Antibacterial properties and mechanism of selenium nanoparticles synthesized by Providencia sp. DCX

Zhang, Henglin; Zheng, Li; Dai, Chunxiao; Wang, Ping; Fan, Shuling; Yu, Bin; Qu, Yuanyuan

doi:10.1016/j.envres.2020.110630

Cited by 82 publications

(56 citation statements)

References 47 publications

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“…The size of Se-NPs determines their potential biological activity, and it seems that Se-NPs with small particle size (particle size smaller than 100 nm) and good stability have higher biological activity because the increase of Se-NP diameter can reduce their antioxidant activity, Se retention ability, and upregulation of GSH S-transferase activity . Furthermore, the diameter of yeast-derived Se-NPs was smaller than the Se-NPs synthesized with Pseudomonas putida KT2440 (266 nm) reported by Avendaño et al and Se-NPs synthesized with Bacillus cereus (150–200 nm) reported by Dhanjal and Cameotra, and others. − Where the size ranges 85–709 nm − and the range between 100 and 300 nm is found significantly abundant, , it indicated that yeast can better control the size of the Se-NPs.…”

Section: Resultsmentioning

confidence: 88%

“…As shown in Figure c, the zeta potential of Se-NP solution was −34.00 mV. It is noteworthy that the strong surface charge (positive and negative, above 30 mV) is beneficial to inhibit the agglomeration of NPs . Considering the relationship between the stability of NPs and their zeta potential, Se-NPs can be stabilized in solution for a long time …”

Section: Resultsmentioning

confidence: 95%

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Synthesis, Characterization, Immune Regulation, and Antioxidative Assessment of Yeast-Derived Selenium Nanoparticles in Cyclophosphamide-Induced Rats

Ren

Liang

et al. 2021

ACS Omega

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This article introduces an environmentally friendly and more economical method for preparing red selenium nanoparticles (Se-NPs) with high stability, good biocompatibility, and narrow size using yeast as a bio-reducing agent with high antioxidant, immune regulation, and low toxicity than inorganic and organic Se. The yeast-derived Se-NPs were characterized by scanning electron microscopy, transmission electron microscopy, energy dispersive spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The results revealed spherical-shaped particles of Se-NPs with an average diameter of 71.14 ± 18.17 nm, an amorphous structure, and surface enhancement with an organic shell layer, that provide precise geometry and stability in the formation of bio-inert gray or black Se-NPs instead of red Se-NPs. Furthermore, the addition of 0.3−0.8 mg/kg Se-NPs in the feed significantly improved the health of mice. As Se-NPs stimulated the oxidative state of mice, it significantly increased the level of GSH-Px, SOD, and AOC, and decreased the level of MDA. The yeast-derived Se-NPs alleviated the immunosuppression induced by cyclophosphamide, whereas protected the liver, spleen, and kidney of mice, stimulated the humoral immune potential of the mice, and significantly increased the levels of I g M, IgA, and I g G. These results indicated that the yeast-derived Se-NPs, as a trace element feed additive, increased the defense of the animal against oxidative stress and infectious diseases and therefore Se-NPs can be used as a potential antibiotic substitute for animal husbandry.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 95%

Synthesis, Characterization, Immune Regulation, and Antioxidative Assessment of Yeast-Derived Selenium Nanoparticles in Cyclophosphamide-Induced Rats

Ren

Liang

et al. 2021

ACS Omega

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“…Thus, higher concentrations increase the area of contact, resulting in more antibacterial effects. These effects have been reported to observe the damaging of cell membranes of the microorganism [ 34 , 66 ]. SeNPs have been reported to generate a high level of ROS providing the anti-cancer properties of the nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

“…SeNPs have been reported to generate a high level of ROS providing the anti-cancer properties of the nanoparticles. Additionally, few studies have reported ROS also to possess antibacterial properties [ 66 ]. This investigation provides a proof of concept for the use of SeNPs as an effective antimicrobial coating on implants.…”

Section: Discussionmentioning

confidence: 99%

An In Vitro Evaluation of Selenium Nanoparticles on Osteoblastic Differentiation and Antimicrobial Properties against Porphyromonas gingivalis

Hou

Tamura

et al. 2022

Nanomaterials

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Despite numerous treatment methods, there is no gold standard for the treatment of peri-implantitis—an infectious peri-implant disease. Here, we examined selenium nanoparticles (SeNPs) at a wide range of concentrations to investigate their cytotoxicity, regulation of osteoblastic differentiation, and assessed the antibacterial effect against Porphyromonas gingivalis. SeNPs (mean size: 70 nm; shape: near-spherical; concentration: 0–2048 ppm) were tested against the MC3T3-E1 osteoblast precursor cell line and P. gingivalis red complex pathogen. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) analysis was used to evaluate the bone morphogenetic protein 2 (BMP-2) signaling pathway. SeNPs at concentrations of 2–16 ppm showed no obvious cytotoxicity and promoted good mineralization and calcification. SeNPs at concentrations 64 ppm and below influenced gene expression promoting osteoblastic differentiation, whereas at high concentrations inhibited the expression of Runt-related transcription factor 2 (Runx2). The growth of P. gingivalis was significantly inhibited at SeNP concentrations of more than 4 ppm. SeNPs at low concentrations promoted osteoblastic differentiation while strongly inhibiting peri-implantitis pathogen growth. This study represents one of the few in vitro assessments of SeNPs against a red complex pathogen and the regulatory effect on osteoblastic differentiation. The findings demonstrate SeNPs could potentially be used for future application on implant coating.

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“…SeNPs caused the leakage of proteins and polysaccharides after reacting with bio-SeNPs by altering membrane permeability and disrupting bacterial cell walls. Also, changes in the intensity of reactive oxygen species (ROS) by SeNPs could induce antibacterial effects [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Antibacterial and Antibiofilm Activity of Selenium Nanoparticles against Vibrio cholerae as a Potent Therapeutics

Bagheri-Josheghani

Bakhshi

2022

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Vibrio cholerae is a major cause of severe diarrhea, which is ecologically flexible, and remains as a major cause of death, especially in developing countries. Consecutive emergence of antibiotic-resistant strains is considered to be as one of the major concerns of the World Health Organization (WHO). Nanoparticles as a new nonantibiotic therapeutic strategy have been widely used in recent years to treat bacterial infections. The present study aimed to investigate the antibacterial and antibiofilm effect of selenium nanoparticles (SeNPs) in vitro against V. cholerae O1 ATCC 14035 strain. SeNPs were prepared and characterized using ultraviolet-visible (UV-Vis) spectroscopy, DLS (dynamic light scattering), zeta potential measurement, and Fourier transform infrared (FTIR) analysis. The concentration of SeNPs was calculated by ICP (inductively coupled plasma) method. Also, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was employed to assess the cytotoxic effect of SeNPs on Caco-2 cells. Antibacterial and antibiofilm activity of SeNPs was determined by broth microdilution and crystal violet assays, respectively. The average particle size of SeNPs was 71.1 nm with zeta potential −32.2 mV. The SEM images supported the uniform spherical morphology of the prepared nanoparticles. The antibiofilm effect of SeNPs was evident at concentrations of 50–200 μg/mL. This study results provided evidence that SeNPs are safe as an antibacterial and antibiofilm agent against V. cholerae O1 ATCC 14035 strain.

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Antibacterial properties and mechanism of selenium nanoparticles synthesized by Providencia sp. DCX

Cited by 82 publications

References 47 publications

Synthesis, Characterization, Immune Regulation, and Antioxidative Assessment of Yeast-Derived Selenium Nanoparticles in Cyclophosphamide-Induced Rats

Synthesis, Characterization, Immune Regulation, and Antioxidative Assessment of Yeast-Derived Selenium Nanoparticles in Cyclophosphamide-Induced Rats

An In Vitro Evaluation of Selenium Nanoparticles on Osteoblastic Differentiation and Antimicrobial Properties against Porphyromonas gingivalis

Investigation of the Antibacterial and Antibiofilm Activity of Selenium Nanoparticles against Vibrio cholerae as a Potent Therapeutics

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