Preparation and characterization of ascorbic acid-mediated chitosan–copper oxide nanocomposite for anti-microbial, sporicidal and biofilm-inhibitory activity

Abstract

The chitosan-copper oxide (Chi-CuO) biopolymer nanocomposites were synthesized by a simple green chemistry method using ascorbic acid as a reducing and capping agent. The intense peak around 300 nm was observed in the UV-visible spectrum indicating the formation of CuO nanoparticles. The prepared Chi-CuO nanocomposites were characterized using energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), X-ray diffraction spectroscopy (XRD), and Fourier transform-infrared spectroscopy (FT-IR). SEM and XRD pattern showed cubic shape for Chi-CuO nanocomposites with average crystalline size of 17 nm, as calculated using Debye-Scherrer's formula. The FT-IR spectral studies showed the Cu-O bond formation with chitosan to form nanocomposites. Synthesized nanocomposites showed significant anti-microbial activity against Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli and Penicillium notatum, assayed using the agar well diffusion method. It also showed sporicidal activity against B. subtilis and exhibited effective biofilminhibitory activity against B. subtilis (69%/100 μg/mL) and P. aeruginosa (63%/100 μg/mL).

A Comprehensive Review of the Development of Carbohydrate Macromolecules and Copper Oxide Nanocomposite Films in Food Nanopackaging

“…Logpriya et al [210] presented similar results to Farhoudian et al ey examined the antimicrobial activity of chi-CuO at 25, 50, and 100 μL.…”

“…Logpriya et al [ 210 ] presented similar results to Farhoudian et al They examined the antimicrobial activity of chi-CuO at 25, 50, and 100 μ L. They showed the IZs for E. coli at 25 μ Land 50 μ L to be 6 mm, and at 100 μ L to be 8 mm. The IZs for B. subtilis , P. aeruginosa , and P. notatum at 25, 50, and 100 μ L concentrations were 6.5, 7, 8 mm; 6, 6.5, 8 mm; and 0, 10, 10 mm, respectively ( Table 5 ).…”

Effect of chitosan coating on polypropylene fibers on the deposition of copper ions

“…28 Recently, Logpriya et al has developed a chitosancopper oxide composite. 29 In that work, the antibacterial activity of the obtained composite was demonstrated. However, this composite was created without any substrate, which limits the possibilities for its real application.…”

“…Therefore, an important task to be solved is to obtain a chitosan‐copper containing composite on a substrate. In addition, the chitosan solution in Reference [29] was obtained using acetic acid, which can negatively affect the overall biocompatibility of the composite. The creation of a substrate with chitosan coating using solutions of carbonic acid instead of acetic acid should increase biocompatibility of the composite.…”

Metal Based Nanoparticles, a Novel Approach to Control Biofilm: Review

“…Widyńska et al, (2018) found silver nanoparticls to have stronger antibiofilm effect than the Cu-NPs in all concentration. Logpriya et al, (2018) studied antibiofilm effect of chitosan-CuO nano particles and found to have 63% reduction at 100ug/ml against P. aeruginosa. Beltrán et al, (2019) Wang et al, (2019) studied glycol modified Cu9S8 NPs against implant related biofilm.…”