Antibacterial activity of cocoa pod husk phenolic extract against Escherichia coli for food processing

Diniardi, Eka Mustika; Argo, Bambang Dwi; Wibisono, Yusuf

doi:10.1088/1755-1315/475/1/012006

Cited by 6 publications

(12 citation statements)

References 12 publications

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“…In this study, TPC was measured as CPH ethanolic extract was reported to contain polyphenols, including phenolic acids such as salicylic acid, p-hydroxybenzoic acid, protocatechuic acid, and flavonoid, mainly catechin and (epi)gallocatechin. [12] These polyphenol compounds were reported for its ROS scavenging activities, which could interact with the ROS produced by blue light, although the exact mechanism has not been elucidated. [13] In a previous study, TPC value of CPH extract was estimated to be 49.54 ± 3.39 mg GAE/g extract.…”

Section: Discussionmentioning

confidence: 99%

In vitro Cytoprotective Activity Study of Cocoa (Theobroma cacao L.) Pod Husk Ethanolic Extract against Blue Light Irradiation

Adiyanto¹,

Chriscensia²,

Wibowo³

et al. 2023

Pharmacogn. Res.

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Background: Blue light exposure has been shown to induce ROS generation and subsequent inflammatory pathways that lead to cell death, in which antioxidants could counteract this effect. Although regarded as waste, cocoa (Theobroma cacao L.) Pod Husk (CPH) possesses a high polyphenolic contents, which could serve as antioxidants. Objectives: To characterize CPH ethanolic extract based on its antioxidant capacity and observe the cytoprotective ability of CPH in vitro upon blue light exposure. Materials and Methods: CPH ethanolic extract was characterized through total phenolic content, total flavonoid content, and three antioxidant assays, then treated on HaCaT cells, in which cell viability was measured through MTS assay. Results: CPH extract showed high phenolic and flavonoid contents and high antioxidant capacity through DPPH and FRAP assay. CPH extract started to exert cytotoxicity from concentrations of 400 µg/mL and above, while 100 µg/mL and above in AA. Furthermore, CPH extract showed significant cytoprotective effect at 50 µg/mL at 11.92 ± 0.83% cell viability increase, wherein AA failed to protect HaCaT cells at the same concentration at 15.79 ± 0.72% cell viability decrease. Conclusion: CPH could serve as an alternative as blue light protection agent as it was safe to be used at higher concentrations and was able to protect HaCaT cells from blue light irradiation better than AA.

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Section: Discussionmentioning

confidence: 99%

In vitro Cytoprotective Activity Study of Cocoa (Theobroma cacao L.) Pod Husk Ethanolic Extract against Blue Light Irradiation

Adiyanto¹,

Chriscensia²,

Wibowo³

et al. 2023

Pharmacogn. Res.

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“…Total phenolic content (TPC) of the CPHE nanopowder was measured and have previously been reported [32]. The TPC of CPHE nanopowder is 453 mg GAE/g dw extract, and the in vitro study of antibacterial activity of the CPHE phenolic nanopowder showed a zone of inhibition against Eschericia coli at a concentration of 5 mg/mL.…”

Section: Cphe Extract Characteristicsmentioning

confidence: 94%

“…A similar thickness is important because the mechanical properties and mass-transport properties of the composite membranes were investigated from comparable morphological thickness of the membranes. Total phenolic content (TPC) of the CPHE nanopowder was measured and have previously been reported [32]. The TPC of CPHE nanopowder is 453 mg GAE/g dw extract, and the in vitro study of antibacterial activity of the CPHE phenolic nanopowder showed a zone of inhibition against Eschericia coli at a concentration of 5 mg/mL.…”

Section: Composite Membrane Propertiesmentioning

confidence: 94%

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Cacao Pod Husk Extract Phenolic Nanopowder-Impregnated Cellulose Acetate Matrix for Biofouling Control in Membranes

et al. 2021

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The ultrafiltration membrane process is widely used for fruit juice clarification, yet the occurring of fouling promotes a decline in process efficiency. To reduce the fouling potential in the membrane application in food processing, the use of natural phenolic compounds extracted from cocoa pod husk is investigated. The cocoa pod husk extract (CPHE) was prepared in phenolic nanoparticles form and added into the polymer solution at varying concentrations of 0.5 wt%, 0.75 wt%, and 1.0 wt%, respectively. The composite membrane was made of a cellulose acetate polymer using DMF (dimethylformamide) and DMAc (dimethylacetamide) solvents. The highest permeability of 2.34 L m−2 h−1 bar−1 was achieved by 1.0 wt% CPHE/CA prepared with the DMAc solvent. CPHE was found to reduce the amount of Escherichia coli attached to the membranes by 90.5% and 70.8% for membranes prepared with DMF and DMAc, respectively. It is concluded that CPHE can be used to control biofouling in the membrane for food applications.

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“…Avocado seed is a residue rich in tannin polyphenols, phenolic acids, and flavonoids, which are the most representative groups, and other polyphenols, such as: (+)-catechin, (−)-epicatechin, and 3-leucoanthocyanidins, which have demonstrated anti-inflammatory properties [28]. Cocoa pod husks contain polyphenols and phenolic acids, such as protocatechuic acid, p-hydroxybenzoic acid, and salicylic acid-flavonols and flavonoid compounds that allow them to be used as potential food preservatives and antioxidants [29].…”

Section: Determination Of Antioxidant Activity and Polyphenol Content...mentioning

confidence: 99%

Green Synthesis of Antibacterial Silver Nanocolloids with Agroindustrial Waste Extracts, Assisted by LED Light

Cañadas

Gualle

Vizuete

et al. 2022

Colloids and Interfaces

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Herein, the green synthesis of silver nanoparticles (AgNPs), assisted by LED light, using the aqueous extracts of agroindustrial waste products, such as avocado seeds (ASs), cocoa pod husks (CPHs), and orange peels (OPs), is presented. Surface plasmon resonance analysis showed faster and complete NP formation when irradiated with blue LED light. Green and red light irradiation showed non- and limited nanoparticle formation. TEM analyses confirmed the semispherical morphology of the synthesized AgNPs, with the exception of OP–AgNPs, which showed agglomeration during the light irradiation. For AS–AgNPs and CPH–AgNPs, the average particle diameter was about 15 nm. Interestingly, the CPH extract demonstrated faster nanoparticle formation as compared to the AS extract (100 min vs. 250 min irradiation time, respectively). FTIR spectroscopy assessed the involvement of diverse functional groups of the bioactive phytochemicals present in the plant extracts during nanoparticle photobiosynthesis. The antioxidant activity, as determined by ferric reducing antioxidant power (FRAP) assay, varied from 1323.72 µmol TE/mL in the AS aqueous extract to 836.50 µmol TE/mL in the CPH aqueous extract. The total polyphenol content was determined according to the Folin–Ciocalteu procedure; the AS aqueous extract exhibited a higher polyphenol content (1.54 mg GAE/g) than did the CPH aqueous extract (0.948 mg GAE/g). In vitro antibacterial assays revealed that the AS–AgNPs exhibited promising antibacterial properties against pathogenic bacteria (E. Coli), whereas the CPH–AgNPs showed antibacterial activity against S. aureus and E. coli. The green synthesis of AgNPs using AS, CPH, and OP aqueous extracts reported in this work is environmentally friendly and cost-effective, and it paves the way for future studies related to agroindustrial waste valorization for the production of advanced nanomaterials, such as antibacterial AgNPs, for potential biomedical, industrial, and environmental applications.

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Antibacterial activity of cocoa pod husk phenolic extract against Escherichia coli for food processing

Cited by 6 publications

References 12 publications

In vitro Cytoprotective Activity Study of Cocoa (Theobroma cacao L.) Pod Husk Ethanolic Extract against Blue Light Irradiation

In vitro Cytoprotective Activity Study of Cocoa (Theobroma cacao L.) Pod Husk Ethanolic Extract against Blue Light Irradiation

Cacao Pod Husk Extract Phenolic Nanopowder-Impregnated Cellulose Acetate Matrix for Biofouling Control in Membranes

Green Synthesis of Antibacterial Silver Nanocolloids with Agroindustrial Waste Extracts, Assisted by LED Light

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