To investigate biological control mains against phytopathogenic agent Pseudomonas syringae pv. tomato DC3000, responsible for bacterial speck, tests on the antibacterial activity of six essential oils were carried out. The essential oils, obtained by hydro distillation, was analyzed by gas chromatography (GC). The determination of the antibacterial activity of the essential oils carried out in-vitro using the well diffusion, micro atmosphere methods and the determination of MIC and CMB. Only essential oils of Cedrus atlantica had a negative activity against the bacterial strain. However, the overall results of this study suggested that Mintha pulegium, Thym vulgaris, Eucalyptus globulus essential oils had potential as a bio-pesticide for the control of bacterial speck disease of tomato. genetics and biotechnology (PGMB) [5]. This strain is characterized by the production of an endotoxin (pyoverdine) [10] and by its multiple resistance to antibiotics (rifampicin) [4]. The P. syringae pv. tomato cultures were grown on King's B and LB with rifampicin (50 g/ml) agar plates for strain verification.
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