Antibacterial and antitumour activities of some plants grown in Turkey

Abstract

Screening of antibacterial and antitumour activities of 33 different extracts prepared with three types of solvents (water, ethanol and methanol) was conducted. The extracts were obtained from 11 different plant species grown in Turkey: Eryngium campestre L., Alchemilla mollis (Buser) Rothm., Dorycnium pentaphyllum Scop., Coronilla varia L., Onobrychis oxyodonta Boiss., Fritillaria pontica Wahlenb., Asarum europaeum L., Rhinanthus angustifolius C. C. Gmelin, Doronicum orientale Hoffm., Campanula glomerata L. and Campanula olympica Boiss. Antibacterial activity against six bacteria was evaluated: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pyogenes, Staphylococcus aureus and Staphylococcus epidermidis by using disc diffusion and well diffusion methods. S. aureus and S. epidermidis were most sensitive to the methanolic extract from A. europaeum. S. pyogenes was vulnerable to all used extracts of D. orientale. In addition, ethanolic or methanolic extracts of E. campestre, A. mollis, D. pentaphyllum, C. varia, R. angustifolius, C. glomerata and C. olympica displayed strong antibacterial activity against at least one of the tested gram-negative bacteria. The methanolic extract from R. angustifolius showed a broad-spectrum activity against both gram-positive and gram-negative bacteria. Antitumour activity was evaluated with Agrobacterium-tumefaciens-induced potato disc tumour assay. Best antitumour activity was obtained with the aqueous extract from A. europaeum and methanolic extract from E. campestre (100% and 86% tumour inhibition, respectively).

Fast Identification of Radical Scavengers from Securigera varia by Combining 13C-NMR-Based Dereplication to Bioactivity-Guided Fractionation

“…As a result, one new compound (13) and twelve known flavonoid glycosides together with three nitropropanoylglucopyranoses were isolated including four flavonoids already identified by dereplication (1, 5, 7, and 8), in addition to kaempferol- (14), coronillin (16) and coronarian (15) [10,26] (Figure 3 Figure 3. Structures of compounds 1-16 isolated from the aerial parts of Securigera varia.…”

“…This dereplication approach was completed by further semi-preparative high performance liquid chromatography purification experiments in order to identify unknown or minor active compounds. As a result, one new (13) and twelve known flavonoid glycosides (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) together with three nitropropanoylglucopyranoses (14)(15)(16) were isolated from the aerial parts of Securigera varia. The structures were established by NMR and mass spectrometry as well as by comparison to their spectral data (MS, 1 H-and 13 C-NMR) found in the literature.…”

“…Its chemical composition includes nitropropanoylglucopyranoses, cardenolides, and flavonoids [7][8][9][10]. S. varia is cardiotonic [11] and the seeds of this plant have shown a potential antitumour activity due to their cardenolide content [12,13], whereas the MeOH extract of its aerial parts have demonstrated an antibacterial activity [14]. However, the antioxidant activity of this plant has never been reported.…”

Phenolic Composition, Anti‐Inflammatory, Antioxidant, and Antimicrobial Activities of Alchemilla mollis (Buser) Rothm.

“…Alchemilla mollis ( Buser ) Rothm . is one of the species grown in Turkey and reported to have potent antioxidant activity, but the antimicrobial and anti‐inflammatory activity of this plant has not been documented well . In this study, we have determined antioxidant and antimicrobial activities of hexane, ethyl acetate (AcOEt), methanol (MeOH), butanol (BuOH), water, and 70% methanol (70% MeOH) extracts of A. mollis .…”

Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells

“…Plants of Dorycnium genus produce many biologically active compounds, some of which have been shown to carry as anti-inflammatory, antimicrobial, cytotoxic and antioxidant properties (Bremner et al, 2009;Usta et al, 2014;Stefanović et al, 2015). Agrobacterium tumefaciens with the potato disc method .…”