Antibody degradation in wound exudates from blowfly infections on sheep

Sandeman, R.M.; Chandler, Rosemary; Turner, Nilgun; Seaton, D.S.

doi:10.1016/0020-7519(94)00160-p

Cited by 17 publications

(8 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pruett (3) showed that Hypodermin A cleaves bovine IgG. In the same manner, Lucilia cuprina (Calliphoridae) larvae contain digestive tryptic and chymotryptic proteases involved in larval nutrition (4) and avoidment of cellular (5,6) and humoral immune responses, with cleavage of IgG (7–9). Moreover, excretion of ammonia by L. cuprina larvae suppresses immunity in sheep (10).…”

Section: Introductionmentioning

confidence: 99%

Oestrus ovis (Diptera: Oestridae): effects of larval excretory/secretory products on nitric oxide production by murine RAW 264·7 macrophages

Tabouret

Vouldoukis

Duranton

et al. 2001

Parasite Immunology

View full text Add to dashboard Cite

Larvae of Oestrus ovis (Insecta: Diptera: Oestridae) are common parasites of nasal and sinus cavities of sheep and goats. Previous studies revealed that crude extracts of larvae modify NO synthesis by ovine monocyte derived macrophages. The aim of this study was to investigate the larval excretory/secretory products effects on nitric oxide production by murine tumour macrophages RAW 264.7. Stimulation of RAW macrophages by excretory/secretory products of the three instars larvae (25 microg/ml) significantly increased nitrite concentrations in culture supernatants compared to negative and positive Escherichia coli lipopolysaccharide control. This effect was time and dose dependent. Nitrite production in culture supernatants was due to induction of isoform NOS-2 because both NG monomethyl L-arginine (100 microM) and dexamethasone (20 microM) inhibited, by 60 and 50%, respectively, nitrite accumulation in culture supernatants. First steps of purification, by ion exchange chromatography, indicated that one protein of 29 kDa was able to induce NO synthesis by macrophages. Further studies are needed for a better characterization of these molecule and to investigate their immunogenicity for a vaccine approach.

show abstract

Section: Introductionmentioning

confidence: 99%

Oestrus ovis (Diptera: Oestridae): effects of larval excretory/secretory products on nitric oxide production by murine RAW 264·7 macrophages

Tabouret

Vouldoukis

Duranton

et al. 2001

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…Previous work on D. hominis adaptive immune response on cattle showed the same tendency, with 90% of the animals presenting antibodies against D. hominis prior to experimental infestation (CORONADO-FONSECA, 1989). SANDEMANN et al (1995), as well as BARON & NELSON (1985), seeking for antibodies titres to Lucilla cuprina and Melophagus ovinus, respectively, in experimentally infested sheep observed gradual augments of serum antibodies, which decreased on few weeks after exposition. These authors concluded that adaptive immune response against L. cuprina and M. ovinus should occurs only when larval infestation was active.…”

Section: Resultsmentioning

confidence: 80%

Humoral immune response in cattle experimentally infested with larvae of Dermatobia Hominis

2000

View full text Add to dashboard Cite

Six bovines were infested with 60 first instar larvae of Dermatobia hominis. The animals were bleed weekly, and their antibodies levels to D. hominis L1, L2 and L3 instars measured during the time, following the infestation course. The antisera were submitted to a titration against optimal dilutions of antigen coated wells of microplates, previously sensitized with L1, L2 and L3 preparations, respectively. The ELISA assay was used to test single dilutions of antisera, which results were comparatively analyzed with a control of not infested animals. Antibodies against L1 were detected between the first and 21st day post-infestation (DPI) and, from the 42nd DPI on. Anti-L2 antibodies, could be detected on the 21st DPI and from the 35th DPI until approximately the 49th DPI, when it was observed a decreasing of antibodies titration equivalent to the control group. No antibodies were detected against the L3 instar-antigens. Antibodies levels against L1 showed absorbance higher than 1.500 O.D. at 492nm in the ELISA assay, when compared to the 0.096 O.D. observed to the negative animals. High anti-L2 antibodies were also detected on the 21st DPI, where two animals showed O.D. of 0.450 and 0.900 at 492nm, with a cut-off estimated on 0.110 O.D. It was also demonstrated a rising of anti-L2 antibodies in the same four animals, which presented antibodies response against L1 instar. The obtained results, with an estimated prevalence of 50%, were comparatively evaluated, taking the double diffusion immunoassay precipitation test as a standard, and showed a concordance of 98%. The association between infestation and presence of specific antibodies was also discussed.

show abstract

“…A number of reports identified the production of serum antibodies to various larval antigens post-vaccination and subsequent antilarval growth effects in vitro (54-61); however, these results generally did not translate into significant protection following implants with first-stage larvae in vivo. The reasons for this apparent inability to translate in vitro effects to the in vivo situation were suggested to include insufficient antibody titres present in the skin, the degradation of IgG at the wound site and the time required to reach peak antibody titres in the sera (40,62,63). In contrast to the reports where vaccination failed to illicit a protective response in vivo was the study by Bowles et al (64), in which vaccination with four partially purified antigens induced an 86% (Trial 1) and a 67% (Trial 2) reduction in the incidence of strike compared to unvaccinated controls.…”

Section: Vaccine Responsesmentioning

confidence: 99%

The immunobiology of myiasis infections – whatever happened to vaccination?

Sandeman

Bowles

Colwell³

2014

Parasite Immunology

View full text Add to dashboard Cite

The current state of myiasis vaccine technologies are reviewed mainly in the primary research genera of Lucilia and Hypoderma. The importance of myiasis flies as primary causes of morbidity and mortality in agricultural species and man has not diminished despite the existence of good control strategies. However, the development of vaccines against myiasis infections has been relatively quiescent for more than 10 years despite the rapid development of genomic and proteomic analysis and of skills in data interpretation. The value of vaccine research in an era of chemical primacy is analysed. In fact, recent findings of drug resistance and the impact of animal welfare concerns should mean a renewed interest in alternative controls. The reasons that this has not been true to date are explored and new possibilities discussed.

show abstract

Antibody degradation in wound exudates from blowfly infections on sheep

Cited by 17 publications

References 9 publications

Oestrus ovis (Diptera: Oestridae): effects of larval excretory/secretory products on nitric oxide production by murine RAW 264·7 macrophages

Oestrus ovis (Diptera: Oestridae): effects of larval excretory/secretory products on nitric oxide production by murine RAW 264·7 macrophages

Humoral immune response in cattle experimentally infested with larvae of Dermatobia Hominis

The immunobiology of myiasis infections – whatever happened to vaccination?

Contact Info

Product

Resources

About