Evaluation of the Potential of Some Essential Oils in Biological Control against Phytopathogenic Agent Pseudomonas syringae pv. Tomato DC3000 Responsible for the Tomatoes Speck

Abstract

To investigate biological control mains against phytopathogenic agent Pseudomonas syringae pv. tomato DC3000, responsible for bacterial speck, tests on the antibacterial activity of six essential oils were carried out. The essential oils, obtained by hydro distillation, was analyzed by gas chromatography (GC). The determination of the antibacterial activity of the essential oils carried out in-vitro using the well diffusion, micro atmosphere methods and the determination of MIC and CMB. Only essential oils of Cedrus atlantica had a negative activity against the bacterial strain. However, the overall results of this study suggested that Mintha pulegium, Thym vulgaris, Eucalyptus globulus essential oils had potential as a bio-pesticide for the control of bacterial speck disease of tomato. genetics and biotechnology (PGMB) [5]. This strain is characterized by the production of an endotoxin (pyoverdine) [10] and by its multiple resistance to antibiotics (rifampicin) [4]. The P. syringae pv. tomato cultures were grown on King's B and LB with rifampicin (50 g/ml) agar plates for strain verification.

A Review on Major Constituents of Various Essential Oils and its Application

“…Seed essential oil of Eucalyptus camaldulensis possesses secondary metabolites which could be used for the development of new drug [22]. Phytochemicals and therapeutic potentials of the seed essential oil of E. maculata grown in Nigeria were demonstrated.…”

The Effect of Supplementation with Some Essential Oils on the Mobility and the Vitality of Human Sperm

“…Three Essential oils (Eos) were studied; they were obtained directly from leaves of different vegetal species, as shown in Table 1. Plant material was harvested randomly, then washed, and dried in a well-ventilated place at room temperature for ten days before their use according to the method of Sabir et al [13]. The samples were then isolated from each other's specimens and conserved for extraction.…”

“…Essential oils were obtained by 3.5 h hydrodistillation using the standard Clevenger apparatus according to the method of Sabir et al [13]. The oils were extracted from the distillate with hexane and dehydrated by passing through anhydrous sodium sulfate.…”

“…The oils were extracted from the distillate with hexane and dehydrated by passing through anhydrous sodium sulfate. After filtration, the solvent was removed by distillation under reduced pressure in a rotary evaporator at 35°C, and the pure oils stored in an amber vial kept under refrigeration (4°C), until their use [13]. The nonlethal concentrations of the essential oils were determined by the dilution technique in series according to the method of Mar et al [14].…”

A Green Nanostructured Pesticide to Control Tomato Bacterial Speck Disease

“…Morin, naringenin and phloretin, which are plant flavonoids, affect different Pst strains' virulence, reducing flagellin production and disturbing cell swimming and swarming motility [17]. Ethanolic extract of Magnolia tamaulipana was able to inhibit almost 90% of Pst growth at concentration of 125 ppm, while a strong in vitro antibacterial activity of essential oils extracted from different plants such as mint, thyme and eucalyptus was recorded on Pst, due to the presence of phenolic compounds [18,19]. Chitosan and its derivatives are some of the most studied antimicrobial compounds, the application of which in agriculture is already allowed as resistance inductors [20,21].…”