Antibody-Dependent Lymphoid Cell-Mediated Cytotoxicity: No Requirement for Thymus-Derived Lymphocytes

Boxel, John A. van; Stobo, John D.; Paul, William E.; Green, Ira

doi:10.1126/science.175.4018.194

Cited by 185 publications

(61 citation statements)

References 28 publications

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“…Antibody-dependent direct lymphocyte-mediated cytotoxicity (ADDLC). Numerous experiments indicate that nonsensitized lymphocytes are able to induce cytotoxicity of cells coated with specific antibody (13)(14)(15)(16)(17)(18). This reaction is inhibited by aggregated gamma globulin (18,19).…”

Section: Distribution Of E and Eac Rosette-forming Cells Inmentioning

confidence: 99%

Fractionation of cells on a discontinuous Ficoll gradient. Study of subpopulations of human T cells using anti-T-cell antibodies from patients with systemic lupus erythematosus.

Gliński¹,

Gershwin²,

Steinberg³

1976

J. Clin. Invest.

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A B S T R A C T Patients with active systemic lupus erythematosus (SLE) had a decrease in a subpopulation of cells (fraction D) when peripheral blood lymphocytes were separated on a discontinuous Ficoll gradient. Preincubation of SLE cells at 370C for 30 min led to a marked decrease in this fraction, composed primarily of thymus-derived (T) cells. Supernates of such preincubations were found to cause a reduction in fraction D cells from normal humans. The active factor in the supernate was found to be an IgG antibody. Similarly, serum from patients with active SLE produced a reduction in fraction D cells from normal donors. This activity was also found in the IgG fraction, and could be absorbed with a pure T-cell population.Depletion of macrophages and complement did not reduce the SLE anti-T-cell antibody-mediated loss of cells from fraction D; however, heat-aggregated human gamma globulin led to impairment of the reaction. These findings suggest that antibody-dependent direct lymphocyte-mediated cytotoxicity may play a role in T-cell lymphopenia of SLE.It was further noted that the SLE anti-T-cell antibodies, in contrast to rabbit antihuman thymocyte serum, recognized fraction D cells but not fraction E cells from normals. Since both fractions are largely T cells, it appeared that the SLE serum was directed against cellmembrane antigenic determinants present on fraction D T cells, which were absent or reduced in quantity on

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Section: Distribution Of E and Eac Rosette-forming Cells Inmentioning

confidence: 99%

Fractionation of cells on a discontinuous Ficoll gradient. Study of subpopulations of human T cells using anti-T-cell antibodies from patients with systemic lupus erythematosus.

Gliński¹,

Gershwin²,

Steinberg³

1976

J. Clin. Invest.

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“…7 days after the last injection, the animals were bled and individual sera were tested (19) for their anti-0 titer. High titer sera were pooled before use, Anti-O Treatment of Spleen Cells.--Approximately 109 spleen cells were incubated at 37°C for 30 min in BSS which contained anti-8 serum of a concentration 10 times greater than that effective in killing 95% thymus cells.…”

Section: Antigen: Tolerogen and Immunogen--hgg Was Obtained Through mentioning

confidence: 99%

The Ability of Bacterial Lipopolysaccharide to Modulate the Induction of Unresponsiveness to a State of Immunity

Louis

Chiller

Weigle

1973

The Journal of Experimental Medicine

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There exists a body of evidence which details that the antibody response to a number of different antigens requires the cooperation between thymus-derived lymphocytes (T cells) and bone marrow-derived lymphocytes (B cells) in a reaction whose net effect is the production of specific antibody by B cells (1). Both of these lymphocyte classes can be made specifically unresponsive to a given antigen (2), although each specific population displays a distinct kinetic pattern in the induction and maintenance of unresponsiveness (3).Bacterial lipopolysaccharides (LPS) 1 are mitogenic for B lymphocytes (4, 5), a phenomenon which may be related to their ability to circumvent the requirement of T cells for antibody formation to antigens which normally require cellular cooperation. The latter has been demonstrated both in vivo or in vitro using such T celldependent antigens as sheep erythrocytes (6-8), haptens (9, 10), and serum proteins (11). LPS can also prevent immunological unresponsiveness. This effect was originally described by Claman (12), who observed that mice which were given LPS after a normally tolerogenic dose of bovine gamma globulin (BGG) did not become tolerant. Golub and Weigle (13) subsequently defined the temporal relationship between the injection of tolerogen and LPS and furthermore demonstrated that the effect of LPS on the induction of tolerance was independent of its activity on the phagocytic index of the reticuloendothelial system.

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“…Several studies have showvn that the effector cell in ADCC is a non-T cell and does not require prior sensitization (10)(11)(12)(13). Attempts to identify the effector population within the non-T cell populations have yielded seemingly conflicting results.…”

mentioning

confidence: 99%

“…Attempts to identify the effector population within the non-T cell populations have yielded seemingly conflicting results. van Boxel et al (10), using anti-O antisera, suggested the possibility that the effector cell is a bone marrow-derived (B) cell or a subpopulationi of B cells. Consistent with this theory, both Perlmann et al (9) and Schirrmacher et al (11) reported that the effector cells from mouse spleens are absorbed onto anti-immunoglobulin columns.…”

mentioning

confidence: 99%

Human antibody-dependent cellular cytotoxicity. Isolation and identification of a subpopulation of peripheral blood lymphocytes which kill antibody-coated autologous target cells.

Brier¹,

Chess²,

Schlossman³

1975

J. Clin. Invest.

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Antibody-Dependent Lymphoid Cell-Mediated Cytotoxicity: No Requirement for Thymus-Derived Lymphocytes

Cited by 185 publications

References 28 publications

Fractionation of cells on a discontinuous Ficoll gradient. Study of subpopulations of human T cells using anti-T-cell antibodies from patients with systemic lupus erythematosus.

Fractionation of cells on a discontinuous Ficoll gradient. Study of subpopulations of human T cells using anti-T-cell antibodies from patients with systemic lupus erythematosus.

The Ability of Bacterial Lipopolysaccharide to Modulate the Induction of Unresponsiveness to a State of Immunity

Human antibody-dependent cellular cytotoxicity. Isolation and identification of a subpopulation of peripheral blood lymphocytes which kill antibody-coated autologous target cells.

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