Antibacterial Amphiphilic Copolymers of Dimethylamino Ethyl Methacrylate and Methyl Methacrylate to Control Biofilm Adhesion for Antifouling Applications

Abstract

Amphiphilic copolymers are recognized as important biomaterials and used as antibacterial agents due to their effective inhibition of bacterial growth. In current study, the amphiphilic copolymers of P(DMAEMA-co-MMA) were synthesized using free radical polymerization by varying the concentrations of hydrophilic monomer 2-dimethylamino ethylmethacrylate (DMAEMA) and hydrophobic monomer methyl methacrylate (MMA) having PDI value of 1.65–1.93. The DMAEMA monomer, through ternary amine with antibacterial property optimized copolymers, P(DMAEMA-co-MMA), compositions to control biofilm adhesion. Antibacterial activity of synthesized copolymers was elucidated against Gram-positive Staphylococcus aureus (ATCC 6538) and Gram-negative Escherchia coli (ATCC 8739) by disk diffusion method, and zones of inhibition were measured. The desired composition that was PDM1 copolymer had shown good zones of inhibition i.e., 19 ± 0.33 mm and 20 ± 0.33 mm for E. coli and S. aureus respectively. The PDM1 and PDM2 have exhibited significant control over bacterial biofilm adhesion as tested by six well plate method. SEM study of bacterial biofilm formation has illustrated that these copolymers act in a similar fashion like cationic biocide. These compositions viz. PDM1 and PDM2, may be useful in development of bioreactors, sensors, surgical equipment and drug delivery devices.

Amphiphilic copolymers of dimethyl aminoethyl methacrylate and methyl methacrylate with controlled hydrophilicity for antialgal activity

“…Traces of GPC distribution was unimodal symmetric that showed the uniformity of the copolymers via free‐radical polymerization. There was slight molecular weight distribution Mw/Mn, <1.4 of all copolymers 31 …”

“…32 This is due to the hydrophobic nature of MMA and the shorter chain of cationic hydrophilic monomer DMAEMA present in the structure of amphiphilic copolymers. 31 In these copolymers, P1 has a greater concentration of hydrophilic content, DMAEMA and has a lower contact angle 67 . In the case of P4 copolymer, the contact angle is 84 due to the greater concentration of hydrophobic content MMA.…”

Past and Current Progress in the Development of Antiviral/Antimicrobial Polymer Coating towards COVID-19 Prevention: A Review

“…A review suggested that an ideal amphiphilic antimicrobial polymer bearing a cationic arm has low molecular weight, a low level of lipophilicity, and is biocompatible towards eukaryotic cells [ 24 ]. An amphiphilic copolymer, P(DMAEMA-co-MMA), as shown in Figure 4 , was successfully synthesized through the free radical polymerization of various concentrations of hydrophilic monomer 2-dimethylamino ethylmethacrylate (DMAEMA) and hydrophobic monomer methyl methacrylate (MMA) [ 58 ]. It was found that the copolymer with a high concentration of DMAEMA showed high biocidal activity against Gram-positive bacteria due to the presence of more amine groups along the DMAEMA chain.…”

Antimicrobial Polymer−Based Assemblies: A Review

“…Most cationic polymers bear amine functions that can be protonated, such as polyethyleneimine (PEI), poly−L−(lysine) (PLL), chitosan, and poly [2−(N,N−dimethylamino)ethyl methacrylate] (PDMAEMA) [ 134 ]. While these polymers have inherent cationic charges, others have been developed by introducing cationic moieties such as aminated cellulose, which, compared to chitosan, represent a novel cationic cellulose derivative with improved mucoadhesive properties as well as sufficient hydration at physiological pH [ 135 ].…”