Antibacterial effects of Saponaria officinalis extracts against avian pathogenic Escherichia coli (APEC)

Abstract

In current study using Saponaria officinalis (Related to Caryophyllaceae family) hydroalcoholic extracts for in vitro growth inhibition of an avian isolated fatal Escherichia coli (isolated from coli CRDcomplex carcasses) were reported. The disc diffusion method was used for evaluation of the antibacterial effects of Saponaria extracts in comparison to some routine antibiotics. Minimum inhibitory concentration (MIC) of Saponaria extracts against avian pathogenic E. coli, APEC, using tube dilution technique was studied. In titration technique for MIC, the concentration of the extracts was estimated in v/v in Dimethyle sulfoxide (DMSO) as inert solvent. For minimum bacterial concentration (MBC) studies 0.1 ml of MIC titer beside, a tube before and after, MIC titer were used for cultivation on Muller Hinton agar. Based on the results, the zones of inhibition for 10, 15 and 20 µl of Saponaria disks were 16, 18 and 19 mm, respectively, and the zones of inhibition diameter for antibiotic disks of Fosbac (Fos), Oxytetracycline (Oxy) and Danofloxacin (Dfx) were 15, 0 and 14 mm, respectively. In MIC technique, it is oriented that the concentration of 1/80 inhibits APEC growth but for MBC, the bacterial growth on Muller Hinton agar was not stopped. The present study has revealed that saponin extract from S. officinalis has useful antibacterial effects.

Saponin-Based, Biological-Active Surfactants from Plants

“…For example, saponins from Yucca exhibit antimicrobial activity against Gram-positive cells but do not act on Gram negative bacteria. However, S. officinalis extracts showed antibacterial action against Gram negative, avian pathogenic Escherichia coli (APEC) strains [68,69]. In general, the antibacterial activity of saponins is often weak, whereas significant antifungal activity has been observed.…”

Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli

“…For this reason, the aim of this study was to evaluate the effect of cork functionalized with M. oleifera seed extract (MoSe), which as far as we know has not yet been elucidated, since studies with this material have been mainly focused on its use as a biocatalytic functional matrix with silver nanoparticles that efficiently reduced the growth of E. coli [23]. However, to confirm the antimicrobial effect of MoSe, it is necessary to apply faster methods than conventional ones to demonstrate the membrane cell disturbance caused by the bioactive compounds present in the seed extract [24,25]. Studies have reported that among these could be phytochemical compounds with antimicrobial properties that can reduce high cellular concentrations of pathogenic bacteria such as E. coli, Salmonella sp.…”

The Investigation of the Antibacterial Activity of Eryngium caucasicum trautv, Saponaria officinalis, Froriepia subpinnata, Malva sylvestris, and Urtica dioica

“…In a study by Veda et al, ethyl acetate extract of S. officinalis delivered the inhibition zone diameters of 12.67 ± 0.33, 14, 12.3 ± 0.33, 16, and 13.67 ± 0.33 mm against L. monocytogenes, Staphylococcus aureus, Streptococcus pneumoniae, E. coli, and Pseudomonas aeruginosa, while the inhibition zone diameters of the methanolic extract of this plant against these bacteria were obtained as 13.33 ± 0.33, 14.23 ± 0.33, 16, 19.67 ± 0.33, and 17.67 ± 0.33 mm, respectively (30). In another study, Nabinejad, who investigated the antimicrobial activity of S. officinalis extract against E. coli, reported that the inhibition zone diameters in the dilutions of 10, 15, and 20 microliters were equal to 16, 18, and 19 mm, respectively (31).…”