Antibacterial interactions of pulegone and 1,8‐cineole with monolaurin ornisin against Staphylococcus aureus

Abstract

The aim of this study was to investigate the antibacterial interactions of pulegone and 1,8‐cineole with monolaurin ornisin against Staphylococcus aureus . The individual and combined antibacterial activities of the compounds were evaluated using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), fractional inhibitory concentration index (FICi), and time‐kill methods. Furthermore, the mechanism of the antibacterial action of the compounds was tested by measuring the release of cell constituents. The MIC values of pulegone, 1,8‐cineole, nisin, and monolaurin were 5.85 µl/ml, 23.43 µl/ml, 6.25 µg/ml, and 0.031 mg/ml, respectively. A synergistic antibacterial activity (FICi = 0.5) was found between 1,8‐cineole and nisin. The time‐kill assay showed that the populations of S. aureus exposed to 1,8‐cineole, nisin, and their combination were decreased by 5.9, 5.3, and 7.1 log CFU (colony‐forming units)/mL, respectively. The combination of 1,8‐cineole and nisin also induced the highest release of cell constituents. It was concluded that the combination of 1,8‐cineole and nisin could be considered as a novel and promising combination which may reduce the required dose of each antibacterial compound.

HS‐SPME coupled with GC–MS for elucidating differences between the volatile components in wild and cultivated Atractylodes chinensis

“…1,8‐Cineole exhibits a potential antimalarial effect with an impact on the parasite erythrocytic cycle and severe disease 41 . 1,8‐Cineole with nisin is a promising novel combination, which could reduce the required dose of each antibacterial compound 42 …”

“…41 1,8-Cineole with nisin is a promising novel combination, which could reduce the required dose of each antibacterial compound. 42 In conclusion, these substances involved in the monoterpene biosynthesis pathway and down-regulated in AC play important roles in the treatment of diseases. Improved breeding/cultivation management is critical to strengthen the corresponding metabolic pathways for enhancing the quality of artificially cultivated Atractylodes chinensis.…”

Essential oils isolated from popular medicinal plants and spices as alternative antimicrobial and antibiofilm compounds against the pig pathogen Actinobacilluspleuropneumoniae

“…For example, studies with Laurus nobilis conducted in different locations around the world have shown that 1.8-cineole is always the main compound in laurel essential oil, but its proportion can be as small as 30% or as high as 68%, the latter being close to the values observed in this work. This variation in oil composition is likely related to differences in antimicrobial activities observed against various Gram-negative and Gram-positive pathogens (ÖZCAN & CHALCHAT, 2005;SHOKOOHINIA et al, 2014;STEFANOVA et al, 2020), as 1, 8 -cineole alone is well characterized by its antimicrobial properties (FARHANGHI et al, 2022).…”

Construction of tea tree oil/salicylic acid/palygorskite hybrids for advanced antibacterial and anti-inflammatory performance

“…TSP-1 was further examined, together with TTO-Pal and SA-Pal, to investigate the drug release behaviors of SA and TTO oxygenated ingredients (p-cymene, 1,8-cineole and terpinene 4-ol), which are considered to have a significant influence on its functionality as a topical delivery system (1,8-cineole and terpinene 4-ol are well-known antibacterial agents while SA, terpined-4-ol and 1,8-cineole have been reported to possess anti-inflammatory activities). [52][53][54][55] As shown in Fig. 7 shows that under mimicking skin physiological conditions (pH 5.4, 32 1C), TSP-1 demonstrated a rapid release approaching nearly 100% within the initial 5 h, especially for terpined-4-ol and SA with maximum releases of 92.67% and 93.96%, respectively.…”