Antibiotic susceptibility of bacterial strains isolated from patients with community-acquired urinary tract infections

Daza, Roberto; Gutiérrez, J; Piédrola, G

doi:10.1016/s0924-8579(01)00389-2

Cited by 124 publications

(84 citation statements)

References 15 publications

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“…The analyzed results of antibiotic susceptibility test showed that E. coli was highly resistant to gentamicin (73.69%), ampicillin (73.69%), SXT (69.74%), and cephalothin (88.16%). This is similar to previous studies in USA (25) and Iran (17). On the other hand, very low resistancewas detected to antibiotics such as ciprofloxacin (26.32%), imipenem (15.79%), nitrofurantoin (9.22%), and ceftizoxime (15.79%).…”

Section: Discussionsupporting

confidence: 93%

Antibiotic Susceptibility of Bacterial Strains Isolated From Urinary Tract Infections in Karaj, Iran

Khoshbakht

Salimi

Aski

et al. 2012

Jundishapur J Microbiol

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The article will help Clinicians in predicting and planning correct strategies for UTI therapy.Backgrounds: Urinary Tract Infection (UTI) is one of the most common bacterial diseases worldwidethat can be presented asymptomatic or symptomatic, characterized by a wide range of symptoms from mild irritative voiding to bacteremia, sepsis, or even death. In latter decades, widespread utilization of antibiotics has resulted in increased incidence of antibiotic resistance among urinary tract pathogens all over the world. Objectives: The aim of this study was to determine patterns of antibiotic resistance bacteria isolated from urinary tract infections in the city of Karaj, Iran. Materials and Methods: This descriptive study was conducted in the city of Karaj from November 2009 to August 2010. Bacterial isolates were identified by standard biochemical tests. Antibacterial susceptibility test was performed by Kirby-Bauer method against some common antibiotics for Gram negative and Gram positive bacteria and the data were analyzed by SPSS software, version 12.0.1. Results: In this study, 230 (8.06%)out of 2852 patients showed positive urine cultures of which there were 204 (88.69%) females and 26 (11.3%) males. 180 (83.17%) cases of isolated bacteria were Gram negative bacilli while 50 (21.73%) cases were Gram positive cocci. E. coli isolates, as the most frequent Gram negative bacteria, showed high resistance to cephalothin (88.16%) and high susceptibility to nitrofurantoin (90.78%). Also Staphylococcus saprophyticus isolates, as the most frequent Gram positive bacteria, exhibited high resistance to ampicillin, tetracycline, and erythromycin (92.31%) and high susceptibility to nitrofurantoin and vancomycin (92.3%). Conclusions: considering the results of this study, E.coli isolates were the predominant pathogens in UTI. Our data demonstrated that antibiotics with the highest activity included ciprofloxacin and nitrofurantoin and those with the least activity included ampicillin and sulfamethoxazole-trimethoprim. Our findings reinforce the need for ongoing investigations to show the trends of antibiotic resistance which can help antibiotic prescription in the clinics.

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Section: Discussionsupporting

confidence: 93%

Antibiotic Susceptibility of Bacterial Strains Isolated From Urinary Tract Infections in Karaj, Iran

Khoshbakht

Salimi

Aski

et al. 2012

Jundishapur J Microbiol

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“…According to a Turkish study [27], E. coli isolates were highly resistant to ampicillin (47.8% to 64.6%) and higher resistance rates to ampicillin have been reported in other countries including Senegal (77%), Spain (65%), Taiwan (80%), and India (88%) [28][29][30][31]. In our study, the ampicillin resistance rate was 88%.…”

Section: Discussionsupporting

confidence: 55%

Antimicrobial susceptibility patterns of community-acquired uropathogens in Tehran, Iran

Kashef

Djavid

Shahbazi

2010

J Infect Dev Ctries

119

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Background: Antibiotic resistance of urinary tract pathogens has increased worldwide. Knowledge of the antibiotic resistance patterns of uropathogens in specific geographical locations is an important factor for choosing an appropriate empirical antimicrobial treatment. The aim of this study was to provide information regarding local resistance patterns of urinary pathogens to the commonly used antibiotics in Tehran, Iran. Methodology: Urine samples collected and submitted to two pathobiology laboratories in Tehran were identified by conventional methods over a period of three years (December 2006 to May 2009). Antimicrobial resistance testing was performed by the standard disk diffusion technique in accordance with the recommendations of the Clinical and Laboratory Standards Institute. Results: Of the total 13,333 mid-stream urine samples collected from suspected cases of urinary tract infection, 840 (6.3%) were positive for pathogenic bacteria. Escherichia coli (E. coli) was the most common isolate (68.8%) followed by Proteus spp. (12.4%), and Klebsiella spp. (9.6%). E. coli isolates were mostly susceptible to nitrofurantoin (71.3%), followed by ciprofloxacin (68.1%); however, only 38.2% of E. coli isolates were susceptible to trimethoprim-sulfamethoxazole. Conclusion: Nitrofurantoin may be considered as a first-line empiric antibacterial agent for urinary tract infections in outpatients in Tehran, Iran.

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“…High resistance pattern for AMP reported with a percentage of 62%, [16] found the resistance to AMP was 56% which is near to our results, [17] reported 80%. This was explained by the overuse of antibiotics especially AMP in Erbil Hospitals, and also to the missuses of these antibiotic as they are prescribed without sensitivity test [18]. In the present work, 33% of the isolates were resistant to NA, this result was in agreement with [19] who stated that 29 % of P. mirabilis isolates were resistant for NA, also [17] found that 20 % of P. mirabilis isolates were resistant to NA.…”

Section: B Antibiotic Resistance Profilesupporting

confidence: 89%

Curing Analysis of Drug Resistance Plasmid in Proteus Mirabilis

Omer¹,

Abdulrahman²,

Saeed³

2017

Cihan Univ.-Erbil Sci. J.

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Forty five isolates of Proteus mirabilis were identified among 600 samples taken from patients with urinary tract infection from different hospitals in Erbil City. Isolates were identified from urine sample by using cultural, morphological and biochemical characteristics and confirmed confirmed by VITEK 2 systems. Antibiotic sensitivity testing was done for all isolates by using fifteen antibiotic types which included Amikacin (AK), Ampicillin (AMP), Aztreonam (AT), Chloramphenicol (C), Cephalothin (CEP), Ciprofloxacin (CIP), Ceftriaxone (CTR), Cefotaxime (CTX) Fosfomycin (FOX), Gentamicin (GEN), Imipenem (IMP), Nalidixic acid (NA), Norfloxacin (NOR), Piperacilin (PI) and Tobramycin (TOB). IMP was the most effective antibiotic against isolated of P. mirabilis. The resistance rate of the isolates toward these antibiotics were 8.8% for CIP, 11% for AT, 11% for FOX, 15.5% for NOR, 17.7% for TOB, 20% for AK, 22% for PI, 26% for CTR, 28.8% for CTX, 33% for NA, 35.5% for GEN, 44% for C, and 62% for AMP respectively. To control the antibiotic resistance of the tested P. mirabilis isolates, curing of plasmid DNA was conducted using tetracycline and elevated temperature at 46 o C, two most resistance isolates were chosen for this purpose P32, and P40, then treated with different concentrations of tetracycline (0.5, 1, 1.5, 2, 2.5, 3, 3.5, and 4) µg/ml separately. The MIC for tetracycline was 2.5µg/ml and SMIC 2µg/ml was used as a curing agent. The genes encoded resistance to ampicillin, chloramphenicol, cephalothin, ceftriaxone, cefotaxime, gentamicin and tobramycin were cured from P32 and the percentage of curing was (46.6%), while amikacin, chloramphenicol, ciprofloxacin and gentamicin resistance genes were cured from P40 isolate and the curing percent was (26.6%).The results confirmed by conducting gel electrophoresis and revealed that tetracycline DOI: 10.24086/cuesj.si.2017.n2a5 47 removed plasmid of P32, while it had no effect on plasmid of P40. On the other hand, elevated temperature used also as curing agent and the results revealed that resistance to amikacin, ampicillin, chloramphenicol, cephalothin, ceftriaxone, gentamicin and tobramycin genes were cured from P32 and the curing percent was (46.6%), while genes encoding amikacin, ampicillin, chloramphenicol, ciprofloxacin, ceftriaxone, cefotaxime, fosfomycin, gentamicin, nalidixic acid, norfloxacin and tobramycin were cured from P40 isolate with the percentages of (73.3%) after incubating the isolates at 46 o C. It is clear that elevated temperature is the most efficient method than tetracycline. The results confirmed by conducting gel electrophoresis and showed that both tested isolates lost their plasmids after incubation at 46 o C.

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Antibiotic susceptibility of bacterial strains isolated from patients with community-acquired urinary tract infections

Cited by 124 publications

References 15 publications

Antibiotic Susceptibility of Bacterial Strains Isolated From Urinary Tract Infections in Karaj, Iran

Antibiotic Susceptibility of Bacterial Strains Isolated From Urinary Tract Infections in Karaj, Iran

Antimicrobial susceptibility patterns of community-acquired uropathogens in Tehran, Iran

Curing Analysis of Drug Resistance Plasmid in Proteus Mirabilis

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