy, William; Essex, Max

doi:10.1016/0042-6822(78)90393-8

Cited by 25 publications

(15 citation statements)

References 22 publications

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“…It is known that infected cats are able to elicit antibodies against different components of FeLV (18)(19)(20)(21)(22). However, until now the detection of antibodies to FeLV had limited significance for several reasons: first, there is no evidence that reliable antibody detection can predict FeLV infection; second, it is not known which antibodies are suitable; and third, the existence of endogenous FeLV (enFeLV) is widespread in cat populations in that every cell in every single cat harbors multiple copies of enFeLV (23,24).…”

mentioning

confidence: 99%

Detection of Antibodies to the Feline Leukemia Virus (FeLV) Transmembrane Protein p15E: an Alternative Approach for Serological FeLV Detection Based on Antibodies to p15E

et al. 2014

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The aim of this report was to investigate whether the diagnosis of feline leukemia virus (FeLV) infection by serology might be feasible and useful. Among the various viral proteins, the FeLV env-gene product (SU) and the envelope transmembrane protein p15E were considered promising candidates for the serological diagnosis of FeLV infection. Thus, we evaluated p15E and three other FeLV antigens, namely, a recombinant env-gene product, whole FeLV, and a short peptide from the FeLV transmembrane protein, for their potential to detect FeLV infection. To evaluate possible exposure of cats to FeLV, we tested serum and plasma samples from experimentally and naturally infected and vaccinated cats for the presence of antibodies to these antigens by enzyme-linked immunosorbent assays (ELISAs). The serological results were compared with the p27 and proviral real-time PCR results. We found that p15E displayed a diagnostic sensitivity of 95.7% and a specificity of 100% in experimentally infected cats. In naturally infected cats, p15E showed a diagnostic sensitivity of 77.1% and a specificity of 85.6%. Vaccinated cats displayed minimal antibody levels to p15E, suggesting that anti-p15E antibodies indicate infection rather than vaccination. The other antigens turned out to be too unspecific. The lower specificity in cats exposed to FeLV under field conditions may be explained by the fact that some cats become infected and seroconvert in the absence of detectable viral nucleic acids in plasma. We conclude that p15E serology may become a valuable tool for diagnosing FeLV infection; in some cases, it may replace PCR.

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confidence: 99%

Detection of Antibodies to the Feline Leukemia Virus (FeLV) Transmembrane Protein p15E: an Alternative Approach for Serological FeLV Detection Based on Antibodies to p15E

et al. 2014

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“…The internal viral antigens are not known to have any significant biologic function, but antibodies to these antigens can be produced resulting in the formation of immune complexes and the development of glomerulonephritis or other detrimental immunopathologic conditions. The FeLV reverse transcriptase is also immunogenic although antibodies to this enzyme have no known beneficial or detrimental biologic effect [43,69].…”

Section: Felv and Felv-induced Antigensmentioning

confidence: 99%

Immunopathology induced by the feline leukemia virus

Hardy

1982

Springer Semin Immunopathol

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“…1A, lanes 1-19, all of the sera tested reacted with both the rEIAV p66 and p51 in the assays. In general, the reactivities of serum samples [6][7][8][9][10][11][12][13][14][15][16][17][18][19], obtained later during infection, were more intense than the reactivities seen with samples 1-5. None of the four control sera from uninfected horses reacted with the antigens (Fig.…”

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confidence: 99%

“…A standard panel of reference sera from 19 EIAV-infected horses (designated [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] were tested for reactivity with EIAV RT antigens in rEIAV RT immunoblot assays. The sera were collected as previously described.21 Serum samples 1-5 were acquired from experimentally infected ponies within the first year postinfection and reflect the immune status of animals early in infection, during active equine infectious anemia.…”

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confidence: 99%

High Prevalence of Serum Antibodies to Equine Infectious Anemia Virus Reverse Transcriptase

DeVico¹,

Issel²,

Grice³

et al. 1993

AIDS Research and Human Retroviruses

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The immunogenicity of the equine infectious anemia virus (EIAV) reverse transcriptase (RT) was examined by immunoblot assay with recombinant EIAV RT. All of the 19 sera from EIAV-infected horses tested contained antibodies that recognized EIAV RT and directly inhibited the polymerase activity of the enzyme. An examination of sera obtained sequentially from two experimentally infected animals revealed that anti-RT antibodies arise early in infection and increase in level. The appearance of the antibodies correlated with progression toward the asymptomatic period of infection.

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Antibody response in cats to feline leukemia virus reverse transcriptase under natural conditions of exposure to the virus

Cited by 25 publications

References 22 publications

Detection of Antibodies to the Feline Leukemia Virus (FeLV) Transmembrane Protein p15E: an Alternative Approach for Serological FeLV Detection Based on Antibodies to p15E

Detection of Antibodies to the Feline Leukemia Virus (FeLV) Transmembrane Protein p15E: an Alternative Approach for Serological FeLV Detection Based on Antibodies to p15E

Immunopathology induced by the feline leukemia virus

High Prevalence of Serum Antibodies to Equine Infectious Anemia Virus Reverse Transcriptase

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