Antibacterial and antioxidant potential of biosynthesized copper nanoparticles mediated through Cissus arnotiana plant extract

Abstract

Environment friendly methods for the synthesis of copper nanoparticles have become a valuable trend in the current scenario. The utilization of phytochemicals from plant extracts has become a unique technology for the synthesis of nanoparticles, as they possess dual nature of reducing and capping agents to the nanoparticles. In the present investigation we have synthesized copper nanoparticles (CuNPs) using a rare medicinal plant Cissus arnotiana and evaluated their antibacterial activity against gram negative and gram positive bacteria.The morphology and characterization of the synthesized CuNPs were studied and done using UV-Visible spectroscopy at a wavelength range of 350-380nm. XRD studies were performed for analyzing the crystalline nature; SEM and TEM for evaluating the spherical shape within the size range of 60-90nm and AFM was performed to check the surface roughness. The biosynthesized CuNPs showed better antibacterial activity against the gram-negative bacteria, E. coli with an inhibition zone of 22.20±0.16 mm at 75µg/ml. The antioxidant property observed was comparatively equal with the standard antioxidant agent ascorbic acid at a maximum concentration of 40 µg/ ml. This is the first study reported on C. arnotiana mediated biosynthesis of copper nanoparticles, where we believe that the findings can pave way for a new direction in the field of nanotechnology and nanomedicine where there is a significant potential for antibacterial and antioxidant activities. We predict that, these could lead to an exponential increase in the field of biomedical applications, with the utilization of green synthesized CuNPs, due to its remarkable properties. The highest antibacterial property was observed with gram-negative strains mainly, E. coli, due to its thin peptidoglycan layer and electrostatic interactions between the bacterial cell wall and CuNPs surfaces. Hence, CuNPs can be potent therapeutic agents in several biomedical applications, which are yet to be explored in the near future.

Green synthesis of copper nanoparticles using leaf extract of Ageratum houstonianum Mill. and study of their photocatalytic and antibacterial activities

“…However, such peaks were neither assigned with AHLE nor CuCl 2 solution. Similar observation has been reported in previous studies showing the absorption maxima of CuNPs at 384, 350, 310 and 305 nm using the flower extract of Millettia pinnata [55], leaf extracts of Cassia arnotiana [54], Allium cepa, and Azadirachta indica [56], respectively. The complete reduction of Cu + ions to Cu 0 was found after 24 h of incubation.…”

“…After the addition of 20 ml A. houstonianum leaf extract in 80 ml of 3 mM CuCl 2 (2:8 ratio) at room temperature, the solution was continuously stirred for 24 h. The CuCl 2 dissolves in water to give rise Cu 2+ and 2Cl − . The Cu 2+ further dissociates to give zero-valent ions (Cu 0 ) with reaction time by the activity of AHLE phytocompounds, which aggregates to form CuNPs from Cu nuclei [54]. The reaction mixture changed into gleaming greenish color due to the excitation of the surface plasmon resonance (SPR) phenomenon, which indicates the formation of CuNPs [31,32].…”

<p>Phyto-Engineered Gold Nanoparticles (AuNPs) with Potential Antibacterial, Antioxidant, and Wound Healing Activities Under in vitro and in vivo Conditions</p>

“…[9][10][11][12][13] Natural plant sources are gaining vast attention, which can be effectively employed in the preparation of MNPs owing to their easier availability, less toxic behavior and cost-effectiveness. 14,15 The synthesis of MNPs conjugated to various plants such as Cladophora fascicularis, 16 Pinus pinaster, 17 Aerva lanata, 18 Artemisia annua, 19 Hippophae rhamnoides Linn, 20 Eucommia ulmoides, 21 black tea leaf, 22 Averrhoa bilimbi, 23 Salicornia brachiata, 24 Abelmoschus esculentus L, 25 olive leaf, 26 Ipomoea carnea, 27 geranium, 28 and Cissus arnotiana 29 have been documented. Among various types of nanoparticles, gold nanoparticles (AuNPs) have gained greater attraction in antibacterial, antioxidant and wound-healing applications when combined with natural phytoconstituents.…”

Overactivation of the sodium–calcium exchanger and transient receptor potential in anesthesia‐induced malignant hyperthermia

“…TRPV1 is a notable polymodal cell sensor for warmth and other physiological stimuli . In recent times, preclinical information exhibited that anesthetics delivers a depolarizing shift in the voltage reliance of the TRPM8 channel activation . A study explores the results that TRPV1 shares a similar behavior, because VA exposure also produces a depolarizing shift in the voltage dependence of TRPV1 and the fact that identified, VAs as potent activators of this channel in skeletal muscle cells, TRPV1 could be part of malignant hyperthermia (MH) crisis.…”