Antibacterial action of chitosan-arginine against Escherichia coli O157 in chicken juice

Lahmer, Rabya A.; Williams, A. Prysor; Townsend, Stacy M.; Baker, Shenda M.; Jones, Davey L.

doi:10.1016/j.foodcont.2012.01.039

Cited by 31 publications

(22 citation statements)

References 20 publications

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“…According to zone of inhibition the CuO-NPs as seen in Table 1 exhibited more inhibitory activity towards gram negative than gram positive bacteria as seen in (Fig.7, Table 1 ) because there is a difference in structure of the bacterial cell wall as gram negative cell wall consists of single peptidoglycan layer while gram positive cell wall have several peptidoglycan layers 12 , in contrast to J. Emima Jeronsia et al, who observed that the Grampositive bacterial strains have higher sensitivity to CuO-NPs than Gramnegative 44 . In our research we demonstrated the antibacterial activity of chitosan towards Gram positive and gram-negative bacteria as recorded in Table.2 and agreed with many investigation recorded that chitosan shows antibacterial activity towards both gram-negative and Gram positive bacteria due to the nature of chitosan surface of as polycation of high charge density which tightly attached and absorbed onto the surface of bacterial membrane to disrupt the cell membrane, thus kill bacterial cells 27,28,45,46 . Also Chitosan can form various chemical bonds with metal particles and prevents agglomeration, thus increasing the stability of the NPs 26 .…”

supporting

confidence: 86%

Synthesis of Copper-Chitosan Nanocomposites and its Application in Treatment of Local Pathogenic Isolates Bacteria

Syame¹,

Mohamed²,

Mahmoud³

et al. 2017

Orient. J. Chem

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Development of nanotechnology, nanoparticles based product and its application is generating interest of many researchers due to its promising biological achievement. However, it is well known that inorganic nanomaterials are good antimicrobial agents. Among the various nanoparticles, metal nanoparticles as copper assume special importance due to its low cost and easy availability. In this study, the green synthesis method as eco-friendly approach is used to produce biologically copper oxide nanoparticles from Ficus carica leaf extract, stable CuO NPs were formed. The synthesized nanoparticles is characterized through the UV-Vis Spectrophotometer as it found to be 437 nm, Transmission Electron microscopy (TEM) investigated particle sizes in the range 51-62 nm and typical XRD patterns of the formed CuO NPs with high phase purity were obtained. Chitosan stabilizer as naturally occurring polymers was added to the prepared copper nanoparticles in different amounts to obtain Chitosan-CuO with different CuO percentage for longterm stability, for prevention the agglomeration of nanoparticles and enhancing their antibacterial efficacy. FTIR spectroscopy analysis was performed to copperoxide nanoparticle, chitosan, copperoxide chitosan composite to confirm that CuO nanoparticle was mixed with polymer. The antibacterial efficacy of chitosan, copper nanoparticles alone was studied against 22 bacterial pathogen like Coaggulase +ve S. aureus, methicillin-resistant Coaggulase -ve S. aureus, Klebsiella pneumonia as gram positive bacteria, Escherichia coli, e.coli o157 Salmonella typhi, Pseudomonas aeruginosa as gram negative bacteria that showed antibacterial activity against Gram positive as well as Gram negative bacteria. Antimicrobial activities of polymer/metal composites (Co oxide-chitosan nanoparticle) is studied against the same bacterial pathogen. The effect of the prepared Chitosan-CuO composite on ultrastructure of bacterial cells were evaluated by scanning electron microscopy (SEM), it was found that the antibacterial activity of Cu-chitosan nanoparticle composite is more greater than antibacterial activity of copper nanoparticles and chitosan alone that indicate the addition of chitosan stabilizer enhance at great extent the antimicrobial activity of CuO NPs.

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supporting

confidence: 86%

Synthesis of Copper-Chitosan Nanocomposites and its Application in Treatment of Local Pathogenic Isolates Bacteria

Syame¹,

Mohamed²,

Mahmoud³

et al. 2017

Orient. J. Chem

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“…This supports the findings of Lahmer et al . () that also showed that Ch‐arg was less active against nongrowing cells at 4 °C in comparison with those more metabolically active being held at 20 °C.…”

Section: Discussionmentioning

confidence: 97%

Susceptibility of Escherichia coli O157 to chitosan‐arginine in beef liquid purge is affected by bacterial cell growth phase

Lahmer

Jones

Townsend

et al. 2013

Int J of Food Sci Tech

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Summary Here, we evaluated the impact of bacterial growth stage on the effect of chitosan‐arginine (Ch‐arg) on Escherichia coli O157:H7 cell numbers and metabolic activity within contaminated beef juice held at room temperature. Using a lux‐marked metabolic reporter strain of E. coli O157:H7, the results showed that Ch‐arg was most bioactive against cells in the lag phase and exponential phase. In comparison, there was a reduced, although still significant, inhibitory effect of Ch‐arg on the viability and metabolic activity of E. coli O157 held in stationary phase. Ch‐arg reduced, but did not eliminate E. coli O157 growth in the meat juice over 48 h. Based on the evidence presented here and elsewhere, we conclude that Ch‐arg can limit the growth and activity of food spoilage bacteria; however, it cannot completely eliminate bacterial contaminants originally present. Ch‐arg should therefore be viewed as a potentially protective measure rather than a biocidal agent that completely eliminates the risk of pathogen transfer in the food chain.

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“…Pengujian aktivitas antibakteri dilakukan dengan menggunakan metode OD λmax 600 nm (Sezonov et al, 2007dan Matlock et al, 2011 terhadap bakteri Escherichia coli ATCC 25922, meliputi 3 tahap sebagai berikut: a. Persiapan Media Media pertumbuhan bakteri Escherichia coli yang digunakan adalah Luria Bertani (LB) (Lahmer et al, 2012;Matlock et al, 2011;Sezonov et al, 2007dan Tang et al, 2010 Pengujian antibakteri dilakukan dengan mengukur adsorbansi larutan sampel jam ke-0, 3, 6, 9, 12, dan 24 menggunakan spektrofotometer UV-Vis pada λmax 600 nm secara duplo (Sezonov et al, 2007dan Matlock et al, 2011. Pada uji saponin timbulnya busa pada uji Forth menunjukkan adanya glikosida yang mempunyai kemampuan membentuk buih dalam air yang terhidrolisis menjadi glukosa dan senyawa lainnya (Marliana, 2005 (Marliana, 2005).…”

Section: Uji Antibakteriunclassified

ANTIBACTERIAL ACTIVITY TEST OF EXTRACT NYAMPLUNG (Calophyllum inophyllum Linn.) LEAVES

2016

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ABSTRAKTelah dilakukan skrining fitokimia dan uji aktivitas antibakteri dari ekstrak etanol daun nyamplung (Callophylum inophyllum Linn.) dari Indonesia terhadap pertumbuhan bakteri Escherichia coli. Ekstraksi dilakukan dengan ekstraksi soklet menggunakan pelarut etanol. Uji aktivitas antibakteri menggunakan metode Optical Density pada λmax 600 nm. Hasil pemeriksaan skrining fitokimia ekstrak etanol daun nyamplung menunjukan adanya senyawa golongan flavonoid, saponin, tanin dan fenol serta triterpenoid. Uji aktivitas antibakteri menunjukkan aktivitas antibakteri ekstrak etanol paling besar pada waktu inkubasi 3 jam dengan persentase inhibisi 59,03 %.Kata Kunci: aktivitas antibakteri, daun nyamplung, senyawa metabolit sekunder ABSTRACT Phytochemical screening and antibacterial activity from ethanol extract of nyamplung (Callophylum inophyllum Linn.) leaves from Indonesia against Escherichia coli has been done. The extraction was done by soklet extraction using ethanol. Antibacterial activity test using the Optical Density method at λmax 600 nm. The result of phytochemical screening from ethanol extract showed that there were flavonoid compounds, saponins, tannins, phenols and triterpenoids. The result of antibacterial activity test showed that the optimum activity occurs at incubation time of 3 hours with percentase inhibition persentase i.e. 59.03 %.

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Antibacterial action of chitosan-arginine against Escherichia coli O157 in chicken juice

Cited by 31 publications

References 20 publications

Synthesis of Copper-Chitosan Nanocomposites and its Application in Treatment of Local Pathogenic Isolates Bacteria

Synthesis of Copper-Chitosan Nanocomposites and its Application in Treatment of Local Pathogenic Isolates Bacteria

Susceptibility of Escherichia coli O157 to chitosan‐arginine in beef liquid purge is affected by bacterial cell growth phase

ANTIBACTERIAL ACTIVITY TEST OF EXTRACT NYAMPLUNG (Calophyllum inophyllum Linn.) LEAVES

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