Antibacterial Screening of Bacterial Isolates Associated with Mangrove Soil from the Ngurah Rai Mangrove Forest Bali

Abstract

In this study we reported cultivation of bacteria associated with mangrove soil from the Ngurah Rai Mangrove Forest, Bali. Mangrove soil samples were serially diluted using sterile artificial seawater, spread onto Starch Casein M agar and incubated at 28oC for 28 days. Cultivation of mangrove soil samples yielded 165 bacterial colonies with 68 isolates were selected and purified based on different morphology. Of these 68 isolates, 22 isolates displayed antibacterial activities ranging from weak to strong inhibition against at least one of four bacterial indicators namely Staphyloccocus aureus, Streptococus mutans, Escherichia coli and Klebsiella pneumoniae using perpendicular streak method. Overall, 19 out of 22 bacteria isolates displayed weak antibacterial potential and two isolates exhibited moderate antibacterial activity. The isolate SA4 was the only bacterium with strong antibacterial potential with measured clear distance ≥ 10 mm against the four bacterial isolates. Sequence analysis based on 16S rRNA gene fragment assigned the isolate SA4 as Bacillus subtilis strain BIL/BS-168. Overall, this study confirmed the untapped potential of antibacterial activities from bacteria associated with mangrove soil.

Antibacterial Activity Screening Of Bacterial Isolates Associated With Seaweed Eucheuma cottonii From Coastal Area In Buleleng, Bali

“…Furthermore, four bacterial indicator strains namely Staphylococcus aureus ATCC 25923, Streptococcus mutans FNCC 0405 Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603 were streaked horizontally from the tested isolates with distance of 3 cm (Figure 2). Subsequently, agar plates were incubated for 24 hours at 37ºC and the length of the inhibition zone (in mm) formed on each test bacteria was measured [28,29]. The zone of inhibition was calculated based on the distance of bacterial growth by bacterial isolates to each of the tested bacteria.…”

Pseudomonas sp., Moraxella sp., Vibrio sp. Dari Sedimen Mangrove Sebagai Antibakteri Terhadap Escherichia coli, Staphylococcus aureus dan Salmonella thypi

“…tindakan penanganannya (Romano et al 2018). Resistensi disebabkan oleh meningkatnya imun sehingga mengakibatkan terjadinya mutasi, transfer gen dan inaktivasi enzim (Indraningrat et al, 2021). Menurut World Health Organization (2019), E. coli menyebabkan kematian pada 370.000 anak akibat infeksi saluran pencernaan.…”