Fractionation of cells on a discontinuous Ficoll gradient. Study of subpopulations of human T cells using anti-T-cell antibodies from patients with systemic lupus erythematosus.

Gliński, W; Gershwin, M. Eric; Steinberg, Alfred D.

doi:10.1172/jci108316

Cited by 81 publications

(39 citation statements)

References 32 publications

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“…Several studies have emphasized the importance of anti-T cell antibodies in altering T cell number and function in patients with active SLE (7,8,(28)(29)(30)(31)(32)(33)(34). In this regard, recent studies demonstrated that anti-T cell antibodies found in the sera of active SLE patients were selectively reactive with the T5+/T8+ suppressor T cell population (8) and patients with active SLE had decreased numbers of T5+/T8+ subsets (19).…”

Section: Discussionmentioning

confidence: 99%

Autoantibody to an Immunoregulatory Inducer Population in Patients with Juvenile Rheumatoid Arthritis

Morimoto¹,

Reinherz²,

Borel³

et al. 1981

J. Clin. Invest.

109

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A B S T R A C T The human inducer (T4+) and reciprocal cytotoxic/suppressor (T5+/T8+) subsets have been defined by monoclonal antibodies. In the present study, we examined the relationship of naturally occurringanti-T cell autoantibodies found in patients with active juvenile rheumatoid arthritis (JRA) to these subsets. In one approach, normal T cells were treated with anti-T4 or anti-T8 to eliminate the corresponding subset of cells and then analyzed for reactivity with JRA sera. It was found that JRA sera were reactive with only 15% of an enriched cytotoxic/suppressor population, whereas they reacted with 37% of an enriched inducer population. In reciprocal studies, JRA+ T cells were eliminated with JRA sera and complement and the residual T cells (JRA-) reacted with monoclonal antibodies and indirect immunofluorescence on a fluorescence-activated cell sorter. As expected, the JRA sera and complement treatment of unfractionated T cells markedly diminished the T4+ subset, whereas there was a concomitant increase in T cells reactive with anti-T5 and anti-T8. A similar diminution in T4+ T cells was found in the circulating peripheral T cell compartment of patients with active JRA who possessed the JRA antibody.Functional studies demonstrated that removal of the JRA+ population of T cells diminished phytohemagglutinin and soluble antigen proliferative responses,

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Section: Discussionmentioning

confidence: 99%

Autoantibody to an Immunoregulatory Inducer Population in Patients with Juvenile Rheumatoid Arthritis

Morimoto¹,

Reinherz²,

Borel³

et al. 1981

J. Clin. Invest.

109

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“…An additional defect in Tnony cells, perhaps induced by anti-T cell antibodies (25)(26)(27)(28)(29)(30), may further impair T cell control mechanisms, thereby allowing uncontrolled B cell hyperactivity. The failure of B cells from such active SLE patients to stimulate in the autologous MLR may be a related inability of activated B cells to stimulate autologous T cells.…”

Section: Discussionmentioning

confidence: 99%

Studies of immune functions of patients with systemic lupus erythematosus

Sakane

Steinberg

Arnett

et al. 1979

Arthritis & Rheumatism

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In normal individuals T cells are stimulated to proliferate by autologous non-T cells; this is called the autologous mixed lymphocyte reaction (MLR). Previous studies demonstrated that such an autologous MLR was markedly impaired in patients with active systemic lupus erythematosus (SLE). To determine whether the defect resided in the responding cell or the stimulating cell, mixing experiments were performed using cells from identical twins. We identified two sets of identical twins discordant for SLE activity and correspondingly discordant in their degree of responsiveness in the autologous MLR. Reciprocal mixing experiments were performed in which T cells from one twin of each pair were mixed with non-T cells from the other twin of that pair. These studies indicated that patients with active SLE

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“…Demonstrable abnormalities in CMI are more prominent during periods of active disease, with partial or complete resolution during remission (6,7). Considerable indirect evidence suggests that autoantibodies directed toward lymphocyte surface determinants may be of fundamental importance in this regard (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). The present consensus is that there is no intrinsic defect in the lymphocytes per se, although this issue is controversial and by no means resolved.…”

Section: Role Of Anti-lymphocyte Antibodies In Abnormal Immune Systemmentioning

confidence: 99%

Significance of anti-lymphocyte antibodies in systemic lupus erythematosus

Winfield

Lobo

Singer

1978

Arthritis & Rheumatism

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Sera from patients with SLE frequently contain IgM and IgG antibodies with multiple specificities for lymphocyte surface determinants, including autologous antigens. The IgM antibodies are of relatively low binding avidity and exhibit broad reactivity with B and T lymphocytes from most individuals. IgG antibodies are reactive selectively with PBL from different individuals and appear to be more specific for B cell and a minor proportion of T cells. The molecular nature of the surface determinants involved and their relationship with known antigens and receptors remain largely undefined.Interest in anti-lymphocyte antibodies in SLE relates in part to data suggesting a causal role in the abnormal immune system function in this disorder. In this regard, possible mechanisms that are supported by indirect data include: a) antibody-mediated lymphocyte depletion in vivo, perhaps involving functional subsets specifically; b) antibody blockade of surface receptors operant in cell-

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Fractionation of cells on a discontinuous Ficoll gradient. Study of subpopulations of human T cells using anti-T-cell antibodies from patients with systemic lupus erythematosus.

Cited by 81 publications

References 32 publications

Autoantibody to an Immunoregulatory Inducer Population in Patients with Juvenile Rheumatoid Arthritis

Autoantibody to an Immunoregulatory Inducer Population in Patients with Juvenile Rheumatoid Arthritis

Studies of immune functions of patients with systemic lupus erythematosus

Significance of anti-lymphocyte antibodies in systemic lupus erythematosus

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