Anti-Thrombotic and Anti-Inflammatory Activities of Protopine

Saeed, Sheikh A.; Gilani, Anwarul Hassan; Majoo, R.U.; Shah, Bukhtiar H.

doi:10.1006/phrs.1997.0195

Cited by 81 publications

(51 citation statements)

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“…However, a direct effect of C. majus on cardiac tissue has not yet been confirmed in research studies. The sole possible indirect correlation is supported by the antiplatelet effect of sanguinarine and protopine [8,9] which might be useful in cardiovascular diseases.…”

mentioning

confidence: 86%

Chelidonium majus – an Integrative Review: Traditional Knowledge versus Modern Findings

Gîlcă

Găman

Panait

et al. 2010

Forsch Komplementmed

114

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Chelidonium majus L. (family Papaveraceae), or greater celandine, is an important plant in western phytotherapy and in traditional Chinese medicine. Crude extracts of C. majus as well as purified compounds derived from it exhibit a broad spectrum of biological activities (antiinflammatory, antimicrobial, antitumoral, analgesic, hepatoprotective) that support some of the traditional uses of C. majus. However, herbal medicine also claims that this plant has several important properties which have not yet been scientifically studied: C. majus is supposed to have diuretic, antitussive and eye-regenerative effects. On the other hand, C. majus also has scientifically proven effects, e.g. anti-osteoporotic activity and radioprotection, which are not mentioned in traditional sources. Moreover, recent controversy about the hepatoprotective versus hepatotoxic effects of Chelidonium majus has renewed the interest of the medical community in this plant. This review is intended to integrate traditional ethno-medical knowledge and modern scientific findings about C. majus in order to promote understanding of its therapeutic actions as well as its toxic potential.

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mentioning

confidence: 86%

Chelidonium majus – an Integrative Review: Traditional Knowledge versus Modern Findings

Gîlcă

Găman

Panait

et al. 2010

Forsch Komplementmed

114

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“…A similar mechanism in the synergism between various platelet agonists is thought to be due to the activation of Ca 2þ signaling cascade. A rise in intracellular Ca 2þ concentration, induced by the first agonist, primes platelets for an enhanced functional response to the second agonist (Saeed et al, 1997(Saeed et al, , 2004. A similar effect may also explain, at least partly, the mechanism of action of aand b-amyrin as platelet inhibitors.…”

Section: Discussionmentioning

confidence: 99%

Antiplatelet Activity ofα.- andβ.-Amyrin, Isomeric Mixture fromProtium heptaphyllum.

Aragão

Carneiro

Júnior

et al. 2007

Pharmaceutical Biology

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A mixture of triterpenes named a-and b-amyrin (AMI), isolated from the Brazilian medicinal herb Protium hetaphyllum (Aubl) March (Burseraceae), was evaluated for the ability to inhibit aggregation of human platelets induced by adenosine 5 0 -diphosphate (ADP, 1.5 and 3 mM), collagen, and arachidonic acid (AA) in vitro. The results showed that AMI significantly inhibited platelet aggregation (40, 64, and 60%) in the assay carried out with ADP (3 mM) as agonist, at the doses of 100, 150, and 200 mM, respectively. In the presence of a lower ADP concentration (1.5 mM), a 3-time higher percentage of inhibition (32%) was observed with AMI 50 mM, as compared to that seen with 3.0 mM ADP. In the test using collagen (10 mg=mL) as agonist, AMI (50, 100, and 150 mM) inhibited aggregation by 26, 47, and 39%, respectively, while in the presence of the arachidonic acid (150 mM) at the doses of 50, 100, 150, and 200 mM, it inhibited platelet aggregation by 20, 21, 25, and 27%, respectively. The lowest IC 50 value for the AMI inhibitory effect was observed with collagen (90.0 mM), followed by ADP (117.9 mM) and arachidonic acid (181.4 mM). With ADP as agonist, the anti-aggregant effect of the acetylsalicylic acid (ASA) was potentiated by AMI but not by dipyridamole. No potentiation was observed after the combination of ASA and AMI with collagen or arachidonic acid as agonists. Our results indicated that AMI possesses a platelet anti-aggregant activity in a concentration-dependent manner and probably acts on a biochemical pathway common to all the agonists tested.

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“…Protopine also shows anticancer activity by inhibiting NO production in RAW264.7 macrophage cells [24]. Moreover, protopine also exhibited anticholinesterase and antithrombotic activities [25].…”

Section: Liquid Chromatography 297mentioning

confidence: 98%

Simultaneous determination and pharmacokinetics of five alkaloids in rat plasma by ultra high performance liquid chromatography with tandem mass spectrometry after the oral administration of Corydalis bungeana Turcz extract

Liu

Wang

et al. 2015

J of Separation Science

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Pharmacokinetic studies were conducted on rats for protopine, corynoline, 7'-(3',4'-dihydroxyphenyl)-N-[(4-methoxyphenyl)-ethyl]propenamide, acetylcorynoline, and 8-oxocorynoline, five main active components from Corydalis bungeana Turcz (C. bungeana Turcz). An ultra high performance liquid chromatography with tandem mass spectrometry method has been developed and validated for the simultaneous determination of the pharmacokinetic characteristics of these components in rat plasma. Chromatographic separation was achieved on an Agilent SB-C18 column (1.8 μm, 150 × 2.1 mm) using a gradient elution program with a mobile phase consisting of acetonitrile and water containing 0.1% acetic acid at a flow rate of 0.3 mL/min. The analytes were detected in the multiple reaction monitoring mode with positive electrospray ionization. Lower limits of quantification were >0.680 ng/mL and matrix effects ranged from 91.26 to 100.38%. The mean extraction recoveries of quality control samples were less than 79.32%, and the precision and accuracy were within the acceptable limits. All analytes were proven to be stable during sample storage and analysis procedures. The validated method was successfully applied to the pharmacokinetic study of five alkaloid components after oral administration of C. bungeana Turcz extract to rats. The obtained results may be helpful to reveal the mechanism of action and to guide the clinical application of C. bungeana Turcz.

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Anti-Thrombotic and Anti-Inflammatory Activities of Protopine

Cited by 81 publications

References 0 publications

Chelidonium majus – an Integrative Review: Traditional Knowledge versus Modern Findings

Chelidonium majus – an Integrative Review: Traditional Knowledge versus Modern Findings

Antiplatelet Activity ofα.- andβ.-Amyrin, Isomeric Mixture fromProtium heptaphyllum.

Simultaneous determination and pharmacokinetics of five alkaloids in rat plasma by ultra high performance liquid chromatography with tandem mass spectrometry after the oral administration of Corydalis bungeana Turcz extract

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