Antibiogram of Staphylococcal Strains Isolated from Milk and Milk‐Products

Abstract

Summary A study of the resistance patterns of 248 staphylococcal isolates from milk and milk products to eight antimicrobial agents using the disc method showed that 80 (32.3 %) were resistant to sulphafurazole, 75 (30.2 %) to penicillin G, 63 (25.4 %) to ampicillin, 23 (9.3 %) to cloxacillin, 19 (7.7 %) to tetracycline, 17 (6.9%) to streptomycin, 14 (5.6%) to erythromycin and 12 (4.8%) to chloramphenicol. One hundred and sixteen (46.8 %) of the 248 staphylococcal isolates were sensitive to all the agents tested. A significant percentage (P < 0.05) of the isolates from raw milk were resistant to erythromycin, sulphafurazole, cloxacillin, penicillin G and streptomycin compared to isolates from fermented milk. The minimum inhibitory concentrations (MIC) as determined by the tube method for isolates resistant by the disc method, were ≥ 16 μg for ampicillin in 5 (11.9 %) isolates; ≥ 40 μg for cloxacillin, 9 (36.0%) and ≥ 12 international units for penicillin G amongst 12 (22.2%) isolates. MIC values of ≥ 40 μg were recorded for 9 (90.0%), 9 (69.2%), 8 (73.7%) and 7 (70.0%) isolates to chloramphenicol, streptomycin, erythromycin and tetracycline respectively. The relatively high level of resistance to antimicrobial agents is a reflection of misuse or abuse of these agents in the environment.

Pre-processed bovine milk quality in Trinidad: Prevalence and characteristics of bacterial pathogens and occurrence of antimicrobial residues in milk from collection centres

“…Comparatively higher prevalence of resistance to antimicrobial agents amongst S. aureus strains have been reported by others (Gentilini, Demamiel, Llorente, & Godaly, 2000;Shitandi & Sternesjo, 2004;Umoh et al, 1990b).…”

“…The antimicrobial resistance of S. aureus and E. coli recovered from raw milk and milk products have been documented by others (Adesiyun, Webb, Romain, & Kaminjolo, 1997;Makovec & Ruegg, 2003;Pitkala, Haveri, Pyorala, & Myllys, 2004;Umoh, Adesiyun, & Gomwalk, 1990b) and it has also been reported that misuse or abuse of antimicrobial agents could result in resistant strains of bacteria in veterinary medicine (Washington, 1979).…”

The prevalence of bacterial resistance in clinical, food, water and some environmental samples in Southwest Nigeria

“…The number of antibiotics involved in the multiple drug resistance as obtained in this study, falls within the range obtained by earlier workers (Grewal and Tiwari, 1990;Singh et al, 1995;Khan and Malik, 2001). The relatively high level of resistance to antimicrobial agents could be a reflection of misuse or abuse of these agents in the environment (Umoh et al, 1990;Abbar and Kaddar, 1991;Silva and Hoffer, 1993;Malik and Ahmad, 1994). Antibiotic prescriptions in hospitals are given without clear evidence of infection or adequate medical indication.…”

Antimicrobial resistance of bacterial strains isolated from orange juice products

“…The results of the antibiotic sensitivity test were interpreted and are presented as the resistance of bacterial isolates to the antibiotics (Table 1), and the antibiotic resistance pattern among the bacterial isolates (Table 2). In several earlier works on similar studies carried out on foodstuffs including orange juices, researches have been focused mainly on the strains of E. coli and S. aureus (Umoh et al, 1990;Grewal and Tiwari, 1990;Abbar and Kaddar, 1991;Singh et al, 1995;Desai and Kamat, 1998;Leegar et al, 1999;Silva et al, 2000;Rahman Khan and Malik, 2001). However, in this work, a broad overview of the study was conducted and this is why the resistance pattern and the mechanisms in selected strains of all the bacteria isolated from the orange juices were studied.…”

“…In several studies that have been conducted on the resistance of food-borne pathogens, emphasis had been on E. coli and S. aureus (Umoh et al, 1990;Grewal and Tiwari, 1990;Abbar and Kaddar, 1991;Singh et al, 1995;Desai and Kamat, 1998;Leegar et al, 1999;Silva et al, 2000;Rahman Khan and Malik, 2001). However, in the present study, an attempt was made to evaluate the resistance of selected strains of all the bacterial strains that were isolated from the orange fruit products.…”