Antibody Formation Against Vibrio Cholerae by Mesenteric Lymph Node Cells Maintained in Vitro*

Abstract

SUMMARY : Studies conducted by us have revealed that sensitized mesenteric lymph node (MLN) cells from donors, when put into tissue culture system, proceed to form antibody in vitro. The antibody produced by these cells was studied by the bacterial adherence phenomenon (BAP) and antibody hemolytic plaque (AHPT) techniques and it is estimated that about 40 % of these MLN cells are capable of producing antibody in vitro.The viability of MLN cells is essential for this antibody synthesis.The BAP and AHPT activity are present only in MLN cell cultures in immunized donors.The activity appears to be that of specific antibody which has been formed by these immunologically competent MLN cells during the in vitro incubation.

Mesenteric Panniculitis Associated With Vibrio cholerae Infection

“…An animal study demonstrated that immunization with Vibrio cholerae could yield mesenteric lymphadenopathy, and revealed that inoculated recipients formed Vibrio cholerae agglutinins in their mesenteric lymph nodes. 15 Two studies also found that immunization with cholera caused increased antibody formation by mesenteric lymph nodes, resulting in inflammation, 13 , 14 and that a systemic immune response preceded mesenteric lymph node response. 18 Immunization with Vibrio cholerae lipopolysaccharide (LPS) and enterotoxin in rabbits showed increased IgM synthesis that was specific to the spleen and mesenteric lymph nodes compared to controls.…”

Senior Overviews

“…This is indeed what Deutsch found. It has been interpreted, quite erroneously, as the first example of immune cell transfer [19,31,32]. One sentence in Deutsch's paper could give rise to this misinterpretation: 'In 7 cases we have injected into the peritoneal cavity of guinea-pigs spleens removed on the 3rd and 5th day after the immunizing injection' [7, p. 706].…”