ANTIBACTERIAL ACTIVITY AND MECHANISM OF ACTION OF RARUGADONG (Dioscorea pyrifolia Kunth.) TUBER EXTRACTS ON Escherichia coli AND Staphylococcus aureus CELL LEAKAGE

Mierza, Vriezka; Rosidah,; Haro, Ginda; Suryanto, Dwi

doi:10.31788/rjc.2020.1335864

Cited by 6 publications

(7 citation statements)

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“…Bacteria isolated from Dengke Naniura produced 1 white oval-shaped bacterial colony and provided a clear zone around the bacteria. Because when MRSA + CaCO3 1% media reacting with lactic acid which was metabolized from lactic acid bacteria, it will form calcium lactate which dissolves in the media and produces a clear zone around the bacterial colony (Haro et al 2020) The characteristics of the LAB are similar to those of the Lactobacillus sp, namely purple Gram-positive bacteria, bacilli-shaped, nonspore, non-motile, catalase-negative, and able to ferment sugar (Hutahaean et al 2019;Ismail et al 2017;Manik et al 2021). 6.00 ± 0.00 b 0.17± 0.00 6.00 ± 0.00 b 0.17± 0.00 6.00 ± 0.00 b 0.18± 0.00 6.00 ± 0.00 b 0.19± 0.00 5% 6.00 ± 0.00 bc 0.17 ± 0.00 6.00 ± 0.00 bc 0.17± 0.00 6.00 ± 0.00 bc 0.18± 0.00 6.00 ± 0.00 bc 0.19± 0.00 10% 9.13 ± 0.51 ab 0.27 ± 0.34 7.87 ± 0.15 ab 0.23± 0.12 7.27 ± 0.25 ab 0.22± 0.09 7.13 ± 0.15 ab 0.23± 0.04 20% 11.57 ± 0.25 ab 0.34± 0.16 10.00 ± 0.10 ab 0.29± 0.08 9.43 ± 0.32 ab 0.28± 0.12 8.47 ± 0.25 ab 0.27± 0.07 40% 14.03 ± 0.15 ab 0.41 ± 0.1 14.07 ± 0.40 ab 0.41± 0.33 14.20 ± 0.53 ab 0.43± 0.20 12.93 ± 0.21 ab 0.42± 0.06 60% 15.70 ± 0.36 ab 0.46 ± 0.24 16.10 ± 0.17 ab 0.47± 0.14 15.17 ± 0.25 ab 0.46± 0.09 14.60 ± 0.40 ab 0.47± 0.11 80% 16.60 ± 0.17 ab 0.49 ± 0.11 17.43 ± 0.71 ab 0.51± 0.59 15.93 ± 0.25 ab 0.48± 0.09 15.77 ± 0.35 ab 0.51± 0.10 100% 17.50 ± 0.10 ab 0.51 ± 0.06 18.33 ± 1.04 ab 0.54± 0.86 17.63 ± 0.12 ab 0.54± 0.04 17.13 ± 0.49 ab 0.56± 0.14 Lacto B…”

Section: Discussionmentioning

confidence: 99%

“…Determination of bacterial cells leakage (wall/membrane) was analyzed by measuring the supernatant at a wavelength of 260 (nucleic acid) and 280 (protein) (Mierza et al 2020). DNA and protein leakage is characterized by an increase in the absorbance of the cell supernatant measured at a wavelength of 260nm and 280nm (Asriani et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Isolation results showed a clear zone around the colony. Then it was taken using ose and cultured repeatedly 3-4 times to get pure bacterial colonies (Haro et al 2020). LAB obtained were examined for bacterial morphology (color, shape, and size), Gram-staining of bacteria, catalase-test, fermentation type, gas formation, H2S, and TSIA test (Hutahaean et al 2019).…”

Section: Isolation and Characterization Of Lactic Acid Bacteriamentioning

confidence: 99%

“…MIC determination against pathogenic bacteria causing diarrhea was carried out by agar diffusion method (wells method) on MRSA and NA bilayer media. Suspension of LAB (10 8 CFU/mL) with various concentrations (5%, 10%, 20%, 40%, 60%, 80%, 100% and Lacto B 100%, % v /v) of 50 µL dropped on each well then left for 15 minutes and incubated at 35 ± 2 o C for 18-24 hours, repeated 3 times (Haro et al 2020). In this study, 0.2% injection of ciprofloxacin antibiotic was used as a positive control and aquadest as a negative control.…”

Section: Determination Of Minimum Inhibitory Concentrationmentioning

confidence: 99%

“…Lactic acid bacteria are a source of probiotics that can be found in fermented foods such as Dengke Naniura. By turning common carp into Dengke Naniura with the addition of Jungga acid, it can kill and inhibit the growth of pathogenic bacteria that cannot survive in acidic pH, while LAB works and survive in acidic pH (Haro et al 2020). Research showed several Lactobacillus (lactic acid bacteria/LAB) antibacterial mechanisms such as competing for receptors, nutrients, and boosting immunity.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria

et al. 2021

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Abstract. Nasri, Harahap U, Silalahi J, Satria D. 2021. Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria. Biodiversitas 22: 3098-3104. Diarrhea is the discharge of liquid or watery stools 3 to 4 times a day caused by a bacterial infection. Treatments for diarrhea are probiotics, which have a beneficial effect on the health of the host such as antibacterial. Traditional Batak Toba fermented food, Dengke Naniura, is a source of probiotics. This study aimed to determine the minimum inhibitory concentration, minimum bactericidal concentration, and leakage of DNA and protein from lactic acid bacteria against pathogens. Isolation of LAB was obtained from Dengke Naniura by pour plate method on deMann Rogosa and Sharpe Agar + CaCO3 1%. In this study, Characterization and analysis of bacterial sequencing used Polymerase Chain Reaction. Determination of MIC used the agar diffusion method. The MBC test used the streaking method which was a stroke from the inhibition zone formed. DNA and protein leakage was measured using spectrophotometry UV-VIS (260nm and 280nm). The isolation results obtained were Lactobacillus fermentum, the characterization showed that the bacteria were Gram-positive, bacilli, non-sporing, catalase-negative, and able to ferment sugar. The MIC determination was obtained at a concentration of 10%v/v with a clear zone diameter. Determination of MBC against pathogens was obtained at different concentrations. The results of DNA and protein leakage showed an increased absorption (260nm and 280nm).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Isolation and Characterization Of Lactic Acid Bacteriamentioning

confidence: 99%

Section: Determination Of Minimum Inhibitory Concentrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria

et al. 2021

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Antibacterial activity of Curcuma domestica Vahl. Rhizome extract using microwave assisted extraction method

Rosidah¹,

Putra²,

Muhammad³

et al. 2023

AIP Conference Proceedings

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Synergy based Extracts of Medicinal Plants: Future Antimicrobials to Combat Multidrug Resistance

Abass

Parveen

Irfan

et al. 2022

CPB

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The use of herbal medicines and supplements in the last thirty years has increased enormously. Herbal medication has demonstrated promising and effective potential against various diseases. Herbal and phytoconstituent medications are gaining popularity globally and many people are adopting herbal remedies to deal with different health issues. The indiscriminate use of antibiotics, due to the development of antimicrobial resistance, poses an unprecedented problem for human civilization. Bacterial infections are difficult to cure because of the propensity of microbes to acquire resistance to a wide range of antimicrobial drugs. New compounds are being explored and quantified for possible antibacterial activity with little or no side effects. Researchers are investigating the range of therapeutic plants mentioned in Unani, Ayurveda, and Siddha around the globe. Known and commonly acclaimed global databases such as PubMed, Research Gate, Science Direct, Google Scholar, were searched using different search strings such as Indian medicinal plants, multidrug resistance (MDR), thin layer chromatography (TLC), antimicrobials, and Synergism were used in diverse combinations to reclaim numerous citations associated with this area. Thus, the current review aims to shed a light on the information of medicinal plants as a potential foundation of herbal drugs and elucidate how synergism and TLC bioautography plays a crucial role in finding antimicrobial compounds.

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ANTIBACTERIAL ACTIVITY AND MECHANISM OF ACTION OF RARUGADONG (Dioscorea pyrifolia Kunth.) TUBER EXTRACTS ON Escherichia coli AND Staphylococcus aureus CELL LEAKAGE

Cited by 6 publications

References 0 publications

Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria

Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria

Antibacterial activity of Curcuma domestica Vahl. Rhizome extract using microwave assisted extraction method

Synergy based Extracts of Medicinal Plants: Future Antimicrobials to Combat Multidrug Resistance

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