ANTIBACTERIAL ACTIVITY TEST OF EXTRACT NYAMPLUNG (Calophyllum inophyllum Linn.) LEAVES

Abstract

ABSTRAKTelah dilakukan skrining fitokimia dan uji aktivitas antibakteri dari ekstrak etanol daun nyamplung (Callophylum inophyllum Linn.) dari Indonesia terhadap pertumbuhan bakteri Escherichia coli. Ekstraksi dilakukan dengan ekstraksi soklet menggunakan pelarut etanol. Uji aktivitas antibakteri menggunakan metode Optical Density pada λmax 600 nm. Hasil pemeriksaan skrining fitokimia ekstrak etanol daun nyamplung menunjukan adanya senyawa golongan flavonoid, saponin, tanin dan fenol serta triterpenoid. Uji aktivitas antibakteri menunjukkan aktivitas antibakteri ekstrak etanol paling besar pada waktu inkubasi 3 jam dengan persentase inhibisi 59,03 %.Kata Kunci: aktivitas antibakteri, daun nyamplung, senyawa metabolit sekunder ABSTRACT Phytochemical screening and antibacterial activity from ethanol extract of nyamplung (Callophylum inophyllum Linn.) leaves from Indonesia against Escherichia coli has been done. The extraction was done by soklet extraction using ethanol. Antibacterial activity test using the Optical Density method at λmax 600 nm. The result of phytochemical screening from ethanol extract showed that there were flavonoid compounds, saponins, tannins, phenols and triterpenoids. The result of antibacterial activity test showed that the optimum activity occurs at incubation time of 3 hours with percentase inhibition persentase i.e. 59.03 %.