Antibody purification with protein A attached supermacroporous poly(hydroxyethyl methacrylate) cryogel

Concanavalin A immobilized poly(ethylene glycol dimethacrylate) based affinity cryogel matrix and usability of invertase immobilization

“…While conventional chromatography techniques, which uses gel beads column chromatography have certain limitations such as a high pressure drop, low flow-rates and hence low productivities and difficulties in efficient scale up, cryogels provides a potential solution due to their low pressure drop and lack of diffusion resistances due to the macropores compared to traditional column chromatography [28].…”

Poly(hydroxyethyl methacrylate) based affinity cryogel for plasmid DNA purification

“…The large pores of cryogels will allow penetration of large pDNA molecules to the internal surface area at high flow rate with low pressure drops [27]. High accessibility to binding sites and negligible internal mass transfer limitations are also characteristics of cryogels, making them attractive for pDNA binding studies [28]. N-methacryloyl-(l)-histidine methyl ester [MAH] was used as a pseudospecific ligand and/or comonomer.…”

Molecular imprinting based composite cryogel membranes for purification of anti-hepatitis B surface antibody by fast protein liquid chromatography

“…Thus, the anti-HBs molecules have more time to diffuse to the pore walls of the CMs, to interact with embedded particles and to bind to the molecular cavities at lower flow-rates, hence a better adsorption capacity is observed. It should be noted that there is no pressure drop limitation because of supermacroporous characters of the CMs [33,34]. …”