Antiplatelet Activity ofα.- andβ.-Amyrin, Isomeric Mixture fromProtium heptaphyllum.

Aragão, Gislei Frota; Carneiro, Lyvia Maria Vasconcelos; Júnior, Antônio P. F.; Bandeira, Paulo Nogueira; Lemos, Telma L. G.; Viana, Glauce Socorro de Barros

doi:10.1080/13880200701212916

Cited by 21 publications

(7 citation statements)

References 31 publications

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“…Alpha and beta amyrin: in vitro assays have shown that amyrins inhibited platelet aggregation induced by different agonists, 29 in particular β-amyrin inhibited collagen induced aggregation with IC50 lower than Aspirin. 30 α-Amyrin, has also shown to be a competitive inhibitor of PKC.…”

Section: Discussionmentioning

confidence: 99%

Buddleja globosa (matico) prevents collagen-induced platelet activation by decreasing phospholipase C-gamma 2 and protein kinase C phosphorylation signaling

Fuentes

Sepúlveda

Alarcón

et al. 2018

Journal of Traditional and Complementary Medicine

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Platelets play a key role in thrombosis and cardiovascular diseases. Medicinal plants could be one of the most important factors that influence risks for platelet activation. Buddleja globosa (known as “matico”) is a medicinal plant with many biological activities. The high content of polyphenols suggest that matico could have antiplatelet activity. The present study was aimed at evaluating mechanisms of antiplatelet action of an extract of matico. We demonstrated that matico extract at low concentrations and in a concentration dependent manner (0.05–1 mg/mL) was a potent inhibitor of platelet aggregation in response to collagen, convulsion and ADP (IC50 values was 61 μg/mL, 72 μg/mL and 290 μg/mL, respectively). In this sense matico extract exerted the greatest antiaggregant activity induced by collagen. Similarly, matico showed a decrease in % of positive platelet for P-selectina (vehicle, 0.01, 0.05, 0.1, 0.5 and 1 mg/mL were 32 ± 2%, 29 ± 2 (p < 0.05), 19 ± 1 (p < 0.01), 15 ± 2 (p < 0.01), 10 ± 1% (p < 0.01) and 7 ± 2% (p < 0.01), respectively) and PAC-1 binding (vehicle, 0.01, 0.05, 0.1, 0.5 and 1 mg/mL were 59 ± 1, 58 ± 3 (n.s), 55 ± 2 (p < 0.05), 50 ± 2 (p < 0.01), 38 ± 1 (p < 0.01), 36 ± 2 (p < 0.01). The cellular mechanism for the antiplatelet activity of matico might be mediated by the inhibition of phospholipase C-gamma 2 and protein kinase C phosphorylation. This beneficial property of matico may be of importance in thrombosis, in which platelet activation and aggregation are important determinants of thrombus initiation and development, and may contribute to the beneficial effects of matico intake in the prevention of cardiovascular diseases.

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Section: Discussionmentioning

confidence: 99%

Buddleja globosa (matico) prevents collagen-induced platelet activation by decreasing phospholipase C-gamma 2 and protein kinase C phosphorylation signaling

Fuentes

Sepúlveda

Alarcón

et al. 2018

Journal of Traditional and Complementary Medicine

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“…Vitor et al (2009) and Okoye et al (2014) reported that α-amyrin acetate ( 1 ) and α-amyrin ( 2 ) exhibited pronounced anti-inflammatory effects and suppressed the levels of inflammatory cytokines and COX-2 levels via inhibition of NF-κB activity and of signalling pathways involving phospho-cyclic AMP response element binding protein (Vitor et al 2009; Okoye et al 2014). Moreover, 2 inhibited platelet aggregation induced by adenosine 5′-diphosphate, collagen and arachidonic acid (Aragão et al 2007). Qi et al 2008 mentioned that stigmast-4,22-diene-3,6-dione ( 9 ) exhibited anti-larval activity.…”

Section: Discussionmentioning

confidence: 99%

New ursane triterpenoids from Ficus pandurata and their binding affinity for human cannabinoid and opioid receptors

et al. 2016

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Phytochemical investigation of Ficus pandurata Hance (Moraceae) fruits has led to the isolation of two new triterpenoids, ficupanduratin A [1β-hydroxy-3β-acetoxy-11α-methoxy-urs-12-ene] (11) and ficupanduratin B [21α-hydroxy-3β-acetoxy-11α-methoxy-urs-12-ene] (17), along with 20 known compounds: α-amyrin acetate (1), α-amyrin (2), 3β-acetoxy-20-taraxasten-22-one (3), 3β-acetoxy-11α-methoxy-olean-12-ene (4), 3β-acetoxy-11α-methoxy-12-ursene (5), 11-oxo-α-amyrin acetate (6), 11-oxo-β-amyrin acetate (7), palmitic acid (8), stigmast-4,22-diene-3,6-dione (9), stigmast-4-ene-3,6-dione (10), stigmasterol (12), β-sitosterol (13), stigmast-22-ene-3,6-dione (14), stigmastane-3,6-dione (15), 3β,21β-dihydroxy-11α-methoxy-olean-12-ene (16), 3β-hydroxy-11α-methoxyurs-12-ene (18), 6-hydroxystigmast-4,22-diene-3-one (19), 6-hydroxystigmast-4-ene-3-one (20), 11α,21α-dihydroxy-3β-acetoxy-urs-12-ene (21), and β-sitosterol-3-O-β-D-glucopyranoside (22). Compound 21 is reported for the first time from a natural source. The structures of the 20 compounds were elucidated on the basis of IR, 1D (1H and 13C), 2D (1H–1H COSY, HSQC, HMBC and NOESY) NMR and MS spectroscopic data, in addition to comparison with literature data. The isolated compounds were evaluated for their anti-microbial, anti-malarial, anti-leishmanial, and cytotoxic activities. In addition, their radioligand displacement affinity on opioid and cannabinoid receptors was assessed. Compounds 4, 11, and 15 exhibited good affinity towards the CB2 receptor, with displacement values of 69.7, 62.5 and 86.5 %, respectively. Furthermore, the binding mode of the active compounds in the active site of the CB2 cannabinoid receptors was investigated through molecular modelling.

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“…Plants OSCs always possess promiscuous activities, and could cyclize 2,3-oxidosqualene to different conformations simultaneously. For example, amyrin synthase from Glycyrrhiza uralensis can produce not only α-amyrin but also β-amyrin [7]. Due to the multiple-activity of OSCs, more than 100 kinds of triterpenoid skeletons could be generated during cyclization.…”

Section: Figure 1(a)mentioning

confidence: 99%

De NovoSynthesis of Plant Natural Products in Yeast

Sun¹,

Zhao²,

Li³

2019

Yeasts in Biotechnology

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Plant natural products possess versatile biological activities including antiviral, anticancer and hepatoprotective activities, which are widely used in pharmaceutical and many other health-related fields. However, current production of such compounds relies on plant culture and extraction, which brings about severe concerns for environmental, ecological and amount of agricultural lands used. With the increasing awareness of environmental sustainability and shortage of lands, yeasts are engineered to produce natural products, for its inherent advantages such as the robustness, safety and sufficient supply of precursors. This chapter focused on the recent progress of yeast as a platform for the biosynthesis of plant natural products.

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Antiplatelet Activity ofα.- andβ.-Amyrin, Isomeric Mixture fromProtium heptaphyllum.

Cited by 21 publications

References 31 publications

Buddleja globosa (matico) prevents collagen-induced platelet activation by decreasing phospholipase C-gamma 2 and protein kinase C phosphorylation signaling

Buddleja globosa (matico) prevents collagen-induced platelet activation by decreasing phospholipase C-gamma 2 and protein kinase C phosphorylation signaling

New ursane triterpenoids from Ficus pandurata and their binding affinity for human cannabinoid and opioid receptors

De NovoSynthesis of Plant Natural Products in Yeast

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