Antibacterial Activity of Endodontic Sealers against Planktonic Bacteria and Bacteria in Biofilms

Kapralos, Vasileios; Koutroulis, Andreas; Ørstavik, Dag; Sunde, Pia Titterud; Valen, Håkon

doi:10.1016/j.joen.2017.08.023

Cited by 74 publications

(92 citation statements)

References 37 publications

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“…Globally, AH Plus showed a reduction in its antimicrobial activity overtime, which is compatible with the results of previous studies 11,25,26) . DCT data indicated that at 24 h after setting, AH Plus achieved a reduction percentage of RLUs close to 80% compared to the control.…”

Section: Discussionsupporting

confidence: 92%

Antibacterial and antibiofilm activity over time of GuttaFlow Bioseal and AH Plus

et al. 2019

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The sealers' antibacterial and antibiofilm activities against Enterococcus faecalis were evaluated by direct contact test (DCT) and confocal laser scanning microscopy (CLSM), respectively, after 1 day, and 1 and 4 weeks of aging. Cell viability was determined by adenosin triphosphate (ATP) assay after DCT. The parameters evaluated for the antibiofilm property were total biovolume and percentage of green cells in E.faecalis biofilms. The data from the bioluminescence ATP assay as well as the total biovolume and green percentage were analyzed by non-parametric tests, Kruskal-Wallis for global comparison and Kolmogorov-Smirnov for each two variables. Results of the DCT and CLSM for all parameters evaluated show that the antimicrobial activity of AH Plus decreased over time, whereas GuttaFlow Bioseal had an opposite property, increasing its antibacterial activity as the material aged.

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Section: Discussionsupporting

confidence: 92%

Antibacterial and antibiofilm activity over time of GuttaFlow Bioseal and AH Plus

et al. 2019

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“…This study evaluated the penetration of a bioceramic sealer in dentinal tubules irradiated by Nd:YAG laser, which was used as a coadjuvant for root canal decontamination. The objective was to combine the benefits of laser microbial reduction with the efficacy of bioceramic sealer [ 27 , 30 , 42 – 48 ]. Since Nd:YAG lasers can melt dentinal surfaces, this study examined whether this bioceramic sealer is capable of penetrating dentinal tubules despite laser irradiation.…”

Section: Discussionmentioning

confidence: 99%

Influence of Nd:YAG laser on the penetration of a bioceramic root canal sealer into dentinal tubules: A confocal analysis

et al. 2018

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ObjectiveThe aim of this in vitro study, is to evaluate the penetration of a bioceramic root canal sealer into dentinal tubules at 3 mm and 5 mm from the apex after Nd:YAG laser irradiation.MethodsForty freshly extracted human mandibular premolars were prepared using Reciproc® and irrigated with 17% ethylenediaminetetraacetic acid (EDTA). Teeth were divided into 4 groups: group 1, obturated with control sealer (AH Plus®); group 2, obturated with bioceramic sealer (Endosequence BC Sealer®); group 3, Nd:YAG laser + control sealer (AH Plus®); and group 4, Nd:YAG laser + bioceramic sealer (Endosequence BC Sealer®). The samples were transversely sectioned 3 mm and 5 mm from the apex and examined using confocal laser scanning microscopy. Two parameters were measured: 1) sealer penetration into dentinal tubules of the root canal and 2) sealer penetration into the perimeter of the root canal walls.ResultsPenetration analysis showed that bioceramic sealer had a higher penetration at depths of 3 and 5 mm than that of the control sealer, regardless of laser use (p <0.05). Perimeter analysis showed that there was no difference between both sealers at a depth of 3 mm (p <0.05), regardless of laser use. At a depth of 5 mm, bioceramic sealer and laser showed a greater perimeter of penetration (p <0.05) than the control sealer.ConclusionThe use of Nd:YAG laser did not compromise the penetration of bioceramic sealer into dentinal tubules of root canals at 3 mm and 5 mm from the apex.

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“…Afterwards, 100 μl of the bacteria containing McFarland 0.5 (1.5 Â 10 8 cfu/ml) were prepared and entered the tubes (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). Then, the tubes were incubated at 37 C for 24 h, where the tube No.…”

Section: Determining Minimum Inhibitory Concentrationmentioning

confidence: 99%

“…However, the problem with these materials is that they show little resistance to the microbial cells that stick to surfaces permanently. The growth of biofilms on food processing equipment and medical devices causes the microbial contamination, and increases the prevalence of food-borne diseases and hospital infections [3]. For example, the bacteria such as Staphylococcus aureus, Escherichia coli, Salmonella enterica, Klebsiella pneumonia, Bacillus subtilis and Acinetobacter baumannii have a strong tendency to biofilm formation, and are found to be highly resistant to disinfectants [4,5].…”

Section: Introductionmentioning

confidence: 99%

A novel synthesis of new antibacterial nanostructures based on Zn-MOF compound: design, characterization and a high performance application

Akbarzadeh

Motaghi

Chauhan³

et al. 2020

Heliyon

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In this study, the novel zinc metal-organic frameworks (MOF) nanostructure has been employed, which was developed using an affordable, environmental friendly, efficient and fast method of ultrasound-assisted reverse micelle (UARM). These nanostructures were identified with various techniques such as FT-IR, XRD, BET, SEM, TG-DSC, TEM and EDS. It was found that the Zn-MOF samples have favorable physicochemical properties. The impact of experimental parameters of the UARM method is effective on the resulting properties, such as high surface area of the products that increases the interactions between the Zn-MOF nanostructure and bacteria.Their antibacterial activities were investigated using diffusion methods in agar and also with dilutions of Zn-MOF samples. Antibiotics (tetracycline and ampicillin) and their anti-biofilm effects were evaluated using microplate method. Obtained results revealed that the Zn-MOF nanostructures have high antibacterial properties which, could be due to the nature of the applied Zn-MOF as well as the optimization process. The Zn-MOF nanostructures could be a novel antibacterial material as biocatalyst processes.

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Antibacterial Activity of Endodontic Sealers against Planktonic Bacteria and Bacteria in Biofilms

Cited by 74 publications

References 37 publications

Antibacterial and antibiofilm activity over time of GuttaFlow Bioseal and AH Plus

Antibacterial and antibiofilm activity over time of GuttaFlow Bioseal and AH Plus

Influence of Nd:YAG laser on the penetration of a bioceramic root canal sealer into dentinal tubules: A confocal analysis

A novel synthesis of new antibacterial nanostructures based on Zn-MOF compound: design, characterization and a high performance application

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