Pre-processed bovine milk quality in Trinidad: Prevalence and characteristics of bacterial pathogens and occurrence of antimicrobial residues in milk from collection centres

Adesiyun, Abiodun A.; Stoute, Simone; David, Brien C.

doi:10.1016/j.foodcont.2005.10.012

Cited by 21 publications

(16 citation statements)

References 37 publications

(51 reference statements)

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“…E. coli O157 was not isolated from any of the samples in the current study, a finding similar to the reports of (Mankee et al 2005) [47] on 'doubles' in Trinidad. The organism has however been isolated from bulk milk in Trinidad where three (0.8%) out of three-hundred and eighty-six (386) samples were positive for E. coli O157 (Adesiyun et al 2007) [48].…”

Section: Discussionmentioning

confidence: 99%

“…E. coli O157 has also been isolated from the water supply to rural communities (2.0%) (Welch et al 2000) [13], local delicacies such as oysters (2%) (Rampersad et al 1999) [14] and 'black pudding' (13.6%) (Adesiyun and Balbirsingh 1996 [9]) and from pre-processed cows' milk (0.8%) (Adesiyun et al 2007) [12]. The first case of neonatal septicaemia and meningitis due to L. monocytogenes was reported in Trinidad in 1998, (Ashiru and Bratt 1998) [15] when symptoms appeared three days after a blood transfusion.…”

Section: Introductionmentioning

confidence: 99%

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Microbial Load and the Prevalence of Escherichia coli, Salmonella spp. and Listeria spp. in Ready-to-Eat Products in Trinidad

Hosein¹,

Muñoz²,

Sawh³

et al. 2008

TOFSJ

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This study was designed to determine the prevalence and microbial load of Listeria spp., Escherichia coli O157 and Salmonella spp. in ready-to-eat products in supermarkets across Trinidad. The microbial load was assessed using the total aerobic plate count (TAPC) per g/ml of foods and prevalence of Escherichia coli O157 and Salmonella spp. determined using conventional methods. For Listeria monocytogenes, immunomagnetic separation (IMS), TECRA (enzymelinked immunosorbent assay, ELISA) and conventional methods were used. The log 10 mean ± sd TAPC per g or ml was highest for vegetables (11.0±11.6), and lowest for seafood (5.2±5.7) (p < 0.05). The prevalence of L. monocytogenes was 1.7 %. Sixteen (4.5%) of 153 samples yielded E. coli but all samples were negative for Salmonella spp. and E. coli O157. The high microbial load and isolation of L. monocytogenes and E. coli from popular RTE foods could pose a health risk to consumers in the country.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbial Load and the Prevalence of Escherichia coli, Salmonella spp. and Listeria spp. in Ready-to-Eat Products in Trinidad

Hosein¹,

Muñoz²,

Sawh³

et al. 2008

TOFSJ

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“…No leite coletivo amostrado, a presença de resíduos de antimicrobianos foi detectada em 86% dos tanques de expansão e latões. Estudos conduzidos em Trinidad & Tobago (Adesiyun et al 2005) em amostras de leite de vacas em produção coletivo convencional, detectaram a presença de antimicrobianos em 4,2% da amostragem. A alta ocorrência de resíduos de antimicrobianos no leite de vacas na região amostrada na Colômbia é um achado preocupante, visto que os resíduos de fármacos podem causar reações orgânicas indesejáveis nos humanos, assim como determinam prejuízos na indústria para a produção de derivados lácteos (Santos & Fonseca 2007).…”

Section: Detecção De Resíduos De Antimicrobianosunclassified

Qualidade do leite de vacas criadas no sistema silvipastoril no Vale do Cauca, Colômbia

et al. 2014

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O sistema silvipastoril caracteriza-se por aumentar a produção de leite, com maior número de vacas por hectare devido ao maior aporte de proteína na dieta. Neste sistema as vacas são alimentadas, além do pasto, de pequenas árvores e arbustos. O objetivo do presente estudo foi determinar os principais indicadores de qualidade do leite e agentes causais de mastite em vacas criadas em sistema silvipastoril. Foram avaliadas a composição (teor de gordura, proteína total, lactose, extrato seco, extrato seco desengordurado e nitrogênio uréico), contagem de células somáticas (CCS), contagem bacteriana total (CBT), ocorrência de mastite clínica e subclínica, isolamento microbiológico, perfil de sensibilidade bacteriana "in vitro" e detecção de resíduos antimicrobianos no leite produzido por 100 vacas, bem como do tanque de expansão e latões em propriedades do Vale do Cauca, Colômbia. Os teores médios dos principais constituintes do leite foram 3,24% de gordura, 3,27% de proteína total, 4,40% de lactose, 10,62% de extrato seco, 8,57% de extrato seco desengordurado e 15,82mg/dL de nitrogênio uréico, enquanto do tanque de expansão e latões foi 3,51% de gordura, 3,20% de proteína total, 4,34% de lactose, 11,72% de extrato seco, 8,47% de extrato seco desengordurado e 14,57mg/dL de nitrogênio uréico. A celularidade média dos quartos mamários e do tanque de expansão foi 141.252,75 CS/mL e 363.078,05 CS/mL respectivamente. A CBT média dos quartos mamários e do tanque de expansão foi 4.466,84 UFC/mL e 24.547,01 UFC/mL. Os principais micro-organismos isolados dos quartos mamários foram Corynebacterium bovis, Staphylococcus aureus, Staphylococcus hyicus, Staphylococcus spp., Streptococcus dysgalatiae, enquanto do tanque de expansão foram identificados Streptococcus spp., Enterobacter cloacae, Hafnia alveii, Streptococcus hemolítico e Streptococcus spp., com maior frequência. A presença de resíduos de antimicrobianos em leite de vacas e do tanque ou latão foi detectada em 30% e 86% das amostras, respectivamente. O sistema silvipastoril mostrou ser uma boa alternativa para produção de leite em vacas. No entanto, são necessários cuidados no tratamento mamário para evitar resíduos no leite e a análise de todos os parâmetros de qualidade para garantir um produto diferenciado.

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“…In recent years, antibiotics resistance in bacteria of animal origin and its impact on human health have drawn much attention worldwide [1]. Bovine mastitis is the most common cause for the use of antibiotics agents in lactating dairy cattle [2] and the detection of antibiotics residues in milk poses health hazards to consumers and of high economic importance because such milk unfit for processing and subsequent consumption [3]. Moreover, the antibiotic therapy has many complications, such as hypersensitivity, direct toxicity, antibioticinduced immunosuppresion and super-infections.…”

Section: Introductionmentioning

confidence: 99%

Development of New Strategy for Non-Antibiotic Therapy: Bovine Lactoferrin Has a Potent Antimicrobial and Immunomodulator Effects

Hafez¹,

Ismael²,

Mahmoud³

et al. 2013

AID

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The antibiotic therapy has many problems, such as antibiotics resistance, hypersensitivity, direct toxicity, antibioticinduced immunosuppresion and super-infections. This is highlighting the need for a new strategy for non-antibiotic therapy through the use of novel immunomodulators as naturally released ones (Lactoferrin). The present study investigates the potential of bovine lactoferrin (bLf), isolated from bovine milk whey, to prevent Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), Streptococcus agalactiae (S. agalactiae) and Pseudomonas aeruginosa (P. aerogenosa) growth and then evaluates its immunomodulator effect. First, bLf isolation was attempted from bovine milk whey using a cation exchange chromatography by SP-Sepharose. Second, the antimicrobial activity assays were trailed to study the antimicrobial activity of bLf. Finally, the immune effect of bLf was studied by lymphocyte transformation test. It was found that bLf was separated around molecular weight of 80 kDa and showed significant inhibitory effect against E. coli followed by P. aeruginosa, S. agalactiae and S. aureus. bLf increased lymphocyte transformations mean values in a dose dependant manner. The highest transformations mean value was determined at 50 µg/mL. In conclusion, these results suggest that bLf is a potent natural antimicrobials and immunomodulator agent.

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Pre-processed bovine milk quality in Trinidad: Prevalence and characteristics of bacterial pathogens and occurrence of antimicrobial residues in milk from collection centres

Cited by 21 publications

References 37 publications

Microbial Load and the Prevalence of Escherichia coli, Salmonella spp. and Listeria spp. in Ready-to-Eat Products in Trinidad

Microbial Load and the Prevalence of Escherichia coli, Salmonella spp. and Listeria spp. in Ready-to-Eat Products in Trinidad

Qualidade do leite de vacas criadas no sistema silvipastoril no Vale do Cauca, Colômbia

Development of New Strategy for Non-Antibiotic Therapy: Bovine Lactoferrin Has a Potent Antimicrobial and Immunomodulator Effects

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