Antibacterial Activities of Selected Seaweed and Seagrass from Port Dickson Coastal Water against different Aquaculture Pathogens

Ikhsan, Natrah Fatin Mohd; Zakaria, Muta Harah; Bujang, Japar Sidik; Izzatul, N. M. S.; Ahmad, Shahrul Ainliah Alang

doi:10.17576/jsm-2015-4409-08

Cited by 25 publications

(16 citation statements)

References 20 publications

(14 reference statements)

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“…1 ). In terms of the chosen bioactivity, most of the studies demonstrated an antimacrofouling activity (e.g., Maréchal and Hellio 2011 ; Van Alstyne et al 2014 ) followed by antibacterial (e.g., Silva et al 2013 ; Natrah et al 2015 ) and antimicroalgal activity (e.g. Silkina et al 2012 ; Sun et al 2015 ).…”

Section: Antifouling Compounds From Macroalgaementioning

confidence: 99%

Minireview: algal natural compounds and extracts as antifoulants

2017

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Marine biofouling is a paramount phenomenon in the marine environment and causes serious problems to maritime industries worldwide. Marine algae are known to produce a wide variety of chemical compounds with antibacterial, antifungal, antialgal, and anti-macrofouling properties, inhibiting the settlement and growth of other marine fouling organisms. Significant investigations and progress have been made in this field in the last two decades and several antifouling extracts and compounds have been isolated from micro- and macroalgae. In this minireview, we have summarized and evaluated antifouling compounds isolated and identified from macroalgae and microalgae between January 2010 and June 2016. Future directions for their commercialization through metabolic engineering and industrial scale up have been discussed. Upon comparing biogeographical regions, investigations from Southeast Asian waters were found to be rather scarce. Thus, we have also discussed the need to conduct more chemical ecology based research in relatively less explored areas with high algal biodiversity like Southeast Asia.

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Section: Antifouling Compounds From Macroalgaementioning

confidence: 99%

Minireview: algal natural compounds and extracts as antifoulants

2017

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“…The bioactive properties of the seagrass species selected in this work are well known, and have been reported in detail by several authors [ 45 – 47 ]. However, until now attention has mainly focused on the antimicrobial activity of seagrass extracts, which, through disk diffusion assays, were investigated not in their capacity as biofilm-forming microorganisms but in their planktonic state.…”

Section: Discussionmentioning

confidence: 99%

“…Using lethal concentrations, Umamaheshwari et al [ 46 ] reported the antibacterial activity of H. ovalis and H. pinifolia extracts, obtained using different solvents, against different microbial strains, recording maximum antibacterial activity by the ethanol extract of H. pinifolia . Instead, Choi et al [ 48 ] reported the antimicrobial properties of Zostera marina methanol extract and its organic solvent fractions on three human skin pathogens ( Staphylococcus aureus , S. epidermidis and C. albicans ), and Natrah et al [ 47 ] reported the antibacterial properties of methanol extracts of E. acoroides and other seagrass and seaweed species on different aquaculture pathogens ( Aeromonas hydrophila , Vibrio alginolyticus , V. parahaemolyticus , V. anguillarum and others).…”

Section: Discussionmentioning

confidence: 99%

Hindering the formation and promoting the dispersion of medical biofilms: non-lethal effects of seagrass extracts

Vincenti

Glasenapp

Cattò

et al. 2018

BMC Complement Altern Med

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BackgroundBiofilms have great significance in healthcare-associated infections owing to their inherent tolerance and resistance to antimicrobial therapies. New approaches to prevent and treat unwanted biofilms are urgently required. To this end, three seagrass species (Enhalus acoroides, Halophila ovalis and Halodule pinifolia) collected in Vietnam and in India were investigated for their effects in mediating non-lethal interactions on sessile bacterial (Escherichia coli) and fungal (Candida albicans) cultures. The present study was focused on anti-biofilm activities of seagrass extracts, without killing cells.MethodsMethanolic extracts were characterized, and major compounds were identified by MS/MS analysis. The antibiofilm properties of the seagrass extracts were tested at sub-lethal concentrations by using microtiter plate adhesion assay. The performance of the most promising extract was further investigated in elegant bioreactors to reproduce mature biofilms both at the solid/liquid and the solid/air interfaces. Dispersion and bioluminescent assays were carried out to decipher the mode of action of the bioactive extract.ResultsIt was shown that up to 100 ppm of crude extracts did not adversely affect microbial growth, nor do they act as a carbon and energy source for the selected microorganisms. Seagrass extracts appear to be more effective in deterring microbial adhesion on hydrophobic surfaces than on hydrophilic. The results revealed that non-lethal concentrations of E. acoroides leaf extract: i) reduce bacterial and fungal coverage by 60.9 and 73.9%, respectively; ii) affect bacterial biofilm maturation and promote dispersion, up to 70%, in fungal biofilm; iii) increase luminescence in Vibrio harveyi by 25.8%. The characterization of methanolic extracts showed the unique profile of the E. acoroides leaf extract.ConclusionsE. acoroides leaf extract proved to be the most promising extract among those tested. Indeed, the selected non-lethal concentrations of E. acoroides leaf extract were found to exert an antibiofilm effect on C. albicans and E. coli biofilm in the first phase of biofilm genesis, opening up the possibility of developing preventive strategies to hinder the adhesion of microbial cells to surfaces. The leaf extract also affected the dispersion and maturation steps in C. albicans and E. coli respectively, suggesting an important role in cell signaling processes.

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“…al., 2016) Direndam kertas cakram dalam larutan sampel selama 5 menit (Tari, 1999). (Oktavianus, 2013;Natrah, et al, 2015).…”

Section: Pendahuluanunclassified

Sensitivitas Antibakteria Dari Tanaman Caulerpa sp. Dan Enteromorpha sp. Terhadap Bakteri Vibrio alginolyticus

Mazni

Ramses

Rahmi

et al. 2018

SIM-BIO

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Tanaman Caulerpa lentillifera, Caulerpa racemosa dan Enteromorpha compressa tergolong tanaman makroalga yang hidup di perairan laut dangkal dan banyak dijumpai di perairan Batam, Kepulauan Riau. Spesies makroalga berpotensi memiliki senyawa bioaktif sebagai antibakteri seperti spesies C.lentillifera, C.racemosa dan E.compressa. Tujuan penelitian ini untuk mengetahui adanya respon Sensitivitas Antibakteria dari Tanaman Caulerpa sp. dan Enteromorpha sp. Terhadap Bakteri Vibrio alginolyticus diaplikasikan dalam Pembuatan Media Pembelajaran Accordion Book Pada Materi Bioteknologi. Penelitian ini dilaksanakan pada bulan Februari-April 2018 di Laboratorium Penguji Kesehatan Ikan dan Lingkungan Balai Perikanan Budidaya Laut Batam (BPBL). Sampel makroalga diperoleh dari perairan Pulau Sarang, Kecamatan Belakang Padang, Kota Batam. Isolat murni bakteri berasal dari BPBL Batam. Metode yang digunakan dalam penelitian ini adalah eksperimental laboratoris. Uji sensitivitas terhadap bakteri V. alginolyticus dilakukan dengan metode difusi cakram Kirby-Bauer. Kertas cakram yang digunakan berukuran 5 mm. Pengukuran diameter zona hambat dilakukan pada masa inkubasi 6,12,18,24 jam. Analisis data menggunakan teknik analisis deskriptif. Berdasarkan hasil penelitian bahwa adanya respon sensitivitas antibakteria dari tanaman C.lentillifera, C.racemosa dan E.compressa terhadap bakteri V.alginolyticus pada uji maserasi, tetapi tidak untuk uji langsung dan uji ekstrak basah. Pada metode maserasi zona hambat dari tanaman C.racemosa sebesar 7 mm, C.lentillifera sebesar 6,6 mm, dan E.compressa sebesar 6,3 mm termasuk kategori “Sedang” yaitu 5-10 mm.

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Antibacterial Activities of Selected Seaweed and Seagrass from Port Dickson Coastal Water against different Aquaculture Pathogens

Abstract: Eight seaweed species in Teluk

Cited by 25 publications

References 20 publications

Minireview: algal natural compounds and extracts as antifoulants

Minireview: algal natural compounds and extracts as antifoulants

Hindering the formation and promoting the dispersion of medical biofilms: non-lethal effects of seagrass extracts

Sensitivitas Antibakteria Dari Tanaman Caulerpa sp. Dan Enteromorpha sp. Terhadap Bakteri Vibrio alginolyticus

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