Antibacterial effect of silver nanoparticles on Staphylococcus aureus

Li, Wen-Ru; Xie, Xiaobao; Shi, Qingshan; Duan, Shunshan; Ouyang, Yu; Yi-ben, Chen

doi:10.1007/s10534-010-9381-6

Cited by 402 publications

(250 citation statements)

References 30 publications

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“…PC well-containing chloramphenicol shows a zone of 19 mm Figure 7. These results agreed with the previous work carried out by Khalil et al and Li et al [3,5]. The difference in the diameter of the zone of inhibition observed in these bacterial species is due to the difference in their cell wall composition [1].…”

Section: Resultssupporting

confidence: 93%

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Biosynthesis, characterization and antibacterial activity of silver nanoparticles from Aspergillus awamori

Vishwanatha¹,

Keshavamurthy²,

Mahanthappa³

et al. 2018

J App Biol Biotech

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Microbial nanoparticles (NPs) have become the subject of immense research interest in the recent past due to their wide range of applications as effective antimicrobial agents, drug delivery systems, gene delivery, diagnostic agents in imaging diseases, and consumer products among others. The present study emphasizes on the synthesis of metallic silver NPs (SNPs) from cell-free supernatant of Aspergillus awamori strain KGSR12. The phase purity, composition, size, and shape of the as-synthesized NPs were characterized using various analytic spectroscopic techniques including X-ray diffraction, scanning electron microscope (SEM), ultraviolet-visible, and Fourier transform-infrared spectroscopy. Based on the SEM analysis, the particles are uniformly distributed, and size is estimated to be 40-50 nm. Antibacterial activity of NPs against significant human pathogens was conferred with well diffusion assay, and it reveals the strains of Pseudomonas aeruginosa, Klebsiella pneumoniae, and Bacillus cereus are susceptible to synthesized SNPs that confirm the antibacterial activity of SNPs. Thus, the study concludes with the biogenic and eco-friendly route for synthesizing SNPs with antibacterial activity against clinically important pathogens and attributes growing interest on fungi as an emerging source for the synthesis of NPs.

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Section: Resultssupporting

confidence: 93%

“…High energy consumption and use of toxic chemicals limit the use of physical and chemical synthesis methods, respectively. Hence, an alternative approach based on biogenic principles can be employed for safe, cost-effective, and eco-friendly process to synthesize NPs using biological entities and its metabolites [5].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis, characterization and antibacterial activity of silver nanoparticles from Aspergillus awamori

Vishwanatha¹,

Keshavamurthy²,

Mahanthappa³

et al. 2018

J App Biol Biotech

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“…Antibacterial mechanisms of antibiotics are wellknown. 57 Multiple mechanisms have been suggested to explain the antibacterial activity of AgNPs, such as release of silver ions from AgNPs, 16 generation of reactive oxygen species, disruption of cellular morphology, inactivation of vital enzymes, DNA condensation, 11 and loss of DNA replication. 58 Emerging resistance among bacteria renders the available antibiotics inefficient.…”

Section: Shigella Sonnie Mtcc 2957mentioning

confidence: 99%

Synthesis, optimization, and characterization of silver nanoparticles from Acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics

Singh¹,

Wagh²,

Wadhwani³

et al. 2013

IJN

125

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Background The development of nontoxic methods of synthesizing nanoparticles is a major step in nanotechnology to allow their application in nanomedicine. The present study aims to biosynthesize silver nanoparticles (AgNPs) using a cell-free extract of Acinetobacter spp. and evaluate their antibacterial activity. Methods Eighteen strains of Acinetobacter were screened for AgNP synthesis. AgNPs were characterized using various techniques. Reaction parameters were optimized, and their effect on the morphology of AgNPs was studied. The synergistic potential of AgNPs on 14 antibiotics against seven pathogens was determined by disc-diffusion, broth-microdilution, and minimum bactericidal concentration assays. The efficacy of AgNPs was evaluated as per the minimum inhibitory concentration (MIC) breakpoints of the Clinical and Laboratory Standards Institute (CLSI) guidelines. Results Only A. calcoaceticus LRVP54 produced AgNPs within 24 hours. Monodisperse spherical nanoparticles of 8–12 nm were obtained with 0.7 mM silver nitrate at 70°C. During optimization, a blue-shift in ultraviolet-visible spectra was seen. X-ray diffraction data and lattice fringes ( d =0.23 nm) observed under high-resolution transmission electron microscope confirmed the crystallinity of AgNPs. These AgNPs were found to be more effective against Gram-negative compared with Gram-positive microorganisms. Overall, AgNPs showed the highest synergy with vancomycin in the disc-diffusion assay. For Enterobacter aerogenes , a 3.8-fold increase in inhibition zone area was observed after the addition of AgNPs with vancomycin. Reduction in MIC and minimum bactericidal concentration was observed on exposure of AgNPs with antibiotics. Interestingly, multidrug-resistant A. baumannii was highly sensitized in the presence of AgNPs and became susceptible to antibiotics except cephalosporins. Similarly, the vancomycin-resistant strain of Streptococcus mutans was also found to be susceptible to antibiotic treatment when AgNPs were added. These biogenic AgNPs showed significant synergistic activity on the β-lactam class of antibiotics. Conclusion This is the first report of synthesis of AgNPs using A. calcoaceticus LRVP54 and their significant synergistic activity with antibiotics resulting in increased susceptibility of multidrug-resistant bacteria evaluated as per MIC breakpoints of the CLSI standard.

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“…The antibacterial property of silver (Ag) NPs has frequently been used for numerous applications such as wound dressings, other medical devices, textiles or plastics as they fi ght both Gram positive and Gram negative bacteria, as well as fungi and viruses (35). The actual mechanism of their bactericide property has not been fully clarifi ed yet.…”

Section: Genotoxic Potential Of Silver Npsmentioning

confidence: 99%

Genotoxicity of Metal Nanoparticles: Focus on In Vivo Studies

Klien

Godnič‐Cvar

2012

Archives of Industrial Hygiene and Toxicology

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With increasing production and application of a variety of nanomaterials (NMs), research on their cytotoxic and genotoxic potential grows, as the exposure to these nano-sized materials may potentially result in adverse health effects. In large part, indications for potential DNA damaging effects of nanoparticles (NPs) originate from inconsistent in vitro studies. To clarify these effects, the implementation of in vivo studies has been emphasised. This paper summarises study results of genotoxic effects of NPs, which are available in the recent literature. They provide indications that some NP types cause both DNA strand breaks and chromosomal damages in experimental animals. Their genotoxic effects, however, do not depend only on particle size, surface modifi cation (particle coating), and exposure route, but also on exposure duration. Currently available animal studies may suggest differing mechanisms (depending on the duration of exposure) by which living organisms react to NP contact. Nevertheless, due to considerable inconsistencies in the recent literature and the lack of standardised test methods -a reliable hazard assessment of NMs is still limited. Therefore, international organisations (e.g. NIOSH) suggest utmost caution when potential exposure of humans to NMs occurs, as long as evidence of their toxicological and genotoxic effect(s) is limited.

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Antibacterial effect of silver nanoparticles on Staphylococcus aureus

Cited by 402 publications

References 30 publications

Biosynthesis, characterization and antibacterial activity of silver nanoparticles from Aspergillus awamori

Biosynthesis, characterization and antibacterial activity of silver nanoparticles from Aspergillus awamori

Synthesis, optimization, and characterization of silver nanoparticles from Acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics

Genotoxicity of Metal Nanoparticles: Focus on In Vivo Studies

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