Antibacterial activity of plant methanolic extracts on a field isolate of &amp;lt;i&amp;gt;Pseudomonas syringae&amp;lt;/i&amp;gt; pvtomato from the casablanca region (Morocco)

Elkhalfi, Bouchra; Essari, Abderrazak; Serrano, Aurelio; Soukri, Abdelaziz

doi:10.4236/abb.2013.74a2001

Cited by 3 publications

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“…However, most of the studies carried out on P. syringae pv. tomato DC 3000 have focused its molecular aspects, while the cellular and physiological aspects have not been yet developed [5].…”

Section: Introductionmentioning

confidence: 99%

“…However, the overall results of this study suggested that Mintha pulegium, Thym vulgaris, Eucalyptus globulus essential oils had potential as a bio-pesticide for the control of bacterial speck disease of tomato. genetics and biotechnology (PGMB) [5]. This strain is characterized by the production of an endotoxin (pyoverdine) [10] and by its multiple resistance to antibiotics (rifampicin) [4].…”

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confidence: 99%

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Evaluation of the Potential of Some Essential Oils in Biological Control against Phytopathogenic Agent Pseudomonas syringae pv. Tomato DC3000 Responsible for the Tomatoes Speck

Sabir¹,

Elkhalfi²,

Errachidi³

et al. 2017

J Plant Pathol Microbiol

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To investigate biological control mains against phytopathogenic agent Pseudomonas syringae pv. tomato DC3000, responsible for bacterial speck, tests on the antibacterial activity of six essential oils were carried out. The essential oils, obtained by hydro distillation, was analyzed by gas chromatography (GC). The determination of the antibacterial activity of the essential oils carried out in-vitro using the well diffusion, micro atmosphere methods and the determination of MIC and CMB. Only essential oils of Cedrus atlantica had a negative activity against the bacterial strain. However, the overall results of this study suggested that Mintha pulegium, Thym vulgaris, Eucalyptus globulus essential oils had potential as a bio-pesticide for the control of bacterial speck disease of tomato. genetics and biotechnology (PGMB) [5]. This strain is characterized by the production of an endotoxin (pyoverdine) [10] and by its multiple resistance to antibiotics (rifampicin) [4]. The P. syringae pv. tomato cultures were grown on King's B and LB with rifampicin (50 g/ml) agar plates for strain verification.

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Evaluation of the Potential of Some Essential Oils in Biological Control against Phytopathogenic Agent Pseudomonas syringae pv. Tomato DC3000 Responsible for the Tomatoes Speck

Sabir¹,

Elkhalfi²,

Errachidi³

et al. 2017

J Plant Pathol Microbiol

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“…tomato DC3000 is an aerobic, rod-shaped, polar, motile and a Gram negative bacteria [1][2][3], which showed a fluorescent pigmentation in King B medium and oxidatively metabolizes glucose. This pathogenic is also characterized by Levan-positive colonies on nutrient agar medium supplemented with sucrose, exhibits positive hypersensitive reaction in the presence of tobacco leaves; it is oxidase, arginine-dehydrolase negative and does not produce pectolytic enzymes (were potato rot-negative) [4,5].…”

Section: Introductionmentioning

confidence: 99%

Identification of an extracellular infection-induced glyceraldehyde-3-phosphate dehydrogenase of the phytopathogenic proteobacterium <i>Pseudomonas syringae</i> pv tomato DC3000

Elkhalfi¹,

Serrano²,

Soukri³

2014

ABB

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According to molecular biology, genomic and proteomic data, the phytopathogenic gamma-proteobacterium Pseudomonas syringae pv. tomato DC3000 produces a number of proteins that may promote infection and draw nutrients from plants. Remarkably, P. syringae DC3000 strain possesses three paralogous gap genes encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) enzymes with different predicted molecular sizes and metabolic functions. As GAPDH was shown to be a virulence factor in other microbial pathogens, in the current study, we analyzed the expression levels of each paralogous gap gene by realtime PCR to understand the actual impact of their protein products on P. syringae virulence. We found that all of them were strongly induced during the infection process. Nevertheless, proteomic analysis of culture supernatants revealed that only Class I GAPDH1 encoded by the gap1 gene was identified as an extracellular protein in infective cells. These results strongly suggest that this GAPDH should play a role in the infective process, including its well-know enzymatic function in the glycolytic metabolic pathway.

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Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial Activity

Derdak

Quinteiro

Sakoui

et al. 2021

The Scientific World Journal

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Recently, the interest in donkey milk has increased considerably because it proved high nutritive and functional values of their ingredients. Its chemical composition is widely studied, but its microbiota, especially lactic acid bacteria, remains less studied. This study focuses on analyzing, isolating, and identifying lactic acid bacteria and evaluating their capacity to produce biomolecules with antibacterial activity. Among 44 strains identified, 43 are Gram-positive, and most are catalase-negative and cocci-shaped. Five strains were selected to evaluate their antibacterial activity against Listeria monocytogenes, Staphylococcus aureus, and Escherichia coli. Different induction methods allowed to amplify the antibacterial effects against these pathogenic strains.

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Antibacterial activity of plant methanolic extracts on a field isolate of <i>Pseudomonas syringae</i> pvtomato from the casablanca region (Morocco)

Cited by 3 publications

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Evaluation of the Potential of Some Essential Oils in Biological Control against Phytopathogenic Agent Pseudomonas syringae pv. Tomato DC3000 Responsible for the Tomatoes Speck

Evaluation of the Potential of Some Essential Oils in Biological Control against Phytopathogenic Agent Pseudomonas syringae pv. Tomato DC3000 Responsible for the Tomatoes Speck

Identification of an extracellular infection-induced glyceraldehyde-3-phosphate dehydrogenase of the phytopathogenic proteobacterium <i>Pseudomonas syringae</i> pv tomato DC3000

Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial Activity

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Antibacterial activity of plant methanolic extracts on a field isolate of &lt;i&gt;Pseudomonas syringae&lt;/i&gt; pvtomato from the casablanca region (Morocco)

Cited by 3 publications

References 0 publications

Evaluation of the Potential of Some Essential Oils in Biological Control against Phytopathogenic Agent Pseudomonas syringae pv. Tomato DC3000 Responsible for the Tomatoes Speck

Evaluation of the Potential of Some Essential Oils in Biological Control against Phytopathogenic Agent Pseudomonas syringae pv. Tomato DC3000 Responsible for the Tomatoes Speck

Identification of an extracellular infection-induced glyceraldehyde-3-phosphate dehydrogenase of the phytopathogenic proteobacterium &lt;i&gt;Pseudomonas syringae&lt;/i&gt; pv tomato DC3000

Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial Activity

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Antibacterial activity of plant methanolic extracts on a field isolate of <i>Pseudomonas syringae</i> pvtomato from the casablanca region (Morocco)

Identification of an extracellular infection-induced glyceraldehyde-3-phosphate dehydrogenase of the phytopathogenic proteobacterium <i>Pseudomonas syringae</i> pv tomato DC3000