Synthesis of Copper-Chitosan Nanocomposites and its Application in Treatment of Local Pathogenic Isolates Bacteria

Abstract

Development of nanotechnology, nanoparticles based product and its application is generating interest of many researchers due to its promising biological achievement. However, it is well known that inorganic nanomaterials are good antimicrobial agents. Among the various nanoparticles, metal nanoparticles as copper assume special importance due to its low cost and easy availability. In this study, the green synthesis method as eco-friendly approach is used to produce biologically copper oxide nanoparticles from Ficus carica leaf extract, stable CuO NPs were formed. The synthesized nanoparticles is characterized through the UV-Vis Spectrophotometer as it found to be 437 nm, Transmission Electron microscopy (TEM) investigated particle sizes in the range 51-62 nm and typical XRD patterns of the formed CuO NPs with high phase purity were obtained. Chitosan stabilizer as naturally occurring polymers was added to the prepared copper nanoparticles in different amounts to obtain Chitosan-CuO with different CuO percentage for longterm stability, for prevention the agglomeration of nanoparticles and enhancing their antibacterial efficacy. FTIR spectroscopy analysis was performed to copperoxide nanoparticle, chitosan, copperoxide chitosan composite to confirm that CuO nanoparticle was mixed with polymer. The antibacterial efficacy of chitosan, copper nanoparticles alone was studied against 22 bacterial pathogen like Coaggulase +ve S. aureus, methicillin-resistant Coaggulase -ve S. aureus, Klebsiella pneumonia as gram positive bacteria, Escherichia coli, e.coli o157 Salmonella typhi, Pseudomonas aeruginosa as gram negative bacteria that showed antibacterial activity against Gram positive as well as Gram negative bacteria. Antimicrobial activities of polymer/metal composites (Co oxide-chitosan nanoparticle) is studied against the same bacterial pathogen. The effect of the prepared Chitosan-CuO composite on ultrastructure of bacterial cells were evaluated by scanning electron microscopy (SEM), it was found that the antibacterial activity of Cu-chitosan nanoparticle composite is more greater than antibacterial activity of copper nanoparticles and chitosan alone that indicate the addition of chitosan stabilizer enhance at great extent the antimicrobial activity of CuO NPs.

Preparation and characterization of ascorbic acid-mediated chitosan–copper oxide nanocomposite for anti-microbial, sporicidal and biofilm-inhibitory activity

“…Ascorbic acid act as a reducing and capping agent to impart stability to the nanocomposite [26]. The antimicrobial property of metal/polymer nanocomposites may be due to the adsorption of bacteria and causes disruption of the membrane [27]. Similar to our results, the antibacterial activity of hybrid chitosan-cerium oxide nanoparticles was recorded against E. coli and B. subtilis [28].…”

Evaluation of the Mechanical, Physical, and Anti-Fungal Properties of Flax Laboratory Papersheets with the Nanoparticles Treatment

“…It should be noted that from the literature, most of the published works which chose concentrations of NPs between 1% and 3% achieved greater modifications in the required chemical and mechanical properties of chitosan and Paraloid [29,[45][46][47]. Paraloid B-72 (70 Ethyl Methacrylate (EMA)/30MA) and Chitosan were added at 4% as a constant amount when making the composite treatment.…”

Punica granatum flower extract mediated synthesis of Copper oxide Nanoparticle and evolution of its antibacterial activity

“…Primary and secondary alcohols showed bands at 1043 cm -1 as well as the peaks around 1447 cm -1 attribute to aliphatic C-H bending modes. The major peak was observed in CuONPs to be 546 cm -1 was due to stretching of Cu-O bond [26] . In addition the disappearance of γ C=O stretching vibration of the α,β-unsaturated ketone at 1716 cm [27].…”

“…Figure.3 indicates the UV-Vis absorption spectrum of copper oxide nanoparticles and Punica grantum flower extract. The synthesized CuO NPs showed an absorption maximum at 442 nm due to surface plasmon resonance of CuO NPs [26]. …”